Isolated polypeptides, polynucleotides useful for modifying water user efficiency, fertilizer use efficiency, biotic/abiotic stress tolerance, yield and biomass in plants

ABSTRACT

Polynucleotides, polypeptides, plant cells expressing same and methods of using same for increasing abiotic stress tolerance water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant. The method is effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.

RELATED APPLICATIONS

This application is continuation of U.S. patent application Ser. No. 12/810,855 filed on Jun. 28, 2010, which is a National Phase of PCT Patent Application No. PCT/IL2008/001657 having International filing date of Dec. 23, 2008, which claims the benefit of priority of U.S. Provisional Patent Applications Nos. 61/136,238 filed on Aug. 20, 2008 and 61/009,166 filed on Dec. 27, 2007. The contents of the above Applications are all incorporated herein by reference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to novel aquaporin polynucleotides and polypeptides, and more particularly, but not exclusively, to methods of using same for increasing abiotic stress tolerance, water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant.

Abiotic stress conditions such as salinity, drought, flood, suboptimal temperature and toxic chemical pollution, cause substantial damage to agricultural plants. Most plants have evolved strategies to protect themselves against these conditions. However, if the severity and duration of the stress conditions are too great, the effects on plant development, growth and yield of most crop plants are profound. Furthermore, most of the crop plants are highly susceptible to abiotic stress (ABS) and thus necessitate optimal growth conditions for commercial crop yields. Continuous exposure to stress causes major alterations in the plant metabolism which ultimately leads to cell death and consequently yield losses.

The global shortage of water supply is one of the most severe agricultural problems affecting plant growth and crop yield and efforts are made to mitigate the harmful effects of desertification and salinization of the world's arable land. Thus, Agbiotech companies attempt to create new crop varieties which are tolerant to different abiotic stresses focusing mainly in developing new varieties that can tolerate water shortage for longer periods.

Studies have shown that plant adaptations to adverse environmental conditions are complex genetic traits with polygenic nature. When water supply is limited, the plant WUE is critical for the survival and yield of crop. Since water scarcity is increasing and water quality is reducing worldwide it is important to increase water productivity and plant WUE. Many of the environmental abiotic stresses, such as drought, low temperature or high salt, decrease root hydraulic conductance, affect plant growth and decrease crop productivity.

Genetic improvement of FUE in plants can be generated either via traditional breeding or via genetic engineering. Attempts to improve FUE in transgenic plants are described in U.S. Patent Applications 20020046419 to Choo, et al.; U.S. Pat. Appl. 20030233670 to Edgerton et al.; U.S. Pat. Appl. 20060179511 to Chomet et al.; Yanagisawa et al. [Proc. Natl. Acad. Sci. U.S.A. 2004, 101(20):7833-8]; Good A G et al. [Trends Plant Sci. 2004, 9(12):597-605]; and U.S. Pat. No. 6,084,153 to Good et al.

Aquaporins (AQPs), the water channel proteins, are involved in transport of water through the membranes, maintenance of cell water balance and homeostasis under changing environmental and developmental conditions [Maurel C. Plant aquaporins: Novel functions and regulation properties. FEBS Lett. 2007, 581(12):2227-36]. These proteins are considered to be the main passage enabling transport of water and small neutral solutes such as urea and CO₂ through the membrane [Maurel C. Plant aquaporins: Novel functions and regulation properties. FEBS Lett. 2007 Jun. 12; 581(12):2227-36]. In plants, AQPs are present as four subfamilies of intrinsic proteins: plasma membrane (PIP), tonoplast (TIP), small and basic (SIP) and NOD26-like (NIP). The total number of AQP members in plants, as compared to animals, appears to be surprisingly high [Maurel C., 2007 (Supra)]. For instance, 35 AQP genes have been identified in the Arabidopsis genome [Quigley F, et al., “From genome to function: the Arabidopsis aquaporins”. Genome Biol. 2002, 3(1): RESEARCH0001.1-1.17], 36 in maize [Chaumont F, et al., 2001, “Aquaporins constitute a large and highly divergent protein family in maize. Plant Physiol”, 125(3):1206-15], and 33 in rice [Sakurai, J., et al., 2005, Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiol. 46, 1568-1577]. The high number of AQPs in plants suggests a diverse role and differential regulation under variable environmental conditions [Maurel C., 2007 (Supra)].

WO2004/104162 to the present inventors teaches polynucleotide sequences and methods of utilizing same for increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass of a plant.

WO2007/020638 to the present inventors teaches polynucleotide sequences and methods of utilizing same for increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass, vigor and/or yield of a plant.

Lian H L, et al., 2006 (Cell Res. 16: 651-60) over-expressed members of the PIP1 subgroup of AQPs in rice. Aharon R., et al. 2003 (Plant Cell, 15: 439-47) over-expressed the Arabidopsis plasma membrane aquaporin, PIP1b, in transgenic tobacco plants.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present invention there is provided a method of increasing abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising a nucleic acid sequence at least 80% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857 and 2859-3051.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct, comprising the isolated polynucleotide of the invention and a promoter for directing transcription of the nucleic acid sequence.

According to an aspect of some embodiments of the present invention there is provided an isolated polypeptide, comprising an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259.

According to an aspect of some embodiments of the present invention there is provided a plant cell comprising an exogenous polypeptide having an amino acid sequence at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259.

According to an aspect of some embodiments of the present invention there is provided a plant cell comprising an exogenous polynucleotide comprising a nucleic acid sequence at least 80% homologous to the nucleic acid sequence selected from the group consisting of SEQ ID NOs:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857 and 2859-3051.

According to an aspect of some embodiments of the present invention there is provided a method of increasing abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259, thereby increasing the abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present invention there is provided a method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.

According to an aspect of some embodiments of the present invention there is provided an isolated polynucleotide comprising the nucleic acid sequence set forth by SEQ ID NO:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857, 2859-3050 or 3051.

According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct, comprising the isolated polynucleotide of the invention and a promoter for directing transcription of the nucleic acid sequence.

According to an aspect of some embodiments of the present invention there is provided an isolated polypeptide, comprising the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259.

According to an aspect of some embodiments of the present invention there is provided a plant cell comprising an exogenous polypeptide having the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259.

According to an aspect of some embodiments of the present invention there is provided a plant cell comprising an exogenous polynucleotide comprising the nucleic acid sequence set forth by SEQ ID NO:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857, 2859-3050 or 3051.

According to some embodiments of the invention, the polynucleotide is selected from the group consisting of SEQ ID NOs:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857 and 2859-3051.

According to some embodiments of the invention, the amino acid sequence is selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259.

According to some embodiments of the invention, the polypeptide is selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259.

According to some embodiments of the invention, the abiotic stress is selected from the group consisting of salinity, water deprivation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the method further comprising growing the plant expressing the exogenous polynucleotide under the abiotic stress.

According to some embodiments of the invention, the promoter is a constitutive promoter.

According to some embodiments of the invention, the plant cell forms a part of a plant.

Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting.

BRIEF DESCRIPTION OF THE DRAWINGS

Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.

In the drawings:

FIG. 1 is a schematic illustration of the pGI binary plasmid used for expressing the isolated polynucleotide sequences of the invention. RB—T-DNA right border; LB—T-DNA left border; H—HindIII restriction enzyme; X—XbaI restriction enzyme; B—BamHI restriction enzyme; S—SalI restriction enzyme; Sm—SmaI restriction enzyme; R-I—EcoRI restriction enzyme; Sc—SacI/SstI/Ecl136II; (numbers)—Length in base-pairs; NOS pro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylation signal); GUSintron—the GUS reporter gene (coding sequence and intron). The isolated polynucleotide sequences of some embodiments of the invention were cloned into the vector while replacing the GUSintron reporter gene.

FIGS. 2A-B are images depicting root development of plants grown in transparent agar plates. The different transgenes were grown in transparent agar plates for 10-15 days and the plates were photographed every 2-5 days starting at day 1. FIG. 2A—An exemplary image of plants taken following 12 days on agar plates. FIG. 2B—An exemplary image of root analysis in which the length of the root measured is represented by a red arrow.

FIGS. 3A-F are histograms depicting the total economic fruit yield, plant biomass and harvest index for TOM-ABST36 (black bar) vs. control (white bar) plants growing in the commercial greenhouse under a 200 mM sodium chloride (NaCl) irrigation regime (FIG. 3A-C, respectively), or under two different water-stress regimes (WLI-1 and WLI-2; FIG. 3D-F, respectively). Yield performance was compared to plants growing under standard irrigation conditions (0 mM NaCl and WLI-0). Results are the average of the four independent events. *Significantly different at P≦0.05.

FIGS. 3G-J are photographs of transgenic tomato plants or control plants grown under various conditions. FIG. 3G—TOM-ABST36 plants growing under regular irrigation conditions; FIG. 3H—control plants growing under regular irrigation conditions; FIG. 3I—TOM-ABST36 plants after growing under a 200-mM NaCl-irrigation regime during the entire growing season; FIG. 3J-control plants after growing under a 200-mM NaCl-irrigation regime during the entire growing season.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to novel aquaporin polynucleotides and polypeptides, and more particularly, but not exclusively, to methods of using same for increasing abiotic stress tolerance, water use efficiency, fertilizer use efficiency, biomass, vigor and/or yield of a plant.

Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways.

While reducing the invention to practice, the present inventors have identified novel aquaporin (AQP) polynucleotides and polypeptides encoded thereby.

Thus, as shown in the Examples section which follows, the present inventors have employed a bioinformatics approach which combines digital expression analysis and cross-species comparative genomics and screened 7.2 million expressed sequence tags (ESTs) from 1,195 relevant EST's libraries of both monocot and dicot plant species. Using this approach 1,114 different AQP genes have been identified and were further classified to 11 subgroups (Table 1). Further analysis revealed that ESTs of the TIP2 subgroup are significantly over-represented in both plants' roots and in plants exposed to abiotic stress (ABS), and that polypeptides (e.g., SEQ ID NOs: 27-28, 45-48, Table 2) encoded by polynucleotides of the TIP2 subgroup (e.g., SEQ ID NOs:1, 2, 19-22, Table 2) share a common consensus sequence TLXFXFAGVGS (SEQ ID NO:2826). Based on over-representation in roots, ABS conditions and tissues with low water levels (such as seed and pollen) additional polynucleotides of the aquaporin gene family were identified (SEQ ID NOs: 3-18, 23-26, Table 2), as well as homologues or orthologues thereof (SEQ ID NOs:53-1400, 2844-3051 for polynucleotides and SEQ ID NOs:1401-2746, 3052-3259 for polypeptides; Table 3). Moreover, quantitative RT-PCR analysis demonstrated increased expression of representative AQP genes (e.g., SEQ ID NOs:5, 6 and 7) under salt stress, which was higher in plants exhibiting salt tolerance as compared to plants which are sensitive to salt stress (Table 5, Example 2 of the Examples section which follows). As is further described in Examples 3-4 of the Examples section which follows, representative AQP polynucleotides were cloned (Tables 7, 8 and 9) and transgenic plants over-expressing same were generated (Example 4). These plants were shown to exhibit increased tolerance to various abiotic stresses such as osmotic stress (Tables 10-14; Example 5) and salinity stress (Tables 30-44; Example 6), increased fertilizer use efficiency (under nitrogen limiting conditions, Tables 60-69, Example 7) and increased growth, biomass and yield under normal [Tables 15-29 (Example 5), 45-59 (Example 6)] or abiotic stress conditions conditions (Examples 5-8). Altogether, these results suggest the use of the AQP polynucleotides and polypeptides of the invention for increasing abiotic stress tolerance, water use efficiency, fertilizer use efficiency, biomass, vigor and/or yield of a plant.

It should be noted that polypeptides or polynucleotides which affect (e.g., increase) plant metabolism, growth, reproduction and/or viability under stress, can also affect the plant growth, biomass, yield and/or vigor under optimal conditions.

Thus, according to one aspect of the invention, there is provided a method of increasing abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor of a plant. The method is effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid consensus sequence TLXFXFAGVGS as set forth by SEQ ID NO:2826, wherein expression of the polypeptide promotes plants' biomass/vigor and/or yield under normal or stress conditions.

It is suggested that the polypeptide's activity is structurally associated with the integrity of the above consensus sequence (SEQ ID NO:2826). In some embodiments of this aspect of the present invention, the activity is a water channel activity which typically resides in the vacuaolar membrane (tonoplast) and/or the plasma membrane of the plant cell and enables the transport of water and/or small neutral solutes such as urea, nitrates and carbon dioxide (CO₂) through the membrane.

The phrase “abiotic stress” as used herein refers to any adverse effect on metabolism, growth, reproduction and/or viability of a plant. Accordingly, abiotic stress can be induced by suboptimal environmental growth conditions such as, for example, salinity, water deprivation, flooding, freezing, low or high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, atmospheric pollution or UV irradiation. The implications of abiotic stress are discussed in the Background section.

The phrase “abiotic stress tolerance” as used herein refers to the ability of a plant to endure an abiotic stress without suffering a substantial alteration in metabolism, growth, productivity and/or viability.

As used herein the phrase “water use efficiency (WUE)” refers to the level of organic matter produced per unit of water consumed by the plant, i.e., the dry weight of a plant in relation to the plant's water use, e.g., the biomass produced per unit transpiration.

As used herein the phrase “fertilizer use efficiency” refers to the uptake, spread, absorbent, accumulation, relocation (within the plant) and use of one or more of the minerals and organic moieties absorbed from the soil, such as nitrogen, phosphates and/or potassium.

As used herein the phrase “plant biomass” refers to the amount (measured in grams of air-dry tissue) of a tissue produced from the plant in a growing season, which could also determine or affect the plant yield or the yield per growing area.

As used herein the phrase “plant yield” refers to the amount (as determined by weight/size) or quantity (numbers) of tissue produced per plant or per growing season. Hence increased yield could affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time.

As used herein the phrase “plant vigor” refers to the amount (measured by weight) of tissue produced by the plant in a given time. Hence increase vigor could determine or affect the plant yield or the yield per growing time or growing area.

As used herein the term “increasing” refers to at least about 2%, at least about 3%, at least about 4%, at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, increase in plant abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor as compared to a native plant [i.e., a plant not modified with the biomolecules (polynucleotide or polypeptides) of the invention, e.g., a non-transformed plant of the same species which is grown under the same growth conditions).

As used herein, the phrase “exogenous polynucleotide” refers to a heterologous nucleic acid sequence which may not be naturally expressed within the plant or which overexpression in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. It should be noted that the exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence of the plant.

According to some embodiments of the invention, the exogenous polynucleotide of the invention encodes a polypeptide having an amino acid sequence at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 27-28, 45-48, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561, 2449-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2484 and 2765.

Homology (e.g., percent homology) can be determined using any homology comparison software, including for example, the BlastP or TBLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters, when starting from a polypeptide sequence; or the tBLASTX algorithm (available via the NCBI) such as by using default parameters, which compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database.

Homologous sequences include both orthologous and paralogous sequences. The term “paralogous” relates to gene-duplications within the genome of a species leading to paralogous genes. The term “orthologous” relates to homologous genes in different organisms due to ancestral relationship.

One option to identify orthologues in monocot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of-interest against any sequence database, such as the publicly available NCBI database which may be found at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov. If orthologues in rice were sought, the sequence-of-interest would be blasted against, for example, the 28,469 full-length cDNA clones from Oryza sativa Nipponbare available at NCBI. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second blasts are then compared. An orthologue is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralogue (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joining tree (Hypertext Transfer Protocol://en (dot) wikipedia (dot) org/wiki/Neighbor-joining) which helps visualizing the clustering.

According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide consisting of the amino acid sequence set forth by SEQ ID NO:27-28, 45-48, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561, 2449-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2484 or 2765.

According to some embodiments of the invention the exogenous polynucleotide comprises a nucleic acid sequence which is at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1, 2, 19, 20-22, 53-55, 57-87, 89-141, 143-147, 149-195, 197-206, 208-212, 214, 1102-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 2751-2752 and 2748-2750.

Identity (e.g., percent homology) can be determined using any homology comparison software, including for example, the BlastN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters.

According to some embodiments of the invention the exogenous polynucleotide is set forth by SEQ ID NO:1, 2, 19, 20-22, 53-55, 57-87, 89-141, 143-147, 149-195, 197-206, 208-212, 214, 1102-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 2751-2752, 2748-2749, or 2750.

Notwithstanding the above, additional AQP polynucleotides and polypeptides encoded thereby are contemplated by the present teachings.

According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide having an amino acid sequence at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% homologous to SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259.

According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide consisting of the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259.

In an exemplary embodiment the exogenous polynucleotide does not encode a polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs: 1828, 1867, 1404, 1436, 1495, 1543, 1554, 1560, 2451, 2452, 2459, 2464, 2482, 2484 and 3066.

According to some embodiments of the invention, the exogenous polynucleotide is at least at least about 60%, least at least about 65%, least at least about 70%, least at least about 75% least at least about 80%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, e.g., 100% identical to SEQ ID NO:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857, 2859-3050 or 3051.

According to some embodiments of the invention, the polynucleotide is set forth by SEQ ID NO:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857, 2859-3050 or 3051.

In an exemplary embodiments the exogenous polynucleotide is not the polynucleotide set forth by SEQ ID NO: 481, 520, 56, 88, 148, 196, 207, 213, 1104, 1105, 1112, 1117, 1135, 1137 or 2858.

As used herein the term “polynucleotide” refers to a single or double stranded nucleic acid sequence which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence and/or a composite polynucleotide sequences (e.g., a combination of the above).

As used herein the phrase “complementary polynucleotide sequence” refers to a sequence, which results from reverse transcription of messenger RNA using a reverse transcriptase or any other RNA dependent DNA polymerase. Such a sequence can be subsequently amplified in vivo or in vitro using a DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to a sequence derived (isolated) from a chromosome and thus it represents a contiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers to a sequence, which is at least partially complementary and at least partially genomic. A composite sequence can include some exonal sequences required to encode the polypeptide of the present invention, as well as some intronic sequences interposing therebetween. The intronic sequences can be of any source, including of other genes, and typically will include conserved splicing signal sequences. Such intronic sequences may further include cis acting expression regulatory elements.

According to some embodiments of the invention, the polynucleotide of the invention comprises no more than 5000 nucleic acids in length. According to some embodiments of the invention, the polynucleotide of the invention comprises no more than 4000 nucleic acids in length, e.g., no more than 3000 nucleic acids, e.g., no more than 2500 nucleic acids.

Nucleic acid sequences encoding the polypeptides of the present invention may be optimized for expression. A non-limiting example of an optimized nucleic acid sequence is provided in SEQ ID NO:2751, which encodes an optimized polypeptide comprising the amino acid sequence set forth by SEQ ID NO:27. Examples of such sequence modifications include, but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the plant of interest. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure, for example as described in Sardana et al. (1996, Plant Cell Reports 15:677-681). In this method, the standard deviation of codon usage, a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 1 SDCU=n=1 N [(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A Table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance with the preferred codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization Tables such as those provided on-line at the Codon Usage Database through the NIAS (National Institute of Agrobiological Sciences) DNA bank in Japan (Hypertext Transfer Protocol://World Wide Web (dot) kazusa (dot) or (dot) jp/codon/). The Codon Usage Database contains codon usage tables for a number of different species, with each codon usage Table having been statistically determined based on the data present in Genbank.

By using the above Tables to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide sequence encoding a protein of interest can be codon optimized for that particular plant species. This is effected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing restriction sites, to create new ones at potentially useful junctions (5′ and 3′ ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, in advance of any modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically-favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example PCT Patent Application 93/07278.

Thus, the invention encompasses nucleic acid sequences described hereinabove; fragments thereof, sequences hybridizable therewith, sequences homologous thereto, sequences encoding similar polypeptides with different codon usage, altered sequences characterized by mutations, such as deletion, insertion or substitution of one or more nucleotides, either naturally occurring or man induced, either randomly or in a targeted fashion.

As mentioned, the present inventors have uncovered previously uncharacterized polypeptides which share the amino acid consensus sequence set forth by SEQ ID NO:2826.

Thus, the invention provides an isolated polypeptide having an amino acid sequence at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to an amino acid sequence selected from the group consisting of SEQ ID NO: 27-28, 45-48, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561, 2449-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2484 and 2765.

According to some embodiments of the invention, the invention provides an isolated polypeptide having an amino acid sequence at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more say 100% homologous to an amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259.

According to some embodiments of the invention, the polypeptide is set forth by SEQ ID NO: 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259.

In an exemplary embodiment the polypeptide is not the polypeptide set forth by SEQ ID NO: 1828, 1867, 1404, 1436, 1495, 1543, 1554, 1560, 2451, 2452, 2459, 2464, 2482, 2484 or 3066.

The invention also encompasses fragments of the above described polypeptides and polypeptides having mutations, such as deletions, insertions or substitutions of one or more amino acids, either naturally occurring or man induced, either randomly or in a targeted fashion.

The term “plant” as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), and plant cells, tissues and organs. The plant may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores. Plants that are particularly useful in the methods of the invention include all plants which belong to the superfamily Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogon amplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloa pyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp., Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycine javanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtia coleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia dissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia, Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryza spp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petunia spp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photinia spp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosa spp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themeda triandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vaccinium spp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop. Alternatively algae and other non-Viridiplantae can be used for the methods of the present invention.

According to some embodiments of the invention, the plant used by the method of the invention is a crop plant such as rice, maize, wheat, barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean, sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea), flax, lupinus, rapeseed, tobacco, poplar and cotton.

Expressing the exogenous polynucleotide of the invention within the plant can be effected by transforming one or more cells of the plant with the exogenous polynucleotide, followed by generating a mature plant from the transformed cells and cultivating the mature plant under conditions suitable for expressing the exogenous polynucleotide within the mature plant.

According to some embodiments of the invention, the transformation is effected by introducing to the plant cell a nucleic acid construct which includes the exogenous polynucleotide of some embodiments of the invention and at least one promoter capable of directing transcription of the exogenous polynucleotide in the plant cell. Further details of suitable transformation approaches are provided hereinbelow.

As used herein, the term “promoter” refers to a region of DNA which lies upstream of the transcriptional initiation site of a gene to which RNA polymerase binds to initiate transcription of RNA. The promoter controls where (e.g., which portion of a plant) and/or when (e.g., at which stage or condition in the lifetime of an organism) the gene is expressed.

Any suitable promoter sequence can be used by the nucleic acid construct of the present invention. Preferably the promoter is a constitutive promoter, a tissue-specific, or an abiotic stress-inducible promoter.

Suitable constitutive promoters include, for example, CaMV 35S promoter (SEQ ID NO:2825; Odell et al., Nature 313:810-812, 1985); Arabidopsis At6669 promoter (SEQ ID NO:2823; see PCT Publication No. WO04081173A2); maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last et al., Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al., Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant J November; 2(6):837-44, 1992); ubiquitin (Christensen et al, Plant Mol. Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant Mol. Biol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, Mol. Gen. Genet. 231: 276-285, 1992); Actin 2 (An et al, Plant J. 10(1); 107-121, 1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76, 1995). Other constitutive promoters include those in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597: 5,466,785; 5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to, leaf-specific promoters [such as described, for example, by Yamamoto et al., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67, 1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol. 23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993], seed-preferred promoters [e.g., from seed specific genes (Simon, et al., Plant Mol. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant Mol. Biol. 14: 633, 1990), Brazil Nut albumin (Pearson' et al., Plant Mol. Biol. 18: 235-245, 1992), legumin (Ellis, et al. Plant Mol. Biol. 10: 203-214, 1988), Glutelin (rice) (Takaiwa, et al., Mol. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al Plant Mol Biol, 143). 323-32 1990), napA (Stalberg, et al, Planta 199: 515-519, 1996), Wheat SPA (Albanietal, Plant Cell, 9: 171-184, 1997), sunflower oleosin (Cummins, et al., Plant Mol. Biol. 19: 873-876, 1992)], endosperm specific promoters [e.g., wheat LMW and HMW, glutenin-1 (Mol Gen Genet. 216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins (EMBO3:1409-15, 1984), Barley ltrl promoter, barley B1, C, D hordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet. 250:750-60, 1996), Barley DOF (Mena et al, The Plant Journal, 116(1): 53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulin Glb-1 (Wu et al, Plant Cell Physiology 39(8) 885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant Mol. Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR gene family (Plant J 12:235-46, 1997), sorgum gamma-kafirin (PMB 32:1029-35, 1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc. Natl. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma of al, Plant Mol. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant Mol. Biol. 15, 95-109, 1990), LAT52 (Twell et al Mol. Gen. Genet. 217:240-245; 1989), apetala-3].

Suitable abiotic stress-inducible promoters include, but not limited to, salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al., Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such as maize rab 17 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266, 1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295, 1997) and maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol. 39:373-380, 1999); heat-inducible promoters such as heat tomato hsp80-promoter from tomato (U.S. Pat. No. 5,187,267).

The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication. According to some embodiments of the invention, the nucleic acid construct utilized is a shuttle vector, which can propagate both in E. coli (wherein the construct comprises an appropriate selectable marker and origin of replication) and be compatible with propagation in cells. The construct according to the present invention can be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome.

The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient trait.

There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev. Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al., Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNA into plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S, and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of transgenic dicotyledonous plants.

There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. Therefore, it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants.

Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. This process permits the mass reproduction of plants having the preferred tissue expressing the fusion protein. The new generation plants which are produced are genetically identical to, and have all of the characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants' tolerance to light is gradually increased so that it can be grown in the natural environment.

According to some embodiments of the invention, the transgenic plants are generated by transient transformation of leaf cells, meristematic cells or the whole plant.

Transient transformation can be effected by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses.

Viruses that have been shown to be useful for the transformation of plant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (bean golden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); and Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261.

According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be effected by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992), Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as, for example, the American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Tatlor, Eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of an infected plant believed to contain a high concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al., Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990) 269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat protein which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA.

In one embodiment, a plant viral polynucleotide is provided in which the native coat protein coding sequence has been deleted from a viral polynucleotide, a non-native plant viral coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral polynucleotide, and ensuring a systemic infection of the host by the recombinant plant viral polynucleotide, has been inserted. Alternatively, the coat protein gene may be inactivated by insertion of the non-native polynucleotide sequence within it, such that a protein is produced. The recombinant plant viral polynucleotide may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or polynucleotide sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) polynucleotide sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one polynucleotide sequence is included. The non-native polynucleotide sequences are transcribed or expressed in the host plant under control of the subgenomic promoter to produce the desired products.

In a second embodiment, a recombinant plant viral polynucleotide is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence.

In a third embodiment, a recombinant plant viral polynucleotide is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral polynucleotide. The inserted non-native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic promoters. Non-native polynucleotide sequences may be inserted adjacent the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product.

In a fourth embodiment, a recombinant plant viral polynucleotide is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non-native coat protein coding sequence.

The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral polynucleotide to produce a recombinant plant virus. The recombinant plant viral polynucleotide or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral polynucleotide is capable of replication in the host, systemic spread in the host, and transcription or expression of foreign gene(s) (exogenous polynucleotide) in the host to produce the desired protein.

Techniques for inoculation of viruses to plants may be found in Foster and Taylor, eds. “Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)”, Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods in Virology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A. “Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A. “Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa, eds. “Principles and Techniques in Plant Virology”, Van Nostrand-Reinhold, New York.

In addition to the above, the polynucleotide of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression.

A technique for introducing exogenous polynucleotide sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous polynucleotide is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous polynucleotide molecule into the chloroplasts. The exogenous polynucleotides selected such that it is integratable into the chloroplast's genome via homologous recombination which is readily effected by enzymes inherent to the chloroplast. To this end, the exogenous polynucleotide includes, in addition to a gene of interest, at least one polynucleotide stretch which is derived from the chloroplast's genome. In addition, the exogenous polynucleotide includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous polynucleotide. Further details relating to this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference. A polypeptide can thus be produced by the protein expression system of the chloroplast and become integrated into the chloroplast's inner membrane.

Since abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor in plants can involve multiple genes acting additively or in synergy (see, for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), the present invention also envisages expressing a plurality of exogenous polynucleotides in a single host plant to thereby achieve superior effect on abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor.

Expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can than be regenerated into a mature plant using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA including all the different exogenous polynucleotide sequences. To enable co-translation of the different polypeptides encoded by the polycistronic messenger RNA, the polynucleotide sequences can be inter-linked via an internal ribosome entry site (IRES) sequence which facilitates translation of polynucleotide sequences positioned downstream of the IRES sequence. In this case, a transcribed polycistronic RNA molecule encoding the different polypeptides described above will be translated from both the capped 5′ end and the two internal IRES sequences of the polycistronic RNA molecule to thereby produce in the cell all different polypeptides. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality of different exogenous polynucleotides, can be regenerated into a mature plant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be cross-bred and resultant progeny selected for superior abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor traits, using conventional plant breeding techniques.

Thus, the invention encompasses plants exogenously expressing (as described above) the polynucleotide(s) and/or polypeptide(s) of the invention. Once expressed within the plant cell or the entire plant, the level of the polypeptide encoded by the exogenous polynucleotide can be determined by methods well known in the art such as, activity assays, Western blots using antibodies capable of specifically binding the polypeptide, Enzyme-Linked ImmunoSorbent Assay (ELISA), radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry, immunofluorescence and the like.

Methods of determining the level in the plant of the RNA transcribed from the exogenous polynucleotide are well known in the art and include, for example, Northern blot analysis, reverse transcription polymerase chain reaction (RT-PCR) analysis (including quantitative, semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization.

As mentioned, the polypeptide according to some embodiments of the invention, functions as a water channel. Thus, the invention according to some embodiments encompasses functional equivalents of the polypeptide (e.g., polypeptides capable of the biological activity of a water channel) which can be identified by functional assays (e.g., being capable of transporting water in a plant) using e.g., a cell-swelling assay (Meng, Q. X. et al. 2008. Cell Physiol Biochem, 21. pp. 123-128).

The polynucleotides and polypeptides described hereinabove can be used in a wide range of economical plants, in a safe and cost effective manner.

The effect of the transgene (the exogenous polynucleotide encoding the polypeptide) on abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor can be determined using known methods.

Abiotic Stress Tolerance—

Transformed (i.e., expressing the transgene) and non-transformed (wild type) plants are exposed to an abiotic stress condition, such as water deprivation, suboptimal temperature (low temperature, high temperature), nutrient deficiency, nutrient excess, a salt stress condition, osmotic stress, heavy metal toxicity, anaerobiosis, atmospheric pollution and UV irradiation.

Salinity Tolerance Assay—

Transgenic plants with tolerance to high salt concentrations are expected to exhibit better germination, seedling vigor or growth in high salt. Salt stress can be effected in many ways such as, for example, by irrigating the plants with a hyperosmotic solution, by cultivating the plants hydroponically in a hyperosmotic growth solution (e.g., Hoagland solution), or by culturing the plants in a hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MS medium)]. Since different plants vary considerably in their tolerance to salinity, the salt concentration in the irrigation water, growth solution, or growth medium can be adjusted according to the specific characteristics of the specific plant cultivar or variety, so as to inflict a mild or moderate effect on the physiology and/or morphology of the plants (for guidelines as to appropriate concentration see, Bernstein and Kafkafi, Root Growth Under Salinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, and reference therein).

For example, a salinity tolerance test can be performed by irrigating plants at different developmental stages with increasing concentrations of sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl) applied from the bottom and from above to ensure even dispersal of salt. Following exposure to the stress condition the plants are frequently monitored until substantial physiological and/or morphological effects appear in wild type plants. Thus, the external phenotypic appearance, degree of wilting and overall success to reach maturity and yield progeny are compared between control and transgenic plants. Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants.

Osmotic Tolerance Test—

Osmotic stress assays (including sodium chloride and mannitol assays) are conducted to determine if an osmotic stress phenotype was sodium chloride-specific or if it was a general osmotic stress related phenotype. Plants which are tolerant to osmotic stress may have more tolerance to drought and/or freezing. For salt and osmotic stress germination experiments, the medium is supplemented for example with 50 mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol. See also Example 5 of the Examples section which follows.

Drought Tolerance Assay/Osmoticum Assay—

Tolerance to drought is performed to identify the genes conferring better plant survival after acute water deprivation. To analyze whether the transgenic plants are more tolerant to drought, an osmotic stress produced by the non-ionic osmolyte sorbitol in the medium can be performed. Control and transgenic plants are germinated and grown in plant-agar plates for 4 days, after which they are transferred to plates containing 500 mM sorbitol. The treatment causes growth retardation, then both control and transgenic plants are compared, by measuring plant weight (wet and dry), yield, and by growth rates measured as time to flowering.

Conversely, soil-based drought screens are performed with plants overexpres sing the polynucleotides detailed above. Seeds from control Arabidopsis plants, or other transgenic plants overexpressing the polypeptide of the invention are germinated and transferred to pots. Drought stress is obtained after irrigation is ceased accompanied by placing the pots on absorbent paper to enhance the soil-drying rate. Transgenic and control plants are compared to each other when the majority of the control plants develop severe wilting. Plants are re-watered after obtaining a significant fraction of the control plants displaying a severe wilting. Plants are ranked comparing to controls for each of two criteria: tolerance to the drought conditions and recovery (survival) following re-watering.

Cold Stress Tolerance—

To analyze cold stress, mature (25 day old) plants are transferred to 4° C. chambers for 1 or 2 weeks, with constitutive light. Later on plants are moved back to greenhouse. Two weeks later damages from chilling period, resulting in growth retardation and other phenotypes, are compared between both control and transgenic plants, by measuring plant weight (wet and dry), and by comparing growth rates measured as time to flowering, plant size, yield, and the like.

Heat Stress Tolerance—

Heat stress tolerance is achieved by exposing the plants to temperatures above 34° C. for a certain period. Plant tolerance is examined after transferring the plants back to 22° C. for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold or heat stress.

Germination Tests—

Germination tests compare the percentage of seeds from transgenic plants that could complete the germination process to the percentage of seeds from control plants that are treated in the same manner. Normal conditions are considered for example, incubations at 22° C. under 22-hour light 2-hour dark daily cycles. Evaluation of germination and seedling vigor is conducted between 4 and 14 days after planting. The basal media is 50% MS medium (Murashige and Skoog, 1962 Plant Physiology 15, 473-497).

Germination is checked also at unfavorable conditions such as cold (incubating at temperatures lower than 10° C. instead of 22° C.) or using seed inhibition solutions that contain high concentrations of an osmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrations of salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).

Water Use Efficiency—

can be determined as the biomass produced per unit transpiration. To analyze WUE, leaf relative water content can be measured in control and transgenic plants. Fresh weight (FW) is immediately recorded; then leaves are soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) is recorded. Total dry weight (DW) is recorded after drying the leaves at 60° C. to a constant weight. Relative water content (RWC) is calculated according to the following Formula I: (FW−DW/TW−DW)×100  Formula I

Fertilizer Use Efficiency—

To analyze whether the transgenic plants are more responsive to fertilizers, plants are grown in agar plates or pots with a limited amount of fertilizer, as described, for example, in Example 6, hereinbelow and in Yanagisawa et al (Proc Natl Acad Sci USA. 2004; 101:7833-8). The plants are analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain. The parameters checked are the overall size of the mature plant, its wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the chlorophyll content of leaves (as nitrogen plant status and the degree of leaf verdure is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots, oil content, etc. Similarly, instead of providing nitrogen at limiting amounts, phosphate or potassium can be added at increasing concentrations. Again, the same parameters measured are the same as listed above. In this way, nitrogen use efficiency (NUE), phosphate use efficiency (PUE) and potassium use efficiency (KUE) are assessed, checking the ability of the transgenic plants to thrive under nutrient restraining conditions.

Nitrogen Determination—

The procedure for N (nitrogen) concentration determination in the structural parts of the plants involves the potassium persulfate digestion method to convert organic N to NO₃ ⁻ (Purcell and King 1996 Argon. J. 88:111-113, the modified Cd⁻ mediated reduction of NO₃ ⁻ to NO₂ ⁻ (Vodovotz 1996 Biotechniques 20:390-394) and the measurement of nitrite by the Griess assay (Vodovotz 1996, supra). The absorbance values are measured at 550 nm against a standard curve of NaNO₂. The procedure is described in details in Samonte et al. 2006 Agron. J. 98:168-176.

Grain Protein Concentration—

Grain protein content (g grain protein m⁻²) is estimated as the product of the mass of grain N (g grain N m⁻²) multiplied by the N/protein conversion ratio of k-5.13 (Mosse 1990, supra). The grain protein concentration is estimated as the ratio of grain protein content per unit mass of the grain (g grain protein kg⁻¹ grain).

Oil Content—

The oil content of a plant can be determined by extraction of the oil from the seed or the vegetative portion of the plant. Briefly, lipids (oil) can be removed from the plant (e.g., seed) by grinding the plant tissue in the presence of specific solvents (e.g., hexane or petroleum ether) and extracting the oil in a continuous extractor. Indirect oil content analysis can be carried out using various known methods such as Nuclear Magnetic Resonance (NMR) Spectroscopy, which measures the resonance energy absorbed by hydrogen atoms in the liquid state of the sample [See for example, Conway T F. and Earle F R., 1963, Journal of the American Oil Chemists' Society; Springer Berlin/Heidelberg, ISSN: 0003-021X (Print) 1558-9331 (Online)]; the Near Infrared (NI) Spectroscopy, which utilizes the absorption of near infrared energy (1100-2500 nm) by the sample; and a method described in WO/2001/023884, which is based on extracting oil a solvent, evaporating the solvent in a gas stream which forms oil particles, and directing a light into the gas stream and oil particles which forms a detectable reflected light.

The plant vigor can be calculated by the increase in growth parameters such as leaf area, fiber length, rosette diameter, plant fresh weight and the like per time.

The growth rate can be measured using digital analysis of growing plants. For example, images of plants growing in greenhouse on plot basis can be captured every 3 days and the rosette area can be calculated by digital analysis. Rosette area growth is calculated using the difference of rosette area between days of sampling divided by the difference in days between samples.

Measurements of seed yield can be done by collecting the total seeds from 8-16 plants together, weighting them using analytical balance and dividing the total weight by the number of plants. Seed per growing area can be calculated in the same manner while taking into account the growing area given to a single plant. Increase seed yield per growing area could be achieved by increasing seed yield per plant, and/or by increasing number of plants capable of growing in a given area.

Evaluation of the seed yield per plant can be done by measuring the amount (weight or size) or quantity (i.e., number) of dry seeds produced and harvested from 8-16 plants and divided by the number of plants.

Evaluation of growth rate can be done by measuring plant biomass produced, rosette area, leaf size or root length per time (can be measured in cm² per day of leaf area).

Fiber length can be measured using fibrograph. The fibrograph system was used to compute length in terms of “Upper Half Mean” length. The upper half mean (UHM) is the average length of longer half of the fiber distribution. The fibrograph measures length in span lengths at a given percentage point (Hypertext Transfer Protocol://World Wide Web (dot) cottoninc (dot) com/ClassificationofCotton/?Pg=4#Length).

Thus, the present invention is of high agricultural value for promoting the yield of commercially desired crops (e.g., biomass of vegetative organ such as poplar wood, or reproductive organ such as number of seeds or seed biomass).

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having” and their conjugates mean “including but not limited to”.

The term “consisting of means “including and limited to”.

The term “consisting essentially of” means that the composition, method or structure may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.

As used herein, the singular form “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a compound” or “at least one compound” may include a plurality of compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases “ranging/ranges between” a first indicate number and a second indicate number and “ranging/ranges from” a first indicate number “to” a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniques and procedures for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts.

It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments, unless the embodiment is inoperative without those elements.

Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non limiting fashion.

Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, “Molecular Cloning: A laboratory Manual” Sambrook et al., (1989); “Current Protocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., “Current Protocols in Molecular Biology”, John Wiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide to Molecular Cloning”, John Wiley & Sons, New York (1988); Watson et al., “Recombinant DNA”, Scientific American Books, New York; Birren et al. (eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis, J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), “Basic and Clinical Immunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994); Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; “Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic Acid Hybridization” Hames, B. D., and Higgins S. J., eds. (1985); “Transcription and Translation” Hames, B. D., and Higgins S. J., Eds. (1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “Immobilized Cells and Enzymes” IRL Press, (1986); “A Practical Guide to Molecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317, Academic Press; “PCR Protocols: A Guide To Methods And Applications”, Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategies for Protein Purification and Characterization—A Laboratory Course Manual” CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.

Example 1 Identification of AQP Genes Using Digital Expression Analysis and Cross-Species Comparative Genomic

The large number of AQPs in plants and the contradictory results obtained when AQPs were overexpressed in plants demonstrate the need to selectively identify the AQP genes which can improve water use efficiency (WUE) in plants, lead to increased yield and biomass under abiotic stress as well as under favorable conditions.

Under unfavorable stress conditions, some biological activities of the plant are stopped or reduced, while others, not earlier active, initiate. Still, some of the activities, which are vital for plant survival, are maintained. One hypothesis is that key genes needed for plants to maintain vital activities under unfavorable conditions would be active under broad spectrum of biotic and abiotic stresses.

To test this hypothesis and to identify the key AQP genes having the potential to improve plant performance under different biotic and/or abiotic stress conditions (e.g., salt or drought stress) a combination of digital expression analysis (also known as Electronic Northern blot) and cross-species comparative genomics was performed. The database used was available from NCBI (Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/dbEST/) and included 7.2 million expressed sequence tags (ESTs) from 1,195 relevant EST's libraries originated from 15 different species, including both monocot and dicot species, namely: Arabidopsis, barley, Brassica rapa, cotton, grape, maize, medicago, poplar, potato, rice, sorghum, soybean, sugarcane, tomato and wheat.

Tomato plants were selected as a model plant based on the high quality tomato database from several tomato species which can be used for data-mining and the present inventors' experience in using the tomato genome as a model plant. In addition, the relatively high salt tolerance exhibited by various tomato species makes the tomato genome an excellent candidate for identifying new stress tolerance mechanisms. Moreover, tomato is not only used as a model plant for genetic studies, it is also used as an important crop with well-defined yield parameters, which can be used to distinguish between genes affecting abiotic-stress tolerance and genes preventing yield loss under abiotic-stress conditions.

Gene Analysis and Data Mining—

For gene analysis and data mining the bioinformatic filtering approach used had three phases:

1. Clustering and Assembly:

EST and mRNA sequences of each of the 15 species were extracted from GenBank versions 157, 160, 161, 162, 164, 165, 166, clustered and assembled using Compugen's LEADS clustering and assembly platform (Compugen Ltd., Tel Aviv, Israel; Yelin et. al. 2003, Nature Biotechnology 21, 379-85). Automatically extracted EST library annotations were manually accurated and classified by anatomy, developmental stage, abiotic/biotic stress treatment and cultivars. The results were loaded into Oracle database. The predicted proteins were then annotated using InterPro(2) (Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/interpro/).

2. Selection of Clusters—

All clusters that contained the Major intrinsic protein domain (IPRO00425) were selected for further analysis (n=1,114).

3. Obtaining Expression Profile of the Clusters—

By digital expression approach the expression profile of all clusters was obtained in terms of plant anatomy (i.e., in what tissues/organs the gene was expressed), developmental stage (i.e., the developmental stages at which a gene can be found) and profile of treatment (provides the physiological conditions under which a gene is expressed such as drought, cold, pathogen infection, etc).

Digital expression computations was calculated as follows: over-expression fold was computed as m/(n*M/N), where “N” is total number of ESTs of specific organism; “M is number of ESTs in a given library/tissue/category; “n” is total number of ESTs in a given contig; “m” is the number of ESTs from the library/tissue/category in the contig; P-value was computed using Exact Fisher Test statistic. The combined P-value for over-expression in both Root and Abiotic stresses conditions was computed as 1−(1−p1)×(1−p2). 1,114 different AQP genes were identified in the inter species transcriptional databases. For the data mining process, the present inventors used a combination of two approaches: selection of AQP clusters showing significant over expression (EST distribution versus normal is more than two folds; statistical significance of over-expression—p Value <0.05) either in roots compared to shoots or under various abiotic stresses (including drought, cold, salinity, heat, chemical treatments, etc.), compared to non stress control. It was found that ESTs of about 9% of the AQP genes were significantly overrepresented in roots and 3.5% of them were induced under different abiotic stresses. AQP genes which are highly overrepresented in roots were selected since plants with an efficient root system are expected to capture more water from a drying soil. In addition, AQP genes which are overrepresented in various abiotic stresses such as nutrient deficiency, heat, salinity and heavy metal stresses and biotic stresses such as application of elicitors and pathogens were selected considering that they can provide high tolerance to a wide spectrum of stresses.

The same set of 1,114 AQPs was classified according to the accepted groups known in the literature: first into the four major sub-groups: PIPs, TIPs, NIPs and SIPs, and a second classification divided these four sub-groups into eleven sub-groups according to their homology in amino acid sequences. A Fisher's exact test was then used to identify subgroups having significant EST over-presentation both in roots and upon exposure to different abiotic stresses. As shown in Table 1, hereinbelow, from the eleven subgroups, only the TIP2 subgroup showed a significant EST overrepresentation both in roots and upon exposure to abiotic stresses (P-value 1.7×10⁻⁵ and 1.6×10⁻³, respectively).

TABLE 1 AQP type distribution and over-expression in roots and abiotic stresses Roots Exposure to abiotic stresses Total No. of No. of over- % over- No. of over- % over- AQP genes in expressed expressed/ P- expressed expressed/ P- type database genes all value genes all value PIP1 243 26 10.7 0.13 10 4.1 0.34 PIP2 336 25 7.4 0.87 12 3.6 0.53 PIP3 11 0 0.0 1 0 0 1 SIP1 39 0 0.0 1 0 0 1 SIP2 16 0 0.0 1 0 0 1 TIP1 152 11 7.2 0.8 3 2 0.92 TIP2 101 22 21.8 1.70E−05 10 9.9 1.6E−0.3 TIP3 29 0 0.0 1 0 0 1 TIP4 48 5 10.4 0.41 1 2.1 0.83 TIP5 3 0 0.0 1 0 0 1 NIP 136 8 5.9 0.93 3 2.2 0.88 Total 1114 97 39 Table 1.

These results suggest that over-expression and/or protein over-accumulation of the Tip2 subgroup can improve plant water use efficiency, ABST and yield.

Genes of the Tip2 Subgroup are Highly Expressed in Roots and in Abiotic Stresses—

As shown in Table 1, hereinabove, the TIP2 subgroup (or subfamily) is highly expressed in roots and in abiotic stresses. The TIP2 subgroup is found in 38 plant species and other organisms (nucleic acid SEQ ID NOs: 1, 2, 19, 20-22; Table 2), available in public databases [Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/dbEST/]. In tomato, the TIP2 gene was highly expressed in roots (6 fold, p≦1.01 E-24) and in both biotic (2 fold, p≦4.6 E-02) and abiotic stresses (4.5 fold, p≦E-02) (data not shown).

Identification of a Short Consensus Sequence of the Tip2 Sub Family—

While comparing the consensus amino-acid sequences of Aquaporins, a short consensus sequence was identified which is unique to proteins of the Tip2 sub-family. The present inventors have suggested that this motif has an important role in managing water use efficiency (WUE), and when over-expressed in a plant can confer ABST and improved yield. The amino-acid consensus sequence identified is TLXFXFAGVGS (SEQ ID NO:2826), wherein X stands for any amino acid.

In addition, other genes of the aquaporin gene family were identified by bioinformatics tools as improving ABST and yield, based on combined digital gene expression profile in roots, tissues with low water levels (such as seed and pollen) and under abiotic stress conditions. These include SEQ ID NOs: 3-18, 23-26 (Table 2).

TABLE 2 Identified Aquaporin Genes SEQ ID NO: SEQ ID NO: (Polynucleotide) Gene name Cluster name Organism (Polypeptide) 1 MAB54 tomato|gb164|BG125449 tomato 27 2 MAB55 tomato|gb164|BG134896 tomato 28 3 MAB56 tomato|gb164|AW218990 tomato 29 4 MAB57 tomato|gb164|AA824812 tomato 30 6 MAB58 tomato|gb164|AW934056 tomato 32 7 MAB69 tomato|gb164|AI637360 tomato 33 8 MAB70 tomato|gb164|BG133531 tomato 34 9 MAB71 tomato|gb164|BG629975 tomato 35 10 MAB72 tomato|gb164|BG136017 tomato 36 11 MAB73 tomato|gb164|BG131871 tomato 37 12 MAB74 tomato|gb164|AI775489 tomato 38 13 MAB75 tomato|gb164|BG136239 tomato 39 14 MAB76 tomato|gb164|BG134058 tomato 40 15 MAB77 tomato|gb164|BG629900 tomato 41 16 MAB78 tomato|gb164|BG130774 tomato 42 17 MAB79 tomato|gb164|BG124486 tomato 43 18 MAB80 tomato|gb164|AI483521 tomato 44 19 MAB81 tomato|gb164|CO751453 tomato 45 20 MAB115 barley|gb157.2|BF626376 barley 46 22 MAB117 barley|gb157.2|BE412516 barley 48 23 MAB119 tomato|gb164|BG134199 tomato 49 24 MAB176 tomato|gb164|CO635830 tomato 50 25 MAB177 tomato|gb164|CO751496 tomato 51 26 MAB178 tomato|gb164|CO751374 tomato 52 Table 2.

Sequences which are homologous [showing at least 80% protein sequence identity on 80% of the global hit or query length, as calculated using BlastP and tBlastN algorithms of the National Center of Biotechnology Information (NCBI)] or orthologues of the AQP genes described in Table 2, and are expected to possess the same role in ABST and yield improvement in plants, are disclosed in Table 3 hereinbelow (SEQ ID NOs:6, 215-1101 and 1138-1400; Table 3). In addition, Table 3 also includes homologous and orthologues of the AQP TIP2 subfamily (SEQ ID NOs:21, 53-214, 1102-1137) and additional homologous and orthologues (SEQ ID NOs:2844-3051).

TABLE 3 Polynucleotide and polypeptide sequences of AQP homologous and orthologous Polynuc. Polypep. Hom. of % SEQ ID SEQ ID SEQ ID % Query Subject NO: Cluster name Organism NO: NO: Ident. cover. cover. Algorithm 53 apple|gb157.3|CN883304_T1 apple 1401 27 84 92.3387097 100 blastp 54 apple|gb157.3|CN489003_T1 apple 1402 27 83 100 100 blastp 55 aquilegia|gb157.3| aquilegia 1403 27 85 100 100 blastp DR915168_T1 56 arabidopsis|gb165| arabidopsis 1404 27 81 99.1935484 98.8 blastp AT3G16240_T1 57 artemisia|gb164| artemisia 1405 27 80 98.7903226 99.1902834 blastp EY035829_T1 58 artemisia|gb164| artemisia 1406 27 85 62.9032258 100 blastp EY113320_T1 59 artemisia|gb164| artemisia 1407 27 81 98.7903226 99.1902834 blastp EY070770_T1 60 avocado|gb164|CV002132_T1 avocado 1408 27 84 50 100 blastp 61 b_juncea|gb164| b_juncea 1409 27 83 100 100 blastp EVGN00333108491419_T1 62 b_juncea|gb164| b_juncea 1410 27 82 53.6290323 97.0588235 blastp EVGN00503709641655_T1 63 b_juncea|gb164| b_juncea 1411 27 84 63.7096774 100 blastp EVGN01003711220829_T1 64 b_oleracea|gb161| b_oleracea 1412 27 84 100 100 blastp AM059585_T1 65 b_oleracea|gb161| b_oleracea 1413 27 82 100 100 blastp AM385334_T1 66 b_oleracea|gb161| b_oleracea 1414 27 81 100 100 blastp AM385915_T1 67 b_rapa|gb162|BG543171_T1 b_rapa 1415 27 82 100 100 blastp 68 b_rapa|gb162|BG543223_T1 b_rapa 1416 27 83 100 100 blastp 69 b_rapa|gb162|L37478_T1 b_rapa 1417 27 81 100 100 blastp 70 banana|gb160|DN238689_T1 banana 1418 27 83 76.6129032 100 blastp 71 bean|gb164|CB540614_T1 bean 1419 27 83 98.7903226 98.7903226 blastp 72 canola|gb161|EV092237_T1 canola 1420 27 84 100 100 blastp 73 canola|gb161|CN828178_T1 canola 1421 27 81 100 100 blastp 74 canola|gb161|CX188169_T1 canola 1422 27 82 100 100 blastp 75 canola|gb161|CD840590_T1 canola 1423 27 81 100 100 blastp 76 cassava|gb164|CK650415_T1 cassava 1424 27 85 100 100 blastp 77 castorbean|gb160| castorbean 1425 27 87 100 100 blastp EE254645_T1 78 centaurea|gb161| centaurea 1426 27 84 95.9677419 84.3971631 blastp EH725826_T1 79 centaurea|gb161| centaurea 1427 27 88 89.1129032 97.7876106 blastp EL932474_T1 80 cherry|gb157.2|EE488049_T1 cherry 1428 27 80 69.3548387 100 blastp 81 cichorium|gb161| cichorium 1429 27 80 100 92.8571429 blastp DT211633_T1 82 cichorium|gb161| cichorium 1430 27 87 100 100 blastp EH672622_T1 83 citrus|gb157.2|CX663669_T1 citrus 1431 27 88 98.7903226 99.1902834 blastp 84 citrus|gb157.2|CF417983_T1 citrus 1432 27 88 98.7903226 99.1902834 blastp 85 citrus|gb157.2|CK665344_T1 citrus 1433 27 88 98.7903226 99.1902834 blastp 86 citrus|gb157.2|CK665344_T2 citrus 1434 27 87 82.2580645 100 blastp 87 clover|gb162|BB908328_T1 clover 1435 27 81 69.7580645 100 blastp 88 cotton|gb164|AF009567_T1 cotton 1436 27 87 100 100 blastp 89 cowpea|gb166|FF397761_T1 cowpea 1437 27 84 100 100 blastp 90 cowpea|gb166|FC457059_T1 cowpea 1438 27 83 98.7903226 98.7903226 blastp 91 dandelion|gb161| dandelion 1439 27 85 100 100 blastp DY818755_T1 92 ginger|gb164|DY358186_T1 ginger 1440 27 80 98.3870968 99.1836735 blastp 93 ginger|gb164|DY351866_T1 ginger 1441 27 80 98.3870968 99.1836735 blastp 94 iceplant|gb164|AF133532_T1 iceplant 1442 27 81 100 100 blastp 95 ipomoea|gb157.2| ipomoea 1443 27 87 100 100 blastp BJ576630_T1 96 lettuce|gb157.2| lettuce 1444 27 87 100 100 blastp DW074363_T1 97 lettuce|gb157.2| lettuce 1445 27 85 50.8064516 90.647482 blastp DW074363_T2 98 lettuce|gb157.2| lettuce 1446 27 87 100 100 blastp DW145132_T1 99 lettuce|gb157.2| lettuce 1447 27 87 100 100 blastp DW043760_T1 100 lettuce|gb157.2| lettuce 1448 27 87 100 100 blastp DW104999_T1 101 lotus|gb157.2|BI420407_T1 lotus 1449 27 84 100 100 blastp 102 lotus|gb157.2|BF177457_T1 lotus 1450 27 86 98.7903226 98.7951807 blastp 103 medicago|gb157.2| medicago 1451 27 83 89.1129032 94.8497854 blastp AI974300_T1 104 medicago|gb157.2| medicago 1452 27 84 100 100 blastp AA660400_T1 105 nicotiana_benthamiana| nicotiana_benthamiana 1453 27 86 98.7903226 98.7903226 blastp gb162|CN741988_T1 106 nicotiana_benthamiana| nicotiana_benthamiana 1454 27 92 100 100 blastp gb162|CN655366_T1 107 nicotiana_benthamiana| nicotiana_benthamiana 1455 27 89 98.7903226 98.7903226 blastp gb162|CN741998_T1 108 nicotiana_benthamiana| nicotiana_benthamiana 1456 27 93 100 100 blastp gb162|CN742343_T1 109 peach|gb157.2|BU045214_T1 peach 1457 27 82 100 100 blastp 110 pepper|gb157.2| pepper 1458 27 84 61.6935484 100 blastp CO776446_T1 111 pepper|gb157.2| pepper 1459 27 86 63.3064516 100 blastp CA518313_T1 112 periwinkle|gb164| periwinkle 1460 27 88 99.1935484 99.1935484 blastp EG555051_T1 113 petunia|gb157.2| petunia 1461 27 84 63.7096774 85.2517986 tblastn CV296219_T1 114 poplar|gb157.2|BI129443_T1 poplar 1462 27 86 100 100 blastp 115 poplar|gb157.2|AI166943_T2 poplar 1463 27 83 61.6935484 100 blastp 116 poplar|gb157.2|AI166943_T1 poplar 1464 27 85 100 100 blastp 117 poplar|gb157.2|BI127662_T1 poplar 1465 27 89 79.0322581 100 blastp 118 potato|gb157.2|BQ513382_T1 potato 1466 27 97 100 100 blastp 119 potato|gb157.2|BQ513382_T2 potato 1467 27 97 50.8064516 96.1832061 blastp 120 radish|gb164|EV538411_T1 radish 1468 27 82 92.3387097 100 blastp 121 radish|gb164|AB010416_T1 radish 1469 27 81 100 100 blastp 122 radish|gb164|EW725945_T1 radish 1470 27 82 100 100 blastp 123 radish|gb164|EV527946_T1 radish 1471 27 81 100 100 blastp 124 rose|gb157.2|BQ104096_T1 rose 1472 27 85 85.0806452 95.045045 blastp 125 safflower|gb162| safflower 1473 27 87 100 100 blastp EL374001_T1 126 safflower|gb162| safflower 1474 27 83 100 100 blastp EL406178_T1 127 sesame|gb157.2| sesame 1475 27 81 61.2903226 86.3636364 tblastn BU668161_T1 128 soybean|gb166|AW349399_T1 soybean 1476 27 84 98.7903226 98.7903226 blastp 129 soybean|gb166|CD416937_T1 soybean 1477 27 85 75.4032258 100 blastp 130 soybean|gb166|CA786095_T1 soybean 1478 27 84 98.7903226 98.7903226 blastp 131 soybean|gb166|AW350817_T1 soybean 1479 27 81 100 100 blastp 132 spruce|gb162|CO216479_T1 spruce 1480 27 80 98.7903226 98.4 blastp 133 strawberry|gb164| strawberry 1481 27 82 100 100 blastp DV438565_T1 134 sunflower|gb162| sunflower 1482 27 82 100 100 blastp X95952_T1 135 sunflower|gb162| sunflower 1483 27 83 100 100 blastp CD847513_T1 136 sunflower|gb162| sunflower 1484 27 84 100 100 blastp CD845750_T1 137 sunflower|gb162| sunflower 1485 27 86 100 100 blastp CD848081_T1 138 sunflower|gb162| sunflower 1486 27 83 100 100 blastp CD849577_T1 139 tobacco|gb162|CV016921_T1 tobacco 1487 27 88 100 100 blastp 140 tobacco|gb162|CV018684_T1 tobacco 1488 27 93 100 100 blastp 141 tobacco|gb162|CV019641_T1 tobacco 1489 27 91 100 100 blastp 142 tomato|gb164|AW626247_T1 tomato No 27 83 50 88.3610451 tblastn predicted protein 143 triphysaria|gb164| triphysaria 1490 27 81 100 100 blastp EX999390_T1 144 triphysaria|gb164| triphysaria 1491 27 80 100 100 blastp BM356478_T1 145 triphysaria|gb164| triphysaria 1492 27 81 81.0483871 98.0487805 blastp BM356478_T2 146 aquilegia|gb157.3| aquilegia 1493 28 86 100 100 blastp DR922172_T1 147 arabidopsis|gb165| arabidopsis 1494 28 82 98.8 98.8 blastp AT5G47450_T1 148 arabidopsis|gb165| arabidopsis 1495 28 85 99.6 99.6 blastp AT4G17340_T1 149 artemisia|gb164| artemisia 1496 28 83 69.2 100 blastp EY080612_T1 150 b_rapa|gb162|EX104899_T1 b_rapa 1497 28 84 95.2 99.1666667 blastp 151 canola|gb161|EE430505_T1 canola 1498 28 85 95.2 94.8207171 blastp 152 canola|gb161|DY017904_T1 canola 1499 28 82 98.8 98.8 blastp 153 canola|gb161|EL590702_T1 canola 1500 28 84 99.6 99.6 blastp 154 canola|gb161|CD818320_T1 canola 1501 28 85 99.6 99.6 blastp 155 cassava|gb164|DB923860_T1 cassava 1502 28 81 100 100 blastp 156 centaurea|gb161| centaurea 1503 28 84 98.8 99.1935484 blastp EL932179_T1 157 centaurea|gb161| centaurea 1504 28 82 87.6 88.9795918 blastp EH718862_T1 158 cichorium|gb161| cichorium 1505 28 86 88.8 64.7230321 tblastn EH689841_T1 159 citrus|gb157.2|CO913277_T1 citrus 1506 28 84 100 100 blastp 160 citrus|gb157.2|CO912449_T1 citrus 1507 28 82 95.6 75.2360965 tblastn 161 cotton|gb164|DV437956_T1 cotton 1508 28 88 50.8 100 blastp 162 cotton|gb164|CD486503_T1 cotton 1509 28 86 100 100 blastp 163 dandelion|gb161| dandelion 1510 28 84 100 100 blastp DY827614_T1 164 dandelion|gb161| dandelion 1511 28 86 100 100 blastp DY822865_T1 165 dandelion|gb161| dandelion 1512 28 86 100 100 blastp DY819043_T1 166 iceplant|gb164|AF133533_T1 iceplant 1513 28 82 82 99.5145631 blastp 167 lettuce|gb157.2| lettuce 1514 28 85 100 100 blastp DW057721_T1 168 lettuce|gb157.2| lettuce 1515 28 85 100 100 blastp DW045203_T1 169 lettuce|gb157.2| lettuce 1516 28 84 100 100 blastp DW046133_T1 170 lettuce|gb157.2| lettuce 1517 28 85 85.6 100 blastp DW080742_T1 171 lettuce|gb157.2| lettuce 1518 28 86 100 100 blastp DW075611_T1 172 lettuce|gb157.2| lettuce 1519 28 86 100 100 blastp DW123899_T1 173 lettuce|gb157.2| lettuce 1520 28 84 100 100 blastp DW103468_T1 174 lettuce|gb157.2| lettuce 1521 28 85 100 100 blastp DW161237_T1 175 lettuce|gb157.2| lettuce 1522 28 85 100 100 blastp DW079798_T1 176 lettuce|gb157.2| lettuce 1523 28 85 100 100 blastp DW079554_T1 177 lettuce|gb157.2| lettuce 1524 28 85 100 100 blastp DW147378_T1 178 lettuce|gb157.2| lettuce 1525 28 83 100 100 blastp DW052373_T1 179 lettuce|gb157.2| lettuce 1526 28 86 100 100 blastp DW105592_T1 180 lettuce|gb157.2| lettuce 1527 28 85 100 100 blastp DW075384_T1 181 lettuce|gb157.2| lettuce 1528 28 86 100 100 blastp DW155153_T1 182 lettuce|gb157.2| lettuce 1529 28 85 100 100 blastp CV699993_T1 183 lettuce|gb157.2| lettuce 1530 28 84 100 100 blastp DW078166_T1 184 lotus|gb157.2|AV409092_T1 lotus 1531 28 80 53.2 100 blastp 185 medicago|gb157.2| medicago 1532 28 81 98.8 99.5951417 blastp AI974377_T1 186 melon|gb165|AM725511_T1 melon 1533 28 84 100 100 blastp 187 nicotiana_benthamiana| nicotiana_benthamiana 1534 28 90 70.4 100 blastp gb162|EH370474_T1 188 onion|gb162|BE205571_T1 onion 1535 28 83 99.6 99.5967742 blastp 189 onion|gb162|AA601764_T1 onion 1536 28 86 95.2 96.3414634 blastp 190 papaya|gb165|EX255759_T1 papaya 1537 28 82 99.6 99.5983936 blastp 191 peanut|gb161|EH043676_T1 peanut 1538 28 82 99.6 99.5967742 blastp 192 pepper|gb157.2| pepper 1539 28 94 86.4 100 blastp CK901741_T1 193 periwinkle|gb164| periwinkle 1540 28 86 64.4 96.4071856 blastp FD423620_T1 194 poplar|gb157.2|BU886993_T1 poplar 1541 28 84 100 100 blastp 195 poplar|gb157.2|CA826065_T1 poplar 1542 28 85 86.8 100 blastp 196 potato|gb157.2|BG591546_T2 potato 1543 28 81 100 100 blastp 197 radish|gb164|EV531940_T1 radish 1544 28 83 94.8 94.8 blastp 198 radish|gb164|EV527785_T1 radish 1545 28 84 92.8 95.473251 blastp 199 radish|gb164|EV550763_T1 radish 1546 28 84 95.2 94.8207171 blastp 200 radish|gb164|EX902593_T1 radish 1547 28 85 95.2 99.58159 blastp 201 radish|gb164|EV535199_T1 radish 1548 28 84 96 98.7654321 blastp 202 radish|gb164|EV525705_T1 radish 1549 28 85 96 98.7654321 blastp 203 safflower|gb162| safflower 1550 28 86 86.8 100 blastp EL399548_T1 204 safflower|gb162| safflower 1551 28 87 100 100 blastp EL376421_T1 205 spurge|gb161|DV127241_T1 spurge 1552 28 85 88.4 99.103139 blastp 206 strawberry|gb164| strawberry 1553 28 82 100 100 blastp GFXDQ178022X1_T1 207 sunflower|gb162| sunflower 1554 28 86 100 100 blastp X95953_T1 208 sunflower|gb162| sunflower 1555 28 85 100 100 blastp DY911049_T1 209 sunflower|gb162| sunflower 1556 28 81 88.8 98.2300885 blastp DY921796_T1 210 sunflower|gb162| sunflower 1557 28 85 100 100 blastp DY918762_T1 211 sunflower|gb162| sunflower 1558 28 81 100 100 blastp DY906198_T1 212 sunflower|gb162| sunflower 1559 28 81 100 100 blastp DY932268_T1 213 tobacco|gb162|GFXS45406X1_T1 tobacco 1560 28 93 100 100 blastp 214 tobacco|gb162|EB445911_T1 tobacco 1561 28 89 100 100 blastp 215 apricot|gb157.2| apricot 1562 29 81 54.5454545 95.8333333 blastp CB818493_T1 216 arabidopsis|gb165| arabidopsis 1563 29 80 99.2094862 99.6031746 blastp AT4G01470_T1 217 avocado|gb164|CK760396_T1 avocado 1564 29 81 54.5454545 93.877551 blastp 218 b_rapa|gb162|EX017183_T1 b_rapa 1565 29 83 69.5652174 94.1176471 blastp 219 barley|gb157.3|BE413237_T1 barley 1566 29 81 99.2094862 99.6031746 blastp 220 cassava|gb164|CK644827_T1 cassava 1567 29 90 57.312253 99.3150685 blastp 221 cassava|gb164|BM259770_T1 cassava 1568 29 82 99.2094862 99.6031746 blastp 222 cassava|gb164|CK645124_T1 cassava 1569 29 80 99.2094862 99.6031746 blastp 223 castorbean|gb160| castorbean 1570 29 84 99.2094862 99.6031746 blastp EG666198_T1 224 castorbean|gb160| castorbean 1571 29 82 99.2094862 99.6015936 blastp AJ605571_T1 225 castorbean|gb160| castorbean 1572 29 83 99.2094862 99.6031746 blastp AJ605570_T1 226 centaurea|gb161| centaurea 1573 29 88 74.7035573 97.9274611 blastp EL931525_T1 227 cichorium|gb161| cichorium 1574 29 87 65.6126482 99.4011976 blastp EH707617_T1 228 citrus|gb157.2|CF834233_T1 citrus 1575 29 80 94.4664032 94.4444444 blastp 229 citrus|gb157.2|BQ624227_T1 citrus 1576 29 87 99.2094862 99.6031746 blastp 230 citrus|gb157.2|BQ623056_T1 citrus 1577 29 87 97.2332016 98.4 blastp 231 citrus|gb157.2|BQ624617_T1 citrus 1578 29 87 99.2094862 99.6031746 blastp 232 cotton|gb164|CD486523_T1 cotton 1579 29 81 99.2094862 99.6031746 blastp 233 cotton|gb164|AI729919_T1 cotton 1580 29 82 99.2094862 99.6031746 blastp 234 cotton|gb164|BG442315_T1 cotton 1581 29 86 99.2094862 99.6031746 blastp 235 cotton|gb164|EX167179_T1 cotton 1582 29 84 56.916996 100 blastp 236 cotton|gb164|AI726375_T1 cotton 1583 29 82 99.2094862 99.6031746 blastp 237 cowpea|gb166|FF384697_T1 cowpea 1584 29 84 99.2094862 99.6031746 blastp 238 dandelion|gb161| dandelion 1585 29 88 99.2094862 99.6031746 blastp DY825779_T1 239 fescue|gb161|CK802772_T1 fescue 1586 29 80 99.2094862 99.6031746 blastp 240 grape|gb160|BQ796848_T1 grape 1587 29 84 99.2094862 99.6015936 blastp 241 grape|gb160|CF605030_T1 grape 1588 29 82 99.2094862 99.6031746 blastp 242 ipomoea|gb157.2| ipomoea 1589 29 85 53.7549407 100 blastp EE883704_T1 243 ipomoea|gb157.2| ipomoea 1590 29 85 99.2094862 99.6031746 blastp BJ554617_T1 244 lettuce|gb157.2| lettuce 1591 29 87 99.2094862 99.6031746 blastp DW074608_T1 245 lettuce|gb157.2| lettuce 1592 29 87 99.2094862 99.6031746 blastp DY977540_T1 246 lotus|gb157.2|BW615882_T1 lotus 1593 29 80 50.1976285 100 blastp 247 maize|gb164|DQ245749_T1 maize 1594 29 81 99.2094862 99.6031746 blastp 248 medicago|gb157.2| medicago 1595 29 82 99.2094862 99.6031746 blastp BI266516_T1 249 nicotiana_benthamiana| nicotiana_benthamiana 1596 29 94 83.0039526 99.5260664 blastp gb162|CN743053_T1 250 papaya|gb165|EX256526_T1 papaya 1597 29 80 99.2094862 99.6031746 blastp 251 papaya|gb165|EX255270_T1 papaya 1598 29 86 99.2094862 99.6031746 blastp 252 peach|gb157.2|AF367456_T1 peach 1599 29 84 53.7549407 100 blastp 253 pepper|gb157.2| pepper 1600 29 81 73.1225296 98.9304813 blastp CK902019_T1 254 poplar|gb157.2|AI163470_T1 poplar 1601 29 81 99.2094862 99.6031746 blastp 255 poplar|gb157.2|AI166549_T1 poplar 1602 29 82 99.2094862 99.6031746 blastp 256 poplar|gb157.2|BU887722_T1 poplar 1603 29 81 99.2094862 99.6031746 blastp 257 poplar|gb157.2|BU875073_T1 poplar 1604 29 83 99.2094862 99.6031746 blastp 258 poplar|gb157.2|CA823737_T1 poplar 1605 29 88 53.3596838 99.2647059 blastp 259 poplar|gb157.2|AI166136_T1 poplar 1606 29 80 99.2094862 99.6031746 blastp 260 radish|gb164|EV544876_T1 radish 1607 29 80 99.2094862 99.6031746 blastp 261 rice|gb157.2|AA752956_T1 rice 1608 29 80 99.2094862 99.6031746 blastp 262 soybean|gb166|CX703984_T1 soybean 1609 29 80 99.2094862 99.6031746 blastp 263 soybean|gb166|SOYNODB_T1 soybean 1610 29 86 99.2094862 99.6031746 blastp 264 spurge|gb161|DV146067_T1 spurge 1611 29 84 87.3517787 100 blastp 265 spurge|gb161|AW990927_T1 spurge 1612 29 80 99.2094862 99.6031746 blastp 266 sunflower|gb162| sunflower 1613 29 86 99.2094862 99.6031746 blastp DY919534_T1 267 tobacco|gb162|EB443312_T1 tobacco 1614 29 92 99.2094862 99.6031746 blastp 268 tobacco|gb162|CV019217_T1 tobacco 1615 29 81 98.0237154 99.5967742 blastp 269 tobacco|gb162|EB443618_T1 tobacco 1616 29 92 99.2094862 99.6031746 blastp 270 tobacco|gb162|CV018899_T1 tobacco 1617 29 81 98.0237154 99.5967742 blastp 271 wheat|gb164|BE418306_T1 wheat 1618 29 80 99.2094862 99.6031746 blastp 272 wheat|gb164|BE404792_T1 wheat 1619 29 82 52.9644269 99.2592593 blastp 273 wheat|gb164|BE216922_T1 wheat 1620 29 81 99.2094862 99.6031746 blastp 274 artemisia|gb164| artemisia 1621 30 80 79.6 100 blastp EY083433_T1 275 banana|gb160|ES432704_T1 banana 1622 30 81 88.8 93.6708861 blastp 276 banana|gb160|DN238541_T1 banana 1623 30 80 100 100 blastp 277 barley|gb157.3|BE412510_T1 barley 1624 30 80 100 100 blastp 278 cotton|gb164|AI726168_T1 cotton 1625 30 80 98.8 99.5983936 blastp 279 cotton|gb164|AI731742_T1 cotton 1626 30 80 100 100 blastp 280 cotton|gb164|AI055329_T1 cotton 1627 30 80 100 100 blastp 281 grape|gb160|BQ794219_T1 grape 1628 30 81 100 100 blastp 282 ipomoea|gb157.2| ipomoea 1629 30 81 98 100 blastp BJ554855_T1 283 ipomoea|gb157.2| ipomoea 1630 30 84 100 100 blastp BM878761_T1 284 lettuce|gb157.2| lettuce 1631 30 80 86.8 98.1981982 blastp DW045084_T1 285 lettuce|gb157.2| lettuce 1632 30 80 88 99.103139 blastp DW114621_T1 286 lettuce|gb157.2| lettuce 1633 30 81 50.8 100 blastp DW078778_T1 287 maize|gb164|CO528320_T1 maize 1634 30 82 69.6 100 blastp 288 maize|gb164|AW257922_T1 maize 1635 30 80 52.4 100 blastp 289 maize|gb164|BI675058_T1 maize 1636 30 80 54 99.2592593 blastp 290 maize|gb164|AW352518_T1 maize 1637 30 80 51.2 100 blastp 291 maize|gb164|AF037061_T1 maize 1638 30 81 100 100 blastp 292 nicotiana_benthamiana| nicotiana_benthamiana 1639 30 90 100 100 blastp gb162|CN655062_T1 293 nicotiana_benthamiana| nicotiana_benthamiana 1640 30 90 100 100 blastp gb162|CN741621_T1 294 oil_palm|gb166| oil_palm 1641 30 80 100 100 blastp CN599861_T1 295 papaya|gb165|EX246150_T1 papaya 1642 30 82 100 100 blastp 296 pepper|gb157.2| pepper 1643 30 91 99.2 98.8 blastp BM060520_T1 297 periwinkle|gb164| periwinkle 1644 30 82 100 100 blastp EG554262_T1 298 petunia|gb157.2| petunia 1645 30 89 100 100 blastp AF452015_T1 299 potato|gb157.2|CK853059_T1 potato 1646 30 96 92 91.3043478 blastp 300 potato|gb157.2|CK852742_T1 potato 1647 30 82 60.4 100 blastp 301 potato|gb157.2|BM407759_T1 potato 1648 30 99 81.2 97.5961538 blastp 302 potato|gb157.2|CK718033_T1 potato 1649 30 98 65.2 92.0903955 blastp 303 potato|gb157.2|CK717899_T1 potato 1650 30 100 62 95.0920245 blastp 304 potato|gb157.2|CV472240_T1 potato 1651 30 97 79.6 95.215311 blastp 305 potato|gb157.2|BG098199_T1 potato 1652 30 95 93.2 99.5726496 blastp 306 rice|gb157.2|U37925_T1 rice 1653 30 81 100 100 blastp 307 rye|gb164|BE494266_T1 rye 1654 30 80 100 100 blastp 308 sorghum|gb161.xeno| sorghum 1655 30 80 100 100 blastp AF037061_T1 309 sugarcane|gb157.2| sugarcane 1656 30 80 80.4 100 blastp BQ535365_T1 310 switchgrass|gb165| switchgrass 1657 30 81 100 100 blastp DN141449_T1 311 switchgrass|gb165| switchgrass 1658 30 81 100 100 blastp DN142089_T1 312 tobacco|gb162|CN824866_T1 tobacco 1659 30 90 100 100 blastp 313 tobacco|gb162|CV017118_T1 tobacco 1660 30 90 100 100 blastp 314 wheat|gb164|TAU86762_T1 wheat 1661 30 80 100 100 blastp 315 wheat|gb164|BE499589_T1 wheat 1662 30 80 72 100 blastp 316 lettuce|gb157.2| lettuce 1663 31 80 100 100 blastp DW087170_T1 317 tobacco|gb162|EH616288_T1 tobacco 1664 32 87 50.7692308 85.1612903 blastp 318 apple|gb157.3|CO068608_T1 apple 1665 33 86 98.2638889 98.951049 blastp 319 apple|gb157.3|AB100869_T1 apple 1666 33 83 98.2638889 99.3079585 blastp 320 apple|gb157.3|AB100870_T1 apple 1667 33 83 98.2638889 99.3079585 blastp 321 apple|gb157.3|CN860225_T1 apple 1668 33 86 54.8611111 90.2857143 blastp 322 apple|gb157.3|CK900645_T1 apple 1669 33 85 98.2638889 98.951049 blastp 323 apricot|gb157.2| apricot 1670 33 89 51.0416667 98.6577181 blastp CB822297_T1 324 apricot|gb157.2| apricot 1671 33 83 98.2638889 98.9655172 blastp CB819647_T1 325 aquilegia|gb157.3| aquilegia 1672 33 86 98.2638889 98.9547038 blastp DR917005_T1 326 arabidopsis|gb165| arabidopsis 1673 33 84 73.6111111 97.260274 blastp AT4G00430_T2 327 arabidopsis|gb165| arabidopsis 1674 33 86 88.1944444 84.3853821 blastp AT2G45960_T3 328 arabidopsis|gb165| arabidopsis 1675 33 87 98.2638889 98.9547038 blastp AT4G23400_T1 329 arabidopsis|gb165| arabidopsis 1676 33 86 98.2638889 98.951049 blastp AT2G45960_T1 330 arabidopsis|gb165| arabidopsis 1677 33 87 98.2638889 98.9547038 blastp AT4G00430_T1 331 arabidopsis|gb165| arabidopsis 1678 33 88 98.2638889 98.951049 blastp AT1G01620_T1 332 arabidopsis|gb165| arabidopsis 1679 33 85 98.2638889 98.951049 blastp AT3G61430_T1 333 arabidopsis|gb165| arabidopsis 1680 33 86 88.1944444 92.7007299 blastp AT2G45960_T4 334 artemisia|gb164| artemisia 1681 33 86 98.2638889 98.9547038 blastp EY046087_T1 335 artemisia|gb164| artemisia 1682 33 87 73.6111111 99.0697674 blastp EY046310_T1 336 artemisia|gb164| artemisia 1683 33 84 97.5694444 98.2758621 blastp EY032836_T1 337 artemisia|gb164| artemisia 1684 33 84 89.2361111 99.2307692 blastp EY031810_T1 338 avocado|gb164|CK751385_T1 avocado 1685 33 86 98.2638889 98.9547038 blastp 339 avocado|gb164|CK745633_T1 avocado 1686 33 91 73.6111111 99.0654206 blastp 340 b_juncea|gb164| b_juncea 1687 33 87 98.2638889 98.951049 blastp EVGN00081008450640_T1 341 b_juncea|gb164| b_juncea 1688 33 90 60.4166667 96.1325967 blastp EVGN00515811862066_T1 342 b_juncea|gb164| b_juncea 1689 33 89 73.6111111 99.0654206 blastp EVGN00230716760965_T1 343 b_juncea|gb164| b_juncea 1690 33 84 66.3194444 96.4646465 blastp EVGN01776308261252_T1 344 b_juncea|gb164| b_juncea 1691 33 91 65.2777778 98.9473684 blastp EVGN00910030360678_T1 345 b_juncea|gb164| b_juncea 1692 33 88 51.0416667 98.6577181 blastp EVGN03812526911787_T1 346 b_juncea|gb164| b_juncea 1693 33 91 65.2777778 98.9473684 blastp EVGN00227203510305_T1 347 b_juncea|gb164| b_juncea 1694 33 87 98.2638889 98.951049 blastp EVGN00462518410866_T1 348 b_juncea|gb164| b_juncea 1695 33 89 73.2638889 99.0610329 blastp EVGN00248411120906_T1 349 b_juncea|gb164| b_juncea 1696 33 86 98.2638889 98.2638889 blastp EF471211_T1 350 b_juncea|gb164| b_juncea 1697 33 86 98.2638889 98.951049 blastp EVGN00440012650683_T1 351 b_juncea|gb164| b_juncea 1698 33 86 98.2638889 98.951049 blastp EVGN00452211183349_T1 352 b_juncea|gb164| b_juncea 1699 33 89 57.6388889 93.258427 blastp EVGN03595331210044_T1 353 b_juncea|gb164| b_juncea 1700 33 87 98.2638889 98.951049 blastp EVGN00088009631302_T1 354 b_juncea|gb164| b_juncea 1701 33 85 51.7361111 93.907563 tblastn EVGN00512912541009_T1 355 b_juncea|gb164| b_juncea 1702 33 84 69.0972222 90.3177005 tblastn EVGN00756014550623_T1 356 b_oleracea|gb161| b_oleracea 1703 33 86 98.2638889 98.951049 blastp AF299051_T1 357 b_oleracea|gb161| b_oleracea 1704 33 87 75.3472222 99.5412844 blastp AM391520_T1 358 b_oleracea|gb161| b_oleracea 1705 33 87 98.2638889 98.951049 blastp AM058918_T1 359 b_oleracea|gb161| b_oleracea 1706 33 86 98.2638889 98.951049 blastp AF299050_T1 360 b_oleracea|gb161| b_oleracea 1707 33 86 98.2638889 98.951049 blastp DY029936_T1 361 b_oleracea|gb161| b_oleracea 1708 33 87 78.4722222 100 blastp EH422530_T1 362 b_rapa|gb162|CV546930_T1 b_rapa 1709 33 84 71.5277778 99.5169082 blastp 363 b_rapa|gb162|BG544387_T1 b_rapa 1710 33 86 95.4861111 99.2779783 blastp 364 b_rapa|gb162|CA992432_T1 b_rapa 1711 33 86 98.2638889 98.951049 blastp 365 b_rapa|gb162|CV546129_T2 b_rapa 1712 33 83 54.8611111 99.3710692 blastp 366 b_rapa|gb162|EE526280_T1 b_rapa 1713 33 87 98.2638889 98.951049 blastp 367 b_rapa|gb162|L33552_T1 b_rapa 1714 33 86 98.2638889 98.951049 blastp 368 b_rapa|gb162|BG543719_T1 b_rapa 1715 33 84 77.0833333 99.5515695 blastp 369 b_rapa|gb162|AF004293_T1 b_rapa 1716 33 87 98.2638889 98.951049 blastp 370 b_rapa|gb162|BG544086_T1 b_rapa 1717 33 87 98.2638889 98.951049 blastp 371 b_rapa|gb162|CX267412_T1 b_rapa 1718 33 86 98.2638889 99.2982456 blastp 372 b_rapa|gb162|CV546129_T1 b_rapa 1719 33 86 98.2638889 98.2638889 blastp 373 b_rapa|gb162|CV545634_T1 b_rapa 1720 33 83 56.5972222 90.5555556 blastp 374 banana|gb160|DN238827_T1 banana 1721 33 84 60.7638889 99.4285714 blastp 375 banana|gb160|ES431094_T1 banana 1722 33 89 63.8888889 98.9247312 blastp 376 barley|gb157.3|BE412959_T2 barley 1723 33 83 98.2638889 98.9726027 blastp 377 barley|gb157.3|AL507831_T1 barley 1724 33 85 99.3055556 99.6551724 blastp 378 barley|gb157.3|BE412959_T1 barley 1725 33 83 98.2638889 98.9726027 blastp 379 barley|gb157.3|BE412959_T5 barley 1726 33 82 98.2638889 98.9830508 blastp 380 barley|gb157.3|BE412959_T4 barley 1727 33 82 98.2638889 98.9830508 blastp 381 barley|gb157.3|AL502020_T1 barley 1728 33 82 98.2638889 98.9726027 blastp 382 barley|gb157.3|BE412972_T1 barley 1729 33 85 98.2638889 98.9583333 blastp 383 basilicum|gb157.3| basilicum 1730 33 88 61.8055556 99.4413408 blastp DY340092_T1 384 basilicum|gb157.3| basilicum 1731 33 87 73.9583333 99.5327103 blastp DY332264_T1 385 bean|gb164|CB543592_T1 bean 1732 33 88 98.2638889 98.9547038 blastp 386 bean|gb164|PVU97023_T1 bean 1733 33 84 98.2638889 99.3079585 blastp 387 bean|gb164|CB542193_T1 bean 1734 33 84 98.6111111 99.6539792 blastp 388 beet|gb162|BVU60149_T1 beet 1735 33 84 98.2638889 98.951049 blastp 389 brachypodium|gb161.xeno| brachypodium 1736 33 83 82.9861111 95.6521739 blastp BE443278_T1 390 brachypodium|gb161.xeno| brachypodium 1737 33 85 98.2638889 98.9583333 blastp BE216990_T1 391 brachypodium|gb161.xeno| brachypodium 1738 33 82 86.8055556 72.7011494 blastp BE403307_T1 392 canola|gb161|CN731957_T1 canola 1739 33 86 98.2638889 98.951049 blastp 393 canola|gb161|CX194503_T1 canola 1740 33 86 98.2638889 98.951049 blastp 394 canola|gb161|CD814405_T1 canola 1741 33 87 98.2638889 98.951049 blastp 395 canola|gb161|EG020906_T1 canola 1742 33 86 98.2638889 98.951049 blastp 396 canola|gb161|H74720_T1 canola 1743 33 86 98.2638889 98.951049 blastp 397 canola|gb161|CN831315_T1 canola 1744 33 86 84.0277778 93.4362934 blastp 398 canola|gb161|CD817408_T1 canola 1745 33 87 98.2638889 98.951049 blastp 399 canola|gb161|EE502121_T1 canola 1746 33 89 52.7777778 98.7096774 blastp 400 canola|gb161|CX187544_T1 canola 1747 33 87 98.2638889 98.951049 blastp 401 canola|gb161|CD822064_T1 canola 1748 33 86 98.2638889 98.951049 blastp 402 canola|gb161|CD824965_T1 canola 1749 33 81 92.0138889 93.0313589 blastp 403 canola|gb161|EE485551_T1 canola 1750 33 87 98.2638889 98.951049 blastp 404 canola|gb161|CB686274_T1 canola 1751 33 86 98.2638889 98.951049 blastp 405 canola|gb161|CD814573_T1 canola 1752 33 83 76.3888889 94.0425532 blastp 406 canola|gb161|CX193398_T1 canola 1753 33 86 98.2638889 98.2638889 blastp 407 canola|gb161|CD818853_T1 canola 1754 33 86 98.2638889 98.951049 blastp 408 canola|gb161|DY005979_T1 canola 1755 33 85 78.4722222 99.5594714 blastp 409 canola|gb161|EE464964_T1 canola 1756 33 85 81.5972222 99.5762712 blastp 410 cassava|gb164|BM260264_T1 cassava 1757 33 85 97.9166667 99.3031359 blastp 411 cassava|gb164|CK901165_T1 cassava 1758 33 87 73.6111111 99.0697674 blastp 412 cassava|gb164|CK642415_T1 cassava 1759 33 87 98.2638889 98.9547038 blastp 413 cassava|gb164|DV455398_T1 cassava 1760 33 85 97.9166667 99.3031359 blastp 414 castorbean|gb160| castorbean 1761 33 89 98.2638889 98.9547038 blastp T14819_T1 415 castorbean|gb160| castorbean 1762 33 86 98.2638889 98.951049 blastp EG691229_T1 416 castorbean|gb160| castorbean 1763 33 87 97.9166667 99.3031359 blastp AJ605566_T1 417 castorbean|gb160| castorbean 1764 33 84 98.2638889 99.3055556 blastp MDL29969M000266_T1 418 castorbean|gb160| castorbean 1765 33 87 97.9166667 98.9583333 blastp AJ605574_T1 419 centaurea|gb161| centaurea 1766 33 82 97.5694444 98.6062718 blastp EH732068_T1 420 cichorium|gb161| cichorium 1767 33 87 98.2638889 98.9547038 blastp EH673032_T1 421 cichorium|gb161| cichorium 1768 33 90 51.7361111 98.6842105 blastp EH706808_T1 422 cichorium|gb161| cichorium 1769 33 86 64.9305556 95.4314721 blastp EH701938_T1 423 citrus|gb157.2|CO912471_T1 citrus 1770 33 82 69.4444444 99.5098039 blastp 424 citrus|gb157.2|BQ624312_T1 citrus 1771 33 88 98.2638889 98.9547038 blastp 425 citrus|gb157.2|CN182376_T1 citrus 1772 33 84 92.7083333 94.0559441 blastp 426 citrus|gb157.2|CB291370_T1 citrus 1773 33 89 98.2638889 98.9547038 blastp 427 citrus|gb157.2|CF833327_T1 citrus 1774 33 85 98.2638889 99.3031359 blastp 428 citrus|gb157.2|BQ624860_T1 citrus 1775 33 85 97.9166667 99.6503497 blastp 429 citrus|gb157.2|CF508404_T1 citrus 1776 33 81 97.9166667 99.6503497 blastp 430 citrus|gb157.2|CB293694_T1 citrus 1777 33 84 98.2638889 99.3031359 blastp 431 citrus|gb157.2|BQ622975_T1 citrus 1778 33 85 97.9166667 99.6503497 blastp 432 citrus|gb157.2|BE213453_T1 citrus 1779 33 86 73.6111111 99.5305164 blastp 433 citrus|gb157.2|CF828110_T1 citrus 1780 33 85 98.6111111 99.6527778 blastp 434 citrus|gb157.2|BQ623397_T1 citrus 1781 33 86 93.4027778 99.6336996 blastp 435 clover|gb162|BB903117_T1 clover 1782 33 85 98.6111111 99.6539792 blastp 436 coffea|gb157.2|BQ449035_T1 coffea 1783 33 88 98.9583333 99.3055556 blastp 437 coffea|gb157.2|DV663743_T1 coffea 1784 33 85 98.2638889 98.9473684 blastp 438 cotton|gb164|CD486529_T1 cotton 1785 33 83 98.2638889 99.3055556 blastp 439 cotton|gb164|BE052445_T1 cotton 1786 33 87 98.2638889 98.9547038 blastp 440 cotton|gb164|AI726690_T1 cotton 1787 33 86 98.2638889 98.9547038 blastp 441 cotton|gb164|DN803576_T1 cotton 1788 33 83 87.8472222 98.828125 blastp 442 cotton|gb164|BM358242_T1 cotton 1789 33 81 98.2638889 99.2882562 blastp 443 cotton|gb164|CO085369_T1 cotton 1790 33 81 52.7777778 99.3506494 blastp 444 cotton|gb164|CO098674_T1 cotton 1791 33 84 98.2638889 99.3055556 blastp 445 cotton|gb164|CO070796_T1 cotton 1792 33 84 98.2638889 99.3031359 blastp 446 cotton|gb164|AI729945_T1 cotton 1793 33 85 98.2638889 99.3079585 blastp 447 cotton|gb164|DW496760_T1 cotton 1794 33 86 80.5555556 98.3122363 blastp 448 cowpea|gb166|FF384916_T1 cowpea 1795 33 82 98.2638889 99.3031359 blastp 449 cowpea|gb166|FF555791_T1 cowpea 1796 33 82 98.6111111 99.6539792 blastp 450 cowpea|gb166|FC457489_T1 cowpea 1797 33 87 98.2638889 98.9547038 blastp 451 cowpea|gb166|AB037241_T1 cowpea 1798 33 84 98.2638889 99.3079585 blastp 452 cryptomeria|gb166| cryptomeria 1799 33 84 71.5277778 99.5215311 blastp DC429824_T1 453 cryptomeria|gb166| cryptomeria 1800 33 85 98.6111111 99.6515679 blastp AU036730_T1 454 dandelion|gb161| dandelion 1801 33 84 82.2916667 93.7007874 blastp DY814032_T1 455 dandelion|gb161| dandelion 1802 33 82 98.2638889 99.3031359 blastp DY802714_T1 456 dandelion|gb161| dandelion 1803 33 84 95.8333333 99.6415771 blastp DY822683_T1 457 dandelion|gb161| dandelion 1804 33 87 98.2638889 98.9583333 blastp DY806788_T1 458 dandelion|gb161| dandelion 1805 33 84 84.0277778 99.5918367 blastp DY808781_T1 459 dandelion|gb161| dandelion 1806 33 81 76.3888889 94.8497854 blastp DY810613_T1 460 fescue|gb161|CK803261_T1 fescue 1807 33 85 97.9166667 98.6111111 blastp 461 fescue|gb161|DT682664_T1 fescue 1808 33 84 67.3611111 99.4923858 blastp 462 fescue|gb161|DT677062_T1 fescue 1809 33 83 98.2638889 98.9655172 blastp 463 fescue|gb161|DT679061_T1 fescue 1810 33 86 98.2638889 98.9619377 blastp 464 flax|gb157.3|CV478314_T1 flax 1811 33 84 71.875 99.5260664 blastp 465 ginger|gb164|DY345344_T1 ginger 1812 33 88 98.2638889 98.951049 blastp 466 ginger|gb164|DY358322_T1 ginger 1813 33 85 98.2638889 98.9473684 blastp 467 ginger|gb164|DY360757_T1 ginger 1814 33 84 98.6111111 99.6478873 blastp 468 ginger|gb164|DY345596_T1 ginger 1815 33 86 98.2638889 98.9473684 blastp 469 grape|gb160|AF188844_T1 grape 1816 33 87 98.2638889 98.9547038 blastp 470 grape|gb160|AF188843_T1 grape 1817 33 85 98.2638889 98.951049 blastp 471 grape|gb160|AF188843_T3 grape 1818 33 85 98.2638889 98.951049 blastp 472 grape|gb160|CB971128_T1 grape 1819 33 87 98.2638889 99.3006993 blastp 473 grape|gb160|AF188843_T4 grape 1820 33 84 72.2222222 86.3070539 blastp 474 iceplant|gb164| iceplant 1821 33 85 98.2638889 98.9473684 blastp MCU26537_T1 475 iceplant|gb164|CIPMIPA_T1 iceplant 1822 33 85 98.2638889 99.2957746 blastp 476 iceplant|gb164|CIPMIPB_T1 iceplant 1823 33 87 98.2638889 98.9473684 blastp 477 ipomoea|gb157.2| ipomoea 1824 33 87 98.9583333 99.3031359 blastp BM878883_T1 478 ipomoea|gb157.2| ipomoea 1825 33 85 98.6111111 99.6491228 blastp BJ553988_T1 479 ipomoea|gb157.2| ipomoea 1826 33 84 98.6111111 99.6478873 blastp BJ553369_T1 480 ipomoea|gb157.2| ipomoea 1827 33 89 98.9583333 99.3031359 blastp BJ553198_T1 481 lettuce|gb157.2| lettuce 1828 33 86 98.2638889 97.9310345 blastp DW079915_T1 482 lettuce|gb157.2| lettuce 1829 33 87 98.2638889 98.9547038 blastp DW043941_T1 483 lettuce|gb157.2| lettuce 1830 33 87 96.875 98.245614 blastp DW047538_T1 484 lettuce|gb157.2| lettuce 1831 33 84 98.2638889 99.3031359 blastp DW104582_T1 485 lettuce|gb157.2| lettuce 1832 33 84 98.2638889 99.3031359 blastp DW044606_T1 486 lettuce|gb157.2| lettuce 1833 33 85 89.2361111 94.1605839 blastp DW148209_T1 487 lettuce|gb157.2| lettuce 1834 33 83 98.6111111 95.3333333 blastp DW148478_T1 488 lettuce|gb157.2| lettuce 1835 33 86 98.2638889 98.9547038 blastp DW108503_T1 489 lettuce|gb157.2| lettuce 1836 33 86 96.875 99.6441281 blastp DW046100_T1 490 lettuce|gb157.2| lettuce 1837 33 85 98.2638889 99.3031359 blastp DW075079_T1 491 lettuce|gb157.2| lettuce 1838 33 87 98.2638889 98.9547038 blastp DW076402_T1 492 lettuce|gb157.2| lettuce 1839 33 86 89.5833333 92.8315412 blastp DW084041_T1 493 lettuce|gb157.2| lettuce 1840 33 87 98.2638889 98.9547038 blastp DW145601_T1 494 lettuce|gb157.2| lettuce 1841 33 86 98.2638889 98.9547038 blastp CV699980_T1 495 lettuce|gb157.2| lettuce 1842 33 87 98.2638889 98.9547038 blastp DW064849_T1 496 lettuce|gb157.2| lettuce 1843 33 83 98.6111111 89.0965732 blastp DW147179_T1 497 lettuce|gb157.2| lettuce 1844 33 83 98.6111111 97.2789116 blastp DW045991_T1 498 lotus|gb157.2|AF145707_T1 lotus 1845 33 84 98.2638889 99.3079585 blastp 499 lotus|gb157.2|AI967594_T1 lotus 1846 33 86 96.875 98.245614 blastp 500 lotus|gb157.2|AF145708_T1 lotus 1847 33 87 66.6666667 100 blastp 501 maize|gb164|EC881658_T1 maize 1848 33 86 54.5138889 98.7421384 blastp 502 maize|gb164|AI372377_T1 maize 1849 33 85 98.2638889 98.9583333 blastp 503 maize|gb164|AF145706_T1 maize 1850 33 83 60.7638889 100 blastp 504 maize|gb164|AI855222_T1 maize 1851 33 84 98.2638889 98.9726027 blastp 505 maize|gb164|AI619392_T1 maize 1852 33 86 98.2638889 98.9619377 blastp 506 maize|gb164|AI861086_T1 maize 1853 33 84 98.2638889 98.9583333 blastp 507 medicago|gb157.2| medicago 1854 33 84 98.2638889 99.3079585 blastp AW684000_T1 508 medicago|gb157.2| medicago 1855 33 83 98.2638889 99.3079585 blastp AI974398_T1 509 medicago|gb157.2| medicago 1856 33 88 97.5694444 98.6062718 blastp AL366983_T1 510 medicago|gb157.2| medicago 1857 33 84 98.6111111 99.3103448 blastp AI737528_T1 511 medicago|gb157.2| medicago 1858 33 84 82.2916667 87.2262774 blastp BQ151876_T1 512 melon|gb165|DV632745_T1 melon 1859 33 84 98.2638889 99.3150685 blastp 513 melon|gb165|CF674915_T1 melon 1860 33 83 98.2638889 99.3150685 blastp 514 melon|gb165|DV632772_T1 melon 1861 33 85 98.2638889 98.951049 blastp 515 millet|gb161|CD724341_T1 millet 1862 33 83 57.2916667 92.1787709 blastp 516 nicotiana_benthamiana| nicotiana_benthamiana 1863 33 84 66.6666667 97.9487179 blastp gb162|ES885295_T1 517 oil_palm|gb166| oil_palm 1864 33 85 98.2638889 98.9547038 blastp CN600863_T1 518 oil_palm|gb166| oil_palm 1865 33 86 98.2638889 98.9547038 blastp CN600797_T1 519 onion|gb162|AF255796_T1 onion 1866 33 86 98.2638889 98.9583333 blastp 520 papaya|gb165|EX228092_T1 papaya 1867 33 84 72.2222222 93.2735426 blastp 521 papaya|gb165|AJ000031_T1 papaya 1868 33 85 98.2638889 99.3079585 blastp 522 papaya|gb165|EX257869_T1 papaya 1869 33 83 98.2638889 99.3031359 blastp 523 papaya|gb165|AM903842_T1 papaya 1870 33 90 98.2638889 98.951049 blastp 524 peach|gb157.2|BU039203_T1 peach 1871 33 84 98.2638889 98.9655172 blastp 525 peach|gb157.2|BU040913_T1 peach 1872 33 90 51.3888889 98.6666667 blastp 526 peanut|gb161|CD038184_T1 peanut 1873 33 83 98.6111111 99.6539792 blastp 527 peanut|gb161|ES490696_T1 peanut 1874 33 82 73.9583333 99.5348837 blastp 528 peanut|gb161|CD038104_T1 peanut 1875 33 83 98.2638889 99.3079585 blastp 529 pepper|gb157.2| pepper 1876 33 97 96.875 100 blastp CA523071_T1 530 pepper|gb157.2| pepper 1877 33 95 99.3055556 100 blastp BM063708_T1 531 periwinkle|gb164| periwinkle 1878 33 88 98.9583333 99.3031359 blastp EG554502_T1 532 periwinkle|gb164| periwinkle 1879 33 87 98.9583333 99.3031359 blastp EG554518_T1 533 periwinkle|gb164| periwinkle 1880 33 83 84.0277778 96.8 blastp EG556773_T1 534 petunia|gb157.2| petunia 1881 33 93 99.3055556 100 blastp AF452010_T1 535 petunia|gb157.2| petunia 1882 33 92 61.8055556 100 blastp CV292775_T1 536 petunia|gb157.2| petunia 1883 33 86 98.2638889 99.3006993 blastp AF452011_T1 537 pine|gb157.2|AL751335_T1 pine 1884 33 83 98.2638889 98.9583333 blastp 538 pine|gb157.2|AA556193_T1 pine 1885 33 85 98.2638889 98.9583333 blastp 539 pine|gb157.2|AL750485_T1 pine 1886 33 85 98.2638889 98.9583333 blastp 540 pineapple|gb157.2| pineapple 1887 33 83 98.2638889 97.9452055 blastp DT335964_T1 541 pineapple|gb157.2| pineapple 1888 33 86 98.2638889 98.9583333 blastp DT338557_T1 542 poplar|gb157.2|BU817536_T1 poplar 1889 33 83 98.2638889 99.3031359 blastp 543 poplar|gb157.2|AI162483_T1 poplar 1890 33 88 98.2638889 98.9583333 blastp 544 poplar|gb157.2|BI122420_T1 poplar 1891 33 87 97.9166667 99.3031359 blastp 545 poplar|gb157.2|AI165418_T1 poplar 1892 33 85 97.9166667 99.3031359 blastp 546 poplar|gb157.2|BU817536_T3 poplar 1893 33 82 88.1944444 92.0863309 blastp 547 poplar|gb157.2|BU881784_T1 poplar 1894 33 83 98.2638889 99.3031359 blastp 548 potato|gb157.2|BE923816_T1 potato 1895 33 85 98.2638889 99.3006993 blastp 549 potato|gb157.2|CK260061_T1 potato 1896 33 91 62.5 98.9010989 blastp 550 potato|gb157.2|BE924585_T1 potato 1897 33 86 98.2638889 98.9473684 blastp 551 potato|gb157.2|BF153976_T1 potato 1898 33 86 61.1111111 100 blastp 552 potato|gb157.2|AJ487323_T1 potato 1899 33 97 100 100 blastp 553 potato|gb157.2|BF154021_T1 potato 1900 33 92 99.3055556 100 blastp 554 potato|gb157.2|BG599633_T1 potato 1901 33 95 98.9583333 99.3031359 blastp 555 potato|gb157.2|BE922307_T1 potato 1902 33 85 98.2638889 99.3006993 blastp 556 radish|gb164|EV536875_T1 radish 1903 33 86 98.2638889 98.951049 blastp 557 radish|gb164|EW726189_T1 radish 1904 33 83 60.0694444 96.1111111 blastp 558 radish|gb164|EX756217_T1 radish 1905 33 86 98.2638889 98.951049 blastp 559 radish|gb164|AB030696_T1 radish 1906 33 86 98.2638889 98.951049 blastp 560 radish|gb164|AB030695_T1 radish 1907 33 87 98.2638889 98.951049 blastp 561 radish|gb164|AB012044_T1 radish 1908 33 85 98.2638889 98.951049 blastp 562 radish|gb164|EV567230_T1 radish 1909 33 87 98.2638889 98.9547038 blastp 563 radish|gb164|EY936735_T1 radish 1910 33 86 98.2638889 98.951049 blastp 564 rice|gb157.2|U37951_T1 rice 1911 33 86 98.2638889 98.9619377 blastp 565 rice|gb157.2|U40140_T1 rice 1912 33 86 98.2638889 98.9583333 blastp 566 rice|gb157.2|BE039992_T1 rice 1913 33 82 98.2638889 98.9583333 blastp 567 rice|gb157.2|U37951_T2 rice 1914 33 86 73.6111111 99.0654206 blastp 568 rose|gb157.2|BQ104887_T1 rose 1915 33 83 97.5694444 98.6206897 blastp 569 rose|gb157.2|BQ103877_T1 rose 1916 33 85 98.2638889 98.9547038 blastp 570 rose|gb157.2|EC586734_T1 rose 1917 33 80 62.1527778 99.4444444 blastp 571 rye|gb164|BE586240_T1 rye 1918 33 83 98.2638889 98.9726027 blastp 572 safflower|gb162| safflower 1919 33 86 89.5833333 94.5454545 blastp EL407054_T1 573 safflower|gb162| safflower 1920 33 85 98.2638889 92.5081433 blastp EL400504_T1 574 safflower|gb162| safflower 1921 33 83 84.375 99.5934959 blastp EL400004_T1 575 sesame|gb157.2| sesame 1922 33 91 57.2916667 100 blastp BU668587_T1 576 sesame|gb157.2| sesame 1923 33 87 55.2083333 99.375 blastp BU669929_T1 577 sorghum|gb161.xeno| sorghum 1924 33 85 98.2638889 98.9583333 blastp AI372377_T1 578 sorghum|gb161.xeno| sorghum 1925 33 82 98.2638889 98.9655172 blastp AI861086_T1 579 sorghum|gb161.xeno| sorghum 1926 33 86 98.2638889 98.9619377 blastp SBU87981_T1 580 soybean|gb166|CD401115_T1 soybean 1927 33 82 98.2638889 99.3031359 blastp 581 soybean|gb166|AW348556_T1 soybean 1928 33 84 98.6111111 99.6539792 blastp 582 soybean|gb166|BE352670_T1 soybean 1929 33 86 98.2638889 98.9547038 blastp 583 soybean|gb166|BI967765_T1 soybean 1930 33 86 98.2638889 98.9619377 blastp 584 soybean|gb166|BE661219_T1 soybean 1931 33 82 98.2638889 99.3079585 blastp 585 soybean|gb166|BE352747_T5 soybean 1932 33 81 88.1944444 98.4732824 blastp 586 soybean|gb166|CD416359_T1 soybean 1933 33 85 97.5694444 98.6013986 blastp 587 soybean|gb166|AW350352_T1 soybean 1934 33 84 97.5694444 98.6013986 blastp 588 soybean|gb166|BE352747_T1 soybean 1935 33 82 98.2638889 99.3079585 blastp 589 soybean|gb166|BE820629_T1 soybean 1936 33 85 98.2638889 99.2957746 blastp 590 spikemoss|gb165| spikemoss 1937 33 82 51.0416667 99.3243243 blastp DN838148_T4 591 spruce|gb162|CO224550_T1 spruce 1938 33 80 96.875 98.9473684 blastp 592 spruce|gb162|CO216100_T1 spruce 1939 33 86 98.2638889 98.9726027 blastp 593 spruce|gb162|CO216028_T1 spruce 1940 33 85 98.2638889 98.9583333 blastp 594 spurge|gb161|BG354070_T1 spurge 1941 33 87 97.9166667 98.2638889 blastp 595 strawberry|gb164| strawberry 1942 33 82 98.2638889 99.3103448 blastp CO378647_T1 596 strawberry|gb164| strawberry 1943 33 84 98.2638889 98.9547038 blastp CX661400_T1 597 strawberry|gb164| strawberry 1944 33 83 98.2638889 98.951049 blastp DV438296_T1 598 sugarcane|gb157.2| sugarcane 1945 33 80 60.0694444 88.8888889 blastp CA264801_T1 599 sugarcane|gb157.2| sugarcane 1946 33 85 98.2638889 98.9583333 blastp CA086058_T1 600 sugarcane|gb157.2| sugarcane 1947 33 84 97.2222222 98.2638889 blastp CA071197_T1 601 sugarcane|gb157.2| sugarcane 1948 33 84 91.6666667 99.2537313 blastp BQ530399_T1 602 sugarcane|gb157.2| sugarcane 1949 33 82 74.3055556 99.5391705 blastp CA085969_T1 603 sugarcane|gb157.2| sugarcane 1950 33 84 71.1805556 93.6936937 blastp CA074778_T1 604 sugarcane|gb157.2| sugarcane 1951 33 81 62.1527778 99.4505495 blastp CA130651_T1 605 sugarcane|gb157.2| sugarcane 1952 33 88 53.4722222 98.7179487 blastp AA525652_T1 606 sugarcane|gb157.2| sugarcane 1953 33 86 98.2638889 98.9619377 blastp BQ536359_T1 607 sunflower|gb162| sunflower 1954 33 86 98.2638889 98.9547038 blastp DY909123_T1 608 sunflower|gb162| sunflower 1955 33 85 98.2638889 98.9547038 blastp CD846367_T1 609 sunflower|gb162| sunflower 1956 33 86 98.2638889 98.9547038 blastp CD846084_T1 610 sunflower|gb162| sunflower 1957 33 84 72.2222222 100 blastp DY915760_T1 611 sunflower|gb162| sunflower 1958 33 83 73.6111111 99.5327103 blastp CF080940_T1 612 sunflower|gb162| sunflower 1959 33 83 96.875 97.5694444 blastp CF087907_T1 613 sunflower|gb162| sunflower 1960 33 84 98.2638889 98.6111111 blastp CX946986_T1 614 sunflower|gb162| sunflower 1961 33 87 73.6111111 99.0697674 blastp DY918780_T1 615 switchgrass|gb165| switchgrass 1962 33 86 98.2638889 98.9583333 blastp FE619753_T1 616 switchgrass|gb165| switchgrass 1963 33 85 98.2638889 98.9583333 blastp DN142591_T1 617 switchgrass|gb165| switchgrass 1964 33 85 98.2638889 98.9619377 blastp DN141716_T1 618 switchgrass|gb165| switchgrass 1965 33 86 98.2638889 98.9583333 blastp DN141343_T1 619 switchgrass|gb165| switchgrass 1966 33 85 98.2638889 98.9619377 blastp DN142037_T1 620 thellungiella|gb157.2| thellungiella 1967 33 85 98.2638889 98.951049 blastp DN774595_T1 621 thellungiella|gb157.2| thellungiella 1968 33 86 98.2638889 98.951049 blastp BM986095_T1 622 thellungiella|gb157.2| thellungiella 1969 33 85 72.5694444 99.5238095 blastp BI698563_T1 623 tobacco|gb162|EB426225_T1 tobacco 1970 33 87 98.2638889 98.2578397 blastp 624 tobacco|gb162|CK720591_T1 tobacco 1971 33 95 99.3055556 99.6515679 blastp 625 tobacco|gb162|CK720595_T1 tobacco 1972 33 96 73.6111111 99.0654206 blastp 626 tobacco|gb162|EB427872_T1 tobacco 1973 33 88 98.2638889 99.2982456 blastp 627 tobacco|gb162|CK720593_T1 tobacco 1974 33 87 97.9166667 98.9473684 blastp 628 tobacco|gb162|AF024511_T1 tobacco 1975 33 95 99.3055556 99.6515679 blastp 629 tobacco|gb162|CK720596_T1 tobacco 1976 33 96 98.9583333 99.3031359 blastp 630 tobacco|gb162|NTU62280_T1 tobacco 1977 33 96 98.9583333 99.3031359 blastp 631 tomato|gb164|AW622243_T1 tomato 1978 33 94 98.9583333 99.3031359 blastp 632 tomato|gb164|BG123213_T1 tomato 1979 33 94 99.3055556 100 blastp 633 tomato|gb164|AI637363_T1 tomato 1980 33 87 98.2638889 98.9473684 blastp 634 tomato|gb164|BG123955_T1 tomato 1981 33 85 98.2638889 99.3006993 blastp 635 tomato|gb164|BP876517_T1 tomato 1982 33 80 55.9027778 86.8705036 tblastn 636 triphysaria|gb164| triphysaria 1983 33 86 73.6111111 99.5305164 blastp BM357654_T1 637 triphysaria|gb164| triphysaria 1984 33 90 65.625 99.4736842 blastp EY141207_T1 638 triphysaria|gb164| triphysaria 1985 33 87 69.0972222 100 blastp DR174621_T1 639 triphysaria|gb164| triphysaria 1986 33 86 88.5416667 99.6108949 blastp BM356761_T1 640 triphysaria|gb164| triphysaria 1987 33 87 98.2638889 99.6466431 blastp DR169763_T1 641 triphysaria|gb164| triphysaria 1988 33 86 96.5277778 99.6428571 blastp BM356902_T1 642 triphysaria|gb164| triphysaria 1989 33 86 92.7083333 97.4545455 blastp DR174271_T1 643 triphysaria|gb164| triphysaria 1990 33 88 100 99.6539792 blastp DR171777_T1 644 wheat|gb164|BE406715_T1 wheat 1991 33 83 98.2638889 98.9726027 blastp 645 wheat|gb164|BQ838456_T1 wheat 1992 33 83 98.2638889 98.9726027 blastp 646 wheat|gb164|BE426386_T1 wheat 1993 33 82 98.2638889 98.9726027 blastp 647 wheat|gb164|BE403388_T1 wheat 1994 33 88 51.0416667 98.6577181 blastp 648 wheat|gb164|BE403307_T1 wheat 1995 33 83 98.2638889 98.9726027 blastp 649 wheat|gb164|BE498268_T1 wheat 1996 33 81 60.4166667 96.7213115 blastp 650 wheat|gb164|AL828763_T1 wheat 1997 33 84 84.7222222 96.4705882 blastp 651 wheat|gb164|AF139816_T1 wheat 1998 33 83 98.2638889 98.9726027 blastp 652 wheat|gb164|BE216990_T1 wheat 1999 33 86 98.2638889 98.9583333 blastp 653 wheat|gb164|BE403886_T1 wheat 2000 33 85 99.3055556 99.6551724 blastp 654 wheat|gb164|BE430165_T1 wheat 2001 33 85 99.3055556 99.6551724 blastp 655 wheat|gb164|CA484202_T1 wheat 2002 33 87 56.9444444 97.6190476 blastp 656 wheat|gb164|BE404199_T1 wheat 2003 33 86 98.2638889 98.9583333 blastp 657 wheat|gb164|BE406086_T1 wheat 2004 33 83 98.2638889 98.9726027 blastp 658 wheat|gb164|BF293776_T1 wheat 2005 33 83 98.2638889 98.9655172 blastp 659 wheat|gb164|CK193386_T1 wheat 2006 33 81 97.2222222 96.6216216 blastp 660 castorbean|gb160| castorbean 2007 34 80 99.1902834 98.7854251 blastp AJ605572_T1 661 citrus|gb157.2|CK740163_T1 citrus 2008 34 80 99.1902834 98.7854251 blastp 662 coffea|gb157.2|DV664793_T1 coffea 2009 34 80 100 100 blastp 663 lettuce|gb157.2| lettuce 2010 34 80 99.1902834 99.5934959 blastp DW074942_T1 664 pepper|gb157.2| pepper 2011 34 95 76.1133603 100 blastp BM063938_T1 665 periwinkle|gb164| periwinkle 2012 34 81 99.1902834 98.7903226 blastp EG558295_T1 666 potato|gb157.2|BM112462_T1 potato 2013 34 98 94.7368421 99.5744681 blastp 667 tobacco|gb162|AJ237751_T1 tobacco 2014 34 95 100 100 blastp 668 tobacco|gb162|EB425012_T1 tobacco 2015 34 93 100 100 blastp 669 nicotiana_benthamiana| nicotiana_benthamiana 2016 35 86 74.617737 97.5903614 blastp gb162|CK284579_T1 670 potato|gb157.2|BG594926_T1 potato 2017 35 96 100 96.4497041 blastp 671 tomato|gb164|DB679435_T1 tomato 2018 35 90 76.146789 100 blastp 672 tobacco|gb162|CK720588_T1 tobacco 2019 36 81 53.5580524 100 blastp 673 apple|gb157.3|CN494715_T1 apple 2020 37 84 85.7142857 94.7368421 blastp 674 apple|gb157.3|CO066689_T1 apple 2021 37 81 94.2857143 76.1538462 blastp 675 castorbean|gb160| castorbean 2022 37 90 95.2380952 36.900369 blastp MDL30026M001488_T1 676 citrus|gb157.2|CX300349_T1 citrus 2023 37 83 99.047619 72.7272727 blastp 677 coffea|gb157.2|DV663640_T1 coffea 2024 37 80 93.3333333 34.5070423 blastp 678 cowpea|gb166|FF395821_T1 cowpea 2025 37 82 93.3333333 95.1456311 blastp 679 cowpea|gb166|FF395821_T2 cowpea 2026 37 82 94.2857143 13.2450331 tblastn 680 ipomoea|gb157.2| ipomoea 2027 37 87 100 59.3220339 blastp CJ769054_T1 681 lettuce|gb157.2| lettuce 2028 37 83 98.0952381 36.5248227 blastp CV699989_T1 682 medicago|gb157.2| medicago 2029 37 80 95.2380952 37.1747212 blastp AW208262_T1 683 melon|gb165|AM727408_T1 melon 2030 37 82 97.1428571 36.9565217 blastp 684 poplar|gb157.2|CN517706_T1 poplar 2031 37 84 96.1904762 36.5942029 blastp 685 poplar|gb157.2|BU813630_T1 poplar 2032 37 84 99.047619 37.6811594 blastp 686 potato|gb157.2|DN587628_T1 potato 2033 37 90 96.1904762 93.5185185 blastp 687 rice|gb157.2|BI805522_T1 rice 2034 37 80 97.1428571 35.915493 blastp 688 soybean|gb166|FK397604_T1 soybean 2035 37 82 54.2857143 98.2758621 blastp 689 sunflower|gb162| sunflower 2036 37 82 98.0952381 39.0151515 blastp DY951259_T1 690 sunflower|gb162| sunflower 2037 37 83 98.0952381 37.3188406 blastp DY942645_T1 691 triphysaria|gb164| triphysaria 2038 37 85 99.047619 37.6811594 blastp EY130232_T1 692 arabidopsis|gb165| arabidopsis 2039 38 80 97.6271186 96.0526316 blastp AT4G10380_T1 693 artemisia|gb164| artemisia 2040 38 87 55.5932203 100 blastp EY089420_T1 694 artemisia|gb164| artemisia 2041 38 89 51.8644068 100 blastp EY113317_T1 695 b_oleracea|gb161| b_oleracea 2042 38 81 93.8983051 95.862069 blastp AM391026_T1 696 b_rapa|gb162|CV545128_T1 b_rapa 2043 38 81 97.6271186 96.013289 blastp 697 canola|gb161|ES903871_T1 canola 2044 38 85 52.2033898 100 blastp 698 cassava|gb164|CK641734_T1 cassava 2045 38 89 93.8983051 98.9247312 blastp 699 castorbean|gb160| castorbean 2046 38 85 100 100 blastp EG668085_T1 700 castorbean|gb160| castorbean 2047 38 90 62.0338983 100 blastp EG668085_T2 701 centaurea|gb161| centaurea 2048 38 83 69.1525424 98.0487805 blastp EH739099_T1 702 centaurea|gb161| centaurea 2049 38 89 80.6779661 95.9677419 blastp EH710762_T1 703 citrus|gb157.2|CX299695_T1 citrus 2050 38 98 62.0338983 100 blastp 704 citrus|gb157.2|CO912981_T1 citrus 2051 38 80 100 100 blastp 705 citrus|gb157.2|CO912981_T2 citrus 2052 38 81 78.3050847 95.5465587 blastp 706 cotton|gb164|CO071578_T1 cotton 2053 38 90 77.9661017 95.4356846 blastp 707 cotton|gb164|BE052767_T1 cotton 2054 38 88 86.440678 100 blastp 708 grape|gb160|CB350030_T1 grape 2055 38 86 100 100 blastp 709 lettuce|gb157.2| lettuce 2056 38 86 97.6271186 96.6555184 blastp DW123895_T1 710 melon|gb165|AM726471_T1 melon 2057 38 80 78.6440678 92.8 blastp 711 nicotiana_benthamiana| nicotiana_benthamiana 2058 38 94 100 100 blastp gb162|CK281387_T1 712 onion|gb162|CF436356_T1 onion 2059 38 83 86.1016949 98.0988593 blastp 713 poplar|gb157.2|BU895174_T1 poplar 2060 38 84 97.6271186 96.3333333 blastp 714 poplar|gb157.2|BI126692_T1 poplar 2061 38 85 100 100 blastp 715 radish|gb164|EX772276_T1 radish 2062 38 87 62.0338983 100 blastp 716 safflower|gb162| safflower 2063 38 87 55.5932203 94.2528736 blastp EL376221_T1 717 soybean|gb166|AW351195_T1 soybean 2064 38 83 57.2881356 100 blastp 718 spurge|gb161|DV120704_T1 spurge 2065 38 81 66.1016949 100 blastp 719 strawberry|gb164| strawberry 2066 38 87 73.559322 100 blastp EX663538_T1 720 sunflower|gb162| sunflower 2067 38 83 69.8305085 95.0636943 tblastn BQ915292_T1 721 tobacco|gb162|EB426773_T1 tobacco 2068 38 94 100 100 blastp 722 triphysaria|gb164| triphysaria 2069 38 90 67.7966102 100 blastp EY008469_T1 723 pepper|gb157.2| pepper 2070 39 91 60 100 blastp BM066463_T1 724 tobacco|gb162|EB445778_T1 tobacco 2071 39 88 85.8333333 100 blastp 725 pepper|gb157.2| pepper 2072 40 82 64.4628099 100 blastp CA515996_T1 726 potato|gb157.2|BE341068_T1 potato 2073 40 92 100 100 blastp 727 potato|gb157.2|BG887984_T1 potato 2074 41 100 79.4238683 91.4691943 blastp 728 apple|gb157.3|CN898142_T1 apple 2075 42 86 70.9677419 98.0582524 blastp 729 apple|gb157.3|CN492544_T1 apple 2076 42 82 99.6415771 98.9399293 blastp 730 apple|gb157.3|CN495819_T1 apple 2077 42 84 99.6415771 98.9547038 blastp 731 apple|gb157.3|CN869175_T1 apple 2078 42 86 100 100 blastp 732 apple|gb157.3|CN488973_T1 apple 2079 42 87 100 100 blastp 733 apricot|gb157.2| apricot 2080 42 88 69.8924731 98.4848485 blastp CB820380_T1 734 aquilegia|gb157.3| aquilegia 2081 42 85 94.9820789 100 blastp DR921860_T1 735 arabidopsis|gb165| arabidopsis 2082 42 80 100 99.2982456 blastp AT2G37170_T1 736 arabidopsis|gb165| arabidopsis 2083 42 81 95.6989247 94.7552448 blastp AT3G54820_T1 737 arabidopsis|gb165| arabidopsis 2084 42 81 100 99.3031359 blastp AT3G53420_T1 738 arabidopsis|gb165| arabidopsis 2085 42 80 99.2831541 97.2508591 blastp AT5G60660_T1 739 artemisia|gb164| artemisia 2086 42 89 79.5698925 100 blastp EY056827_T1 740 artemisia|gb164| artemisia 2087 42 83 100 100 blastp EY033689_T1 741 artemisia|gb164| artemisia 2088 42 81 100 100 blastp EY032199_T1 742 artemisia|gb164| artemisia 2089 42 88 100 100 blastp EY042731_T1 743 artemisia|gb164| artemisia 2090 42 82 99.6415771 98.9473684 blastp EX980079_T1 744 avocado|gb164|CK754546_T1 avocado 2091 42 86 51.6129032 97.2972973 blastp 745 b_juncea|gb164| b_juncea 2092 42 81 100 99.2982456 blastp EVGN00454408761136_T1 746 b_juncea|gb164| b_juncea 2093 42 81 98.9247312 97.9020979 blastp EVGN00454408761136_T2 747 b_juncea|gb164| b_juncea 2094 42 80 100 88.7850467 blastp EVGN00748222952488_T2 748 b_juncea|gb164| b_juncea 2095 42 84 82.078853 97.8991597 blastp EVGN00204411253360_T1 749 b_juncea|gb164| b_juncea 2096 42 80 100 99.2982456 blastp EVGN00054208600715_T1 750 b_juncea|gb164| b_juncea 2097 42 83 64.516129 96.2566845 blastp EVGN00049614332152_T1 751 b_juncea|gb164| b_juncea 2098 42 85 58.4229391 100 blastp EVGN01023711071914_T1 752 b_juncea|gb164| b_juncea 2099 42 81 100 99.3031359 blastp EVGN00247216171316_T1 753 b_juncea|gb164| b_juncea 2100 42 88 64.1577061 100 blastp EVGN00778009020884_T1 754 b_juncea|gb164| b_juncea 2101 42 85 53.4050179 98.6754967 blastp EVGN02648808940517_T1 755 b_juncea|gb164| b_juncea 2102 42 82 82.7956989 100 blastp EVGN00316414413452_T1 756 b_juncea|gb164| b_juncea 2103 42 81 100 99.3031359 blastp DT317706_T1 757 b_juncea|gb164| b_juncea 2104 42 81 100 99.3031359 blastp EVGN00748222952488_T1 758 b_oleracea|gb161| b_oleracea 2105 42 80 100 100 blastp AM386520_T1 759 b_oleracea|gb161| b_oleracea 2106 42 83 57.3476703 91.954023 blastp AM058395_T1 760 b_oleracea|gb161| b_oleracea 2107 42 81 100 99.2982456 blastp AM385504_T1 761 b_rapa|gb162|BG544498_T1 b_rapa 2108 42 81 100 100 blastp 762 b_rapa|gb162|BQ791962_T2 b_rapa 2109 42 81 100 99.2982456 blastp 763 b_rapa|gb162|BQ791962_T1 b_rapa 2110 42 81 100 99.2982456 blastp 764 b_rapa|gb162|EX065729_T1 b_rapa 2111 42 81 92.1146953 100 blastp 765 b_rapa|gb162|CA992278_T1 b_rapa 2112 42 83 89.9641577 98.8372093 blastp 766 b_rapa|gb162|CO749284_T1 b_rapa 2113 42 81 100 99.2982456 blastp 767 barley|gb157.3|BE412486_T1 barley 2114 42 81 100 100 blastp 768 bean|gb164|CB542746_T1 bean 2115 42 80 100 100 blastp 769 bean|gb164|CB280567_T1 bean 2116 42 86 99.6415771 98.9473684 blastp 770 bean|gb164|BQ481649_T1 bean 2117 42 82 100 100 blastp 771 bean|gb164|CV532291_T1 bean 2118 42 86 99.6415771 98.9547038 blastp 772 brachypodium|gb161.xeno| brachypodium 2119 42 80 94.9820789 100 blastp BE416137_T1 773 brachypodium|gb161.xeno| brachypodium 2120 42 80 100 100 blastp AF139814_T1 774 canola|gb161|CN729066_T1 canola 2121 42 81 100 99.3031359 blastp 775 canola|gb161|DQ068169_T1 canola 2122 42 81 100 99.2982456 blastp 776 canola|gb161|AF118382_T1 canola 2123 42 81 100 99.3031359 blastp 777 canola|gb161|AF118383_T1 canola 2124 42 81 100 100 blastp 778 canola|gb161|CD819509_T1 canola 2125 42 80 100 99.2982456 blastp 779 canola|gb161|EE419467_T1 canola 2126 42 81 100 100 blastp 780 canola|gb161|EE459735_T1 canola 2127 42 81 100 99.2982456 blastp 781 canola|gb161|CX192356_T1 canola 2128 42 80 100 100 blastp 782 canola|gb161|CN827413_T1 canola 2129 42 81 100 99.2982456 blastp 783 canola|gb161|EE432011_T1 canola 2130 42 81 100 99.2982456 blastp 784 cassava|gb164|DB922106_T1 cassava 2131 42 81 93.90681 92.5266904 blastp 785 cassava|gb164|CK640888_T1 cassava 2132 42 83 100 100 blastp 786 cassava|gb164|CK642866_T1 cassava 2133 42 84 99.6415771 98.951049 blastp 787 cassava|gb164|CK642551_T1 cassava 2134 42 86 100 99.3055556 blastp 788 castorbean|gb160| castorbean 2135 42 86 100 99.3055556 blastp AJ605565_T1 789 castorbean|gb160| castorbean 2136 42 87 99.6415771 98.9399293 blastp AJ605568_T1 790 castorbean|gb160| castorbean 2137 42 85 100 100 blastp EE259660_T1 791 centaurea|gb161| centaurea 2138 42 84 87.0967742 92.8571429 blastp EL933765_T1 792 centaurea|gb161| centaurea 2139 42 82 75.9856631 89.2561983 blastp EL931761_T1 793 cichorium|gb161| cichorium 2140 42 86 100 100 blastp DT212328_T1 794 citrus|gb157.2|BQ625054_T1 citrus 2141 42 83 100 100 blastp 795 citrus|gb157.2|CF509045_T1 citrus 2142 42 86 100 99.3031359 blastp 796 citrus|gb157.2|CB291797_T1 citrus 2143 42 80 100 84.0707965 blastp 797 citrus|gb157.2|BQ623325_T1 citrus 2144 42 86 100 99.3031359 blastp 798 citrus|gb157.2|BQ623742_T1 citrus 2145 42 88 94.9820789 99.2619926 blastp 799 citrus|gb157.2|CF417769_T1 citrus 2146 42 83 100 99.3031359 blastp 800 citrus|gb157.2|BQ623128_T1 citrus 2147 42 88 68.4587814 98.9637306 blastp 801 citrus|gb157.2|CB293000_T1 citrus 2148 42 82 93.1899642 98.8847584 blastp 802 citrus|gb157.2|BQ622991_T1 citrus 2149 42 84 96.4157706 98.5559567 blastp 803 citrus|gb157.2|CF503882_T1 citrus 2150 42 83 100 100 blastp 804 cotton|gb164|BE052942_T1 cotton 2151 42 84 99.2831541 98.5815603 blastp 805 cotton|gb164|AF064467_T1 cotton 2152 42 80 100 100 blastp 806 cotton|gb164|BG443494_T1 cotton 2153 42 85 100 99.2982456 blastp 807 cotton|gb164|CO086106_T1 cotton 2154 42 88 70.2508961 100 blastp 808 cotton|gb164|BQ406033_T1 cotton 2155 42 82 100 99.2982456 blastp 809 cotton|gb164|CO109551_T1 cotton 2156 42 82 100 100 blastp 810 cotton|gb164|DV437970_T1 cotton 2157 42 80 64.1577061 95.3608247 blastp 811 cotton|gb164|AI725803_T1 cotton 2158 42 84 100 99.2982456 blastp 812 cotton|gb164|CD486305_T1 cotton 2159 42 81 98.9247312 94.0199336 blastp 813 cowpea|gb166|ES884222_T2 cowpea 2160 42 85 86.3799283 93.5606061 blastp 814 cowpea|gb166|ES884222_T1 cowpea 2161 42 86 99.6415771 98.9547038 blastp 815 cowpea|gb166|FF538675_T1 cowpea 2162 42 89 52.688172 98 blastp 816 cowpea|gb166|FC458151_T1 cowpea 2163 42 80 100 100 blastp 817 cowpea|gb166|FC458381_T1 cowpea 2164 42 85 99.6415771 98.9473684 blastp 818 cryptomeria|gb166| cryptomeria 2165 42 83 95.6989247 93.639576 blastp AU036821_T1 819 cryptomeria|gb166| cryptomeria 2166 42 81 53.046595 98.013245 blastp BW995927_T1 820 dandelion|gb161| dandelion 2167 42 87 93.90681 88.9632107 blastp DY827637_T1 821 dandelion|gb161| dandelion 2168 42 85 93.5483871 93.2862191 blastp DY814583_T1 822 dandelion|gb161| dandelion 2169 42 85 100 100 blastp DY828216_T1 823 dandelion|gb161| dandelion 2170 42 80 85.3046595 98.7654321 blastp DY818322_T1 824 dandelion|gb161| dandelion 2171 42 82 98.9247312 98.245614 blastp DY805523_T1 825 fescue|gb161|DT675934_T1 fescue 2172 42 82 61.2903226 93.9226519 blastp 826 ginger|gb164|DY345807_T1 ginger 2173 42 81 100 100 blastp 827 ginger|gb164|DY373920_T1 ginger 2174 42 88 69.5340502 100 blastp 828 grape|gb160|BQ792080_T1 grape 2175 42 81 100 99.3031359 blastp 829 grape|gb160|CB973593_T1 grape 2176 42 84 100 100 blastp 830 grape|gb160|BM437196_T1 grape 2177 42 84 100 99.2957746 blastp 831 iceplant|gb164|CIPMIPC_T1 iceplant 2178 42 83 100 100 blastp 832 iceplant|gb164|BE035661_T1 iceplant 2179 42 82 99.6415771 98.6254296 blastp 833 ipomoea|gb157.2| ipomoea 2180 42 80 99.6415771 100 blastp BM878800_T1 834 ipomoea|gb157.2| ipomoea 2181 42 90 86.7383513 93.2330827 blastp AU224434_T2 835 ipomoea|gb157.2| ipomoea 2182 42 82 100 99.2957746 blastp BJ553793_T1 836 ipomoea|gb157.2| ipomoea 2183 42 89 100 100 blastp AU224434_T1 837 lettuce|gb157.2| lettuce 2184 42 85 99.2831541 98.5964912 blastp DW115660_T1 838 lettuce|gb157.2| lettuce 2185 42 80 97.8494624 95.7894737 blastp DW113963_T1 839 lettuce|gb157.2| lettuce 2186 42 84 99.6415771 98.9399293 blastp DW110249_T1 840 lettuce|gb157.2| lettuce 2187 42 86 100 100 blastp DW051453_T1 841 lettuce|gb157.2| lettuce 2188 42 84 100 100 blastp DW076507_T1 842 lettuce|gb157.2| lettuce 2189 42 84 100 100 blastp DW127617_T1 843 lettuce|gb157.2| lettuce 2190 42 80 100 100 blastp AJ937963_T1 844 lettuce|gb157.2| lettuce 2191 42 83 92.8315412 98.5018727 blastp DW049421_T1 845 lettuce|gb157.2| lettuce 2192 42 87 99.2831541 98.9473684 blastp DW114305_T1 846 lettuce|gb157.2| lettuce 2193 42 80 100 100 blastp DW053722_T1 847 lettuce|gb157.2| lettuce 2194 42 81 100 100 blastp DW146178_T1 848 lettuce|gb157.2| lettuce 2195 42 85 99.2831541 98.5964912 blastp DW077710_T1 849 lettuce|gb157.2| lettuce 2196 42 84 100 100 blastp DW070566_T1 850 lettuce|gb157.2| lettuce 2197 42 85 94.9820789 100 blastp DW093078_T1 851 lettuce|gb157.2| lettuce 2198 42 84 97.4910394 96.1672474 blastp DW074446_T1 852 lettuce|gb157.2| lettuce 2199 42 83 99.6415771 98.9399293 blastp DW153482_T1 853 lettuce|gb157.2| lettuce 2200 42 86 100 100 blastp DW080306_T1 854 lettuce|gb157.2| lettuce 2201 42 84 99.6415771 98.9399293 blastp DW043674_T1 855 lettuce|gb157.2| lettuce 2202 42 83 97.1326165 98.5559567 blastp DW095979_T1 856 lettuce|gb157.2| lettuce 2203 42 84 100 100 blastp DW077206_T1 857 lettuce|gb157.2| lettuce 2204 42 86 98.9247312 99.6453901 blastp DW047573_T1 858 lettuce|gb157.2| lettuce 2205 42 87 100 100 blastp DW096304_T1 859 lettuce|gb157.2| lettuce 2206 42 81 100 100 blastp DW075191_T1 860 lotus|gb157.2|AI967757_T1 lotus 2207 42 85 99.6415771 98.9547038 blastp 861 lotus|gb157.2|AI967387_T2 lotus 2208 42 80 99.6415771 99.6515679 blastp 862 lotus|gb157.2|BG662315_T1 lotus 2209 42 82 99.6415771 98.9619377 blastp 863 maize|gb164|BE552783_T1 maize 2210 42 80 97.4910394 95.890411 blastp 864 maize|gb164|AI622334_T1 maize 2211 42 83 97.4910394 95.862069 blastp 865 maize|gb164|AI855280_T1 maize 2212 42 81 96.7741935 95.1557093 blastp 866 medicago|gb157.2| medicago 2213 42 80 100 99.3031359 blastp AW981259_T1 867 medicago|gb157.2| medicago 2214 42 83 99.6415771 98.9547038 blastp AA660788_T1 868 melon|gb165|DV631824_T1 melon 2215 42 84 100 100 blastp 869 melon|gb165|DV633977_T1 melon 2216 42 83 64.516129 100 blastp 870 melon|gb165|AM720039_T1 melon 2217 42 81 86.0215054 100 blastp 871 nicotiana_benthamiana| nicotiana_benthamiana 2218 42 80 100 100 blastp gb162|CN743200_T1 872 nicotiana_benthamiana| nicotiana_benthamiana 2219 42 80 98.2078853 96.8641115 blastp gb162|CK294539_T1 873 onion|gb162|AF255795_T1 onion 2220 42 82 97.1326165 95.532646 blastp 874 onion|gb162|CF434704_T1 onion 2221 42 80 99.2831541 99.2957746 blastp 875 papaya|gb165|EL784273_T1 papaya 2222 42 82 82.078853 97.5206612 blastp 876 papaya|gb165|AM904340_T1 papaya 2223 42 84 100 99.2882562 blastp 877 peach|gb157.2|AF367458_T1 peach 2224 42 84 87.0967742 100 blastp 878 peach|gb157.2|BU040116_T1 peach 2225 42 83 99.6415771 98.9547038 blastp 879 peach|gb157.2|AF367460_T1 peach 2226 42 86 87.0967742 100 blastp 880 peanut|gb161|CD037924_T1 peanut 2227 42 86 99.6415771 98.9547038 blastp 881 peanut|gb161|CD038296_T1 peanut 2228 42 85 99.6415771 98.9547038 blastp 882 peanut|gb161|CD037924_T2 peanut 2229 42 86 99.6415771 98.9547038 blastp 883 peanut|gb161|CD037884_T1 peanut 2230 42 88 51.2544803 97.9452055 blastp 884 pepper|gb157.2| pepper 2231 42 96 100 100 blastp BM061612_T1 885 pepper|gb157.2| pepper 2232 42 97 100 100 blastp BM061611_T1 886 pepper|gb157.2| pepper 2233 42 81 92.4731183 97.3977695 blastp BM061005_T1 887 petunia|gb157.2| petunia 2234 42 83 97.4910394 98.2332155 blastp AF452014_T1 888 petunia|gb157.2| petunia 2235 42 95 53.046595 93.6708861 blastp CV295523_T1 889 petunia|gb157.2| petunia 2236 42 83 55.5555556 100 blastp CV293001_T1 890 pine|gb157.2|AI813221_T1 pine 2237 42 81 96.0573477 94.3262411 blastp 891 pine|gb157.2|CA844411_T1 pine 2238 42 81 56.6308244 98.75 blastp 892 poplar|gb157.2|BI130501_T3 poplar 2239 42 85 100 99.2982456 blastp 893 poplar|gb157.2|AI165755_T1 poplar 2240 42 86 99.6415771 98.9473684 blastp 894 poplar|gb157.2|BU835712_T1 poplar 2241 42 85 99.6415771 98.9473684 blastp 895 poplar|gb157.2|BI130501_T1 poplar 2242 42 85 100 99.2982456 blastp 896 poplar|gb157.2|BI130501_T4 poplar 2243 42 85 100 99.2982456 blastp 897 poplar|gb157.2|AI162288_T1 poplar 2244 42 86 99.2831541 98.5964912 blastp 898 poplar|gb157.2|AJ534524_T1 poplar 2245 42 84 100 100 blastp 899 potato|gb157.2|BG096672_T1 potato 2246 42 96 100 100 blastp 900 potato|gb157.2|BM109370_T1 potato 2247 42 80 98.2078853 96.8641115 blastp 901 potato|gb157.2|BG589618_T1 potato 2248 42 80 98.2078853 96.8641115 blastp 902 potato|gb157.2|CK261080_T1 potato 2249 42 83 70.2508961 100 blastp 903 potato|gb157.2|BG098124_T1 potato 2250 42 97 99.2831541 100 blastp 904 potato|gb157.2|BI406400_T1 potato 2251 42 80 98.2078853 96.8641115 blastp 905 potato|gb157.2|BE920139_T1 potato 2252 42 90 100 100 blastp 906 potato|gb157.2|BM112017_T1 potato 2253 42 80 98.2078853 96.8641115 blastp 907 potato|gb157.2|BE921679_T1 potato 2254 42 96 100 100 blastp 908 potato|gb157.2|BG600158_T1 potato 2255 42 80 98.2078853 96.8641115 blastp 909 potato|gb157.2|BI406047_T1 potato 2256 42 80 100 100 blastp 910 potato|gb157.2|CK719282_T1 potato 2257 42 80 98.2078853 96.8641115 blastp 911 radish|gb164|EV545956_T1 radish 2258 42 81 100 99.2982456 blastp 912 radish|gb164|EX904869_T1 radish 2259 42 80 100 100 blastp 913 radish|gb164|EV545247_T1 radish 2260 42 80 100 100 blastp 914 radish|gb164|EX756889_T1 radish 2261 42 80 100 100 blastp 915 radish|gb164|EV539533_T1 radish 2262 42 81 100 99.3031359 blastp 916 radish|gb164|EV546186_T1 radish 2263 42 82 55.5555556 95.6790123 blastp 917 radish|gb164|AB012045_T1 radish 2264 42 81 100 99.3031359 blastp 918 radish|gb164|EV573001_T1 radish 2265 42 83 62.0071685 98.8571429 blastp 919 radish|gb164|AB030698_T1 radish 2266 42 81 100 100 blastp 920 radish|gb164|EX749049_T1 radish 2267 42 83 78.4946237 100 blastp 921 radish|gb164|AB030697_T1 radish 2268 42 80 100 100 blastp 922 radish|gb164|EW735060_T1 radish 2269 42 80 96.4157706 95.4545455 blastp 923 radish|gb164|FD936119_T1 radish 2270 42 83 50.5376344 100 blastp 924 radish|gb164|EV543747_T1 radish 2271 42 81 100 99.3031359 blastp 925 rice|gb157.2|BE040651_T2 rice 2272 42 80 86.7383513 94.3820225 blastp 926 rice|gb157.2|BE040651_T1 rice 2273 42 80 100 100 blastp 927 rice|gb157.2|AA754435_T5 rice 2274 42 80 85.6630824 87.7192982 blastp 928 rice|gb157.2|AA754435_T1 rice 2275 42 81 100 100 blastp 929 rose|gb157.2|BI977420_T1 rose 2276 42 84 69.8924731 91.627907 blastp 930 rose|gb157.2|BI977750_T1 rose 2277 42 84 77.7777778 100 blastp 931 rose|gb157.2|BI978110_T1 rose 2278 42 83 64.516129 98.3783784 blastp 932 safflower|gb162| safflower 2279 42 81 98.5663082 100 blastp EL406648_T1 933 safflower|gb162| safflower 2280 42 86 92.4731183 97.761194 blastp EL411110_T1 934 safflower|gb162| safflower 2281 42 85 100 100 blastp EL401452_T1 935 safflower|gb162| safflower 2282 42 83 97.1326165 100 blastp EL402424_T1 936 safflower|gb162| safflower 2283 42 85 86.7383513 67.9193401 tblastn EL400227_T1 937 sorghum|gb161.xeno| sorghum 2284 42 83 97.4910394 95.862069 blastp AI622334_T1 938 soybean|gb166|CD393286_T1 soybean 2285 42 80 99.6415771 99.6515679 blastp 939 soybean|gb166|GMU27347_T1 soybean 2286 42 86 99.6415771 98.9473684 blastp 940 soybean|gb166|AW349289_T1 soybean 2287 42 85 99.6415771 98.9473684 blastp 941 soybean|gb166|BE823946_T1 soybean 2288 42 87 99.6415771 98.943662 blastp 942 soybean|gb166|BE352729_T2 soybean 2289 42 80 56.2724014 100 blastp 943 soybean|gb166|AW350475_T1 soybean 2290 42 85 99.6415771 98.9547038 blastp 944 soybean|gb166|BI119558_T1 soybean 2291 42 80 100 100 blastp 945 soybean|gb166|AW349392_T1 soybean 2292 42 80 100 100 blastp 946 spruce|gb162|AF051202_T1 spruce 2293 42 81 96.0573477 94.3262411 blastp 947 spruce|gb162|CO227554_T1 spruce 2294 42 80 96.0573477 93.6619718 blastp 948 spruce|gb162|CO220480_T1 spruce 2295 42 80 96.4157706 94.6808511 blastp 949 spruce|gb162|CO217151_T1 spruce 2296 42 80 96.0573477 94.3262411 blastp 950 spurge|gb161|AW990929_T1 spurge 2297 42 80 59.8566308 91.5343915 blastp 951 spurge|gb161|BG354126_T1 spurge 2298 42 83 100 100 blastp 952 spurge|gb161|DV125170_T1 spurge 2299 42 87 83.5125448 99.5780591 blastp 953 strawberry|gb164| strawberry 2300 42 85 99.6415771 98.9473684 blastp DV438166_T1 954 strawberry|gb164| strawberry 2301 42 86 100 99.2957746 blastp EX665494_T1 955 strawberry|gb164| strawberry 2302 42 85 100 100 blastp CO817390_T1 956 sugarcane|gb157.2| sugarcane 2303 42 85 70.2508961 100 blastp BQ536871_T2 957 sugarcane|gb157.2| sugarcane 2304 42 83 97.4910394 92.6666667 blastp BU103568_T1 958 sugarcane|gb157.2| sugarcane 2305 42 81 97.4910394 95.862069 blastp BQ536871_T1 959 sugarcane|gb157.2| sugarcane 2306 42 84 97.4910394 95.862069 blastp BQ535332_T1 960 sunflower|gb162| sunflower 2307 42 83 100 100 blastp CD849689_T1 961 sunflower|gb162| sunflower 2308 42 83 94.9820789 95.0704225 blastp CD849494_T1 962 sunflower|gb162| sunflower 2309 42 81 83.5125448 98.75 blastp CF091932_T1 963 sunflower|gb162| sunflower 2310 42 81 100 100 blastp DY915644_T1 964 sunflower|gb162| sunflower 2311 42 81 78.4946237 88.8446215 blastp EL462160_T1 965 sunflower|gb162| sunflower 2312 42 87 95.6989247 100 blastp DY918039_T1 966 sunflower|gb162| sunflower 2313 42 87 89.9641577 95.5223881 blastp DY951506_T1 967 sunflower|gb162| sunflower 2314 42 84 99.6415771 98.9473684 blastp DY933519_T1 968 sunflower|gb162| sunflower 2315 42 82 100 100 blastp DY917102_T1 969 sunflower|gb162| sunflower 2316 42 86 58.0645161 100 blastp DY932916_T1 970 sunflower|gb162| sunflower 2317 42 84 100 100 blastp CD857425_T1 971 sunflower|gb162| sunflower 2318 42 85 53.4050179 97.4683544 blastp CD846314_T1 972 sunflower|gb162| sunflower 2319 42 88 68.1003584 89.6226415 blastp CX944368_T1 973 sunflower|gb162| sunflower 2320 42 80 100 100 blastp DY908592_T1 974 switchgrass|gb165| switchgrass 2321 42 81 96.7741935 98.9208633 blastp DN141399_T1 975 switchgrass|gb165| switchgrass 2322 42 86 51.9713262 99.3150685 blastp FE621421_T1 976 switchgrass|gb165| switchgrass 2323 42 83 97.4910394 95.862069 blastp DN145656_T1 977 switchgrass|gb165| switchgrass 2324 42 81 82.437276 100 blastp FE637674_T1 978 switchgrass|gb165| switchgrass 2325 42 83 77.0609319 94.8497854 blastp DN141334_T1 979 switchgrass|gb165| switchgrass 2326 42 83 85.6630824 100 blastp FE621578_T1 980 thellungiella|gb157.2| thellungiella 2327 42 81 72.4014337 100 blastp DN775526_T1 981 tobacco|gb162|CK720599_T1 tobacco 2328 42 95 100 100 blastp 982 tobacco|gb162|CK720599_T2 tobacco 2329 42 94 100 100 blastp 983 tobacco|gb162|EB445427_T1 tobacco 2330 42 81 100 100 blastp 984 tobacco|gb162|EB443112_T1 tobacco 2331 42 89 100 100 blastp 985 tobacco|gb162|AF154641_T1 tobacco 2332 42 80 100 100 blastp 986 tobacco|gb162|EB425288_T1 tobacco 2333 42 94 98.2078853 100 blastp 987 tomato|gb164|BG123951_T2 tomato 2334 42 81 92.8315412 97.4074074 blastp 988 tomato|gb164|BG123951_T1 tomato 2335 42 80 98.2078853 96.8641115 blastp 989 tomato|gb164|BG713781_T1 tomato 2336 42 92 53.4050179 98.0519481 blastp 990 tomato|gb164|AW219533_T1 tomato 2337 42 81 97.1326165 98.2142857 blastp 991 triphysaria|gb164| triphysaria 2338 42 88 99.2831541 99.2857143 blastp DR170852_T1 992 triphysaria|gb164| triphysaria 2339 42 88 64.874552 100 blastp BM356582_T1 993 triphysaria|gb164| triphysaria 2340 42 80 100 100 blastp BE574767_T1 994 wheat|gb164|BE444481_T1 wheat 2341 42 82 55.5555556 100 blastp 995 wheat|gb164|BE398316_T1 wheat 2342 42 81 100 100 blastp 996 wheat|gb164|BE404002_T1 wheat 2343 42 82 51.6129032 99.3055556 blastp 997 apple|gb157.3|CN892655_T1 apple 2344 43 84 100 100 blastp 998 apple|gb157.3|AU223658_T1 apple 2345 43 85 100 100 blastp 999 aquilegia|gb157.3| aquilegia 2346 43 84 100 100 blastp DR914359_T1 1000 arabidopsis|gb165| arabidopsis 2347 43 84 100 100 blastp AT2G16850_T1 1001 arabidopsis|gb165| arabidopsis 2348 43 86 100 100 blastp AT4G35100_T1 1002 avocado|gb164|CK753629_T1 avocado 2349 43 85 66.4310954 92.6108374 blastp 1003 avocado|gb164|CK752541_T1 avocado 2350 43 84 62.1908127 100 blastp 1004 b_juncea|gb164| b_juncea 2351 43 86 67.1378092 100 blastp EVGN01060535361904_T1 1005 b_juncea|gb164| b_juncea 2352 43 86 85.1590106 100 blastp EVGN00138211060167_T1 1006 b_juncea|gb164| b_juncea 2353 43 86 66.0777385 100 blastp EVGN01177009332048_T1 1007 b_juncea|gb164| b_juncea 2354 43 86 90.459364 100 blastp EVGN00071418640425_T1 1008 b_juncea|gb164| b_juncea 2355 43 92 53.0035336 100 blastp EVGN01391814101746_T1 1009 b_juncea|gb164| b_juncea 2356 43 85 100 100 blastp EVGN00206114600060_T1 1010 b_oleracea|gb161| b_oleracea 2357 43 85 100 100 blastp AF314656_T1 1011 b_oleracea|gb161| b_oleracea 2358 43 86 100 100 blastp DY029187_T1 1012 b_oleracea|gb161| b_oleracea 2359 43 87 54.770318 100 blastp DY014978_T1 1013 b_rapa|gb162|BQ791230_T1 b_rapa 2360 43 86 77.0318021 97.7375566 blastp 1014 b_rapa|gb162|EX033370_T1 b_rapa 2361 43 87 100 100 blastp 1015 b_rapa|gb162|CV432651_T1 b_rapa 2362 43 86 100 100 blastp 1016 b_rapa|gb162|BG543906_T1 b_rapa 2363 43 85 100 100 blastp 1017 bean|gb164|CB539790_T1 bean 2364 43 83 100 100 blastp 1018 beet|gb162|BVU60148_T1 beet 2365 43 80 100 100 blastp 1019 canola|gb161|DY007064_T1 canola 2366 43 86 100 100 blastp 1020 canola|gb161|CD815284_T1 canola 2367 43 85 100 100 blastp 1021 canola|gb161|CD817684_T1 canola 2368 43 85 100 100 blastp 1022 canola|gb161|CD821191_T1 canola 2369 43 86 100 100 blastp 1023 canola|gb161|CD820375_T1 canola 2370 43 87 100 100 blastp 1024 cassava|gb164|BM259748_T1 cassava 2371 43 84 100 100 blastp 1025 castorbean|gb160| castorbean 2372 43 84 100 100 blastp AJ605569_T1 1026 castorbean|gb160| castorbean 2373 43 84 69.9646643 95.1219512 blastp AJ605569_T2 1027 cichorium|gb161| cichorium 2374 43 89 67.1378092 100 blastp EH704748_T1 1028 citrus|gb157.2|BQ623127_T1 citrus 2375 43 85 100 100 blastp 1029 citrus|gb157.2|CX664964_T1 citrus 2376 43 84 100 100 blastp 1030 clover|gb162|BB911260_T1 clover 2377 43 82 54.0636042 100 blastp 1031 coffea|gb157.2|BQ448890_T1 coffea 2378 43 86 100 100 blastp 1032 cotton|gb164|AI726086_T1 cotton 2379 43 84 97.8798587 88.02589 blastp 1033 cotton|gb164|CO098535_T1 cotton 2380 43 82 92.2261484 95.5719557 blastp 1034 cotton|gb164|BE051956_T1 cotton 2381 43 83 100 100 blastp 1035 cotton|gb164|BQ414250_T1 cotton 2382 43 85 59.0106007 100 blastp 1036 cotton|gb164|BF268907_T1 cotton 2383 43 87 90.1060071 100 blastp 1037 cotton|gb164|CO075847_T1 cotton 2384 43 84 62.5441696 100 blastp 1038 cotton|gb164|BQ405584_T1 cotton 2385 43 82 95.4063604 95.7142857 blastp 1039 cotton|gb164|AI055551_T1 cotton 2386 43 85 100 100 blastp 1040 cowpea|gb166|FC456786_T1 cowpea 2387 43 84 100 100 blastp 1041 dandelion|gb161| dandelion 2388 43 85 100 100 blastp DY803814_T1 1042 ginger|gb164|DY347270_T1 ginger 2389 43 80 100 100 blastp 1043 ginger|gb164|DY347296_T1 ginger 2390 43 84 94.6996466 92.733564 blastp 1044 ginger|gb164|DY358056_T1 ginger 2391 43 81 100 100 blastp 1045 ginger|gb164|DY347277_T1 ginger 2392 43 81 100 100 blastp 1046 ginger|gb164|DY360032_T1 ginger 2393 43 81 81.9787986 93.902439 blastp 1047 grape|gb160|BM437910_T1 grape 2394 43 84 100 100 blastp 1048 iceplant|gb164| iceplant 2395 43 82 100 100 blastp MCU26538_T1 1049 ipomoea|gb157.2| ipomoea 2396 43 83 100 100 blastp BJ553491_T1 1050 lettuce|gb157.2| lettuce 2397 43 84 100 100 blastp DW046509_T1 1051 lettuce|gb157.2| lettuce 2398 43 84 97.8798587 100 blastp DW106553_T1 1052 lettuce|gb157.2| lettuce 2399 43 84 100 100 blastp DW146059_T1 1053 lettuce|gb157.2| lettuce 2400 43 83 97.1731449 100 blastp DW110223_T1 1054 lettuce|gb157.2| lettuce 2401 43 84 100 100 blastp DW079482_T1 1055 lettuce|gb157.2| lettuce 2402 43 83 97.5265018 92.8327645 blastp DW094572_T1 1056 lotus|gb157.2|AW163949_T1 lotus 2403 43 85 54.4169611 93.902439 blastp 1057 lotus|gb157.2|AI967574_T1 lotus 2404 43 82 98.5865724 97.5352113 blastp 1058 melon|gb165|DV632217_T1 melon 2405 43 83 100 100 blastp 1059 oil_palm|gb166| oil_palm 2406 43 80 100 100 blastp CN600073_T1 1060 papaya|gb165|EX227970_T1 papaya 2407 43 85 100 100 blastp 1061 peach|gb157.2|AF367457_T1 peach 2408 43 85 85.1590106 99.5833333 blastp 1062 pepper|gb157.2| pepper 2409 43 96 67.4911661 98.9637306 blastp BM066074_T1 1063 pepper|gb157.2| pepper 2410 43 88 53.7102473 100 blastp CA518686_T1 1064 periwinkle|gb164| periwinkle 2411 43 90 100 100 blastp AM232518_T1 1065 petunia|gb157.2| petunia 2412 43 89 100 100 blastp AF452013_T1 1066 poplar|gb157.2|AI163573_T1 poplar 2413 43 84 100 100 blastp 1067 poplar|gb157.2|AI162424_T1 poplar 2414 43 83 100 100 blastp 1068 potato|gb157.2|BF053675_T1 potato 2415 43 89 83.745583 100 blastp 1069 potato|gb157.2|BF459952_T1 potato 2416 43 97 100 100 blastp 1070 radish|gb164|EV526465_T1 radish 2417 43 86 100 100 blastp 1071 radish|gb164|EV539317_T1 radish 2418 43 85 100 100 blastp 1072 radish|gb164|EV525026_T1 radish 2419 43 85 100 100 blastp 1073 rose|gb157.2|BQ103996_T1 rose 2420 43 82 100 100 blastp 1074 safflower|gb162| safflower 2421 43 83 100 100 blastp EL372747_T1 1075 sesame|gb157.2| sesame 2422 43 88 50.8833922 100 blastp BU669158_T1 1076 sesame|gb157.2| sesame 2423 43 87 51.9434629 100 blastp BU668646_T1 1077 soybean|gb166|BE823128_T1 soybean 2424 43 82 100 100 blastp 1078 soybean|gb166|BE352716_T1 soybean 2425 43 82 100 100 blastp 1079 spruce|gb162|CO217407_T1 spruce 2426 43 80 93.2862191 93.5714286 blastp 1080 spurge|gb161|AW821924_T1 spurge 2427 43 84 100 97.2125436 blastp 1081 strawberry|gb164| strawberry 2428 43 82 100 100 blastp CX661107_T1 1082 sunflower|gb162| sunflower 2429 43 85 100 100 blastp CD853582_T1 1083 sunflower|gb162| sunflower 2430 43 80 97.1731449 97.833935 blastp DY939653_T1 1084 sunflower|gb162| sunflower 2431 43 81 100 100 blastp CD849663_T1 1085 thellungiella|gb157.2| thellungiella 2432 43 86 72.0848057 90.5829596 blastp DN777165_T1 1086 tobacco|gb162|CK720587_T1 tobacco 2433 43 90 99.6466431 100 blastp 1087 tobacco|gb162|CK720585_T1 tobacco 2434 43 90 100 100 blastp 1088 tobacco|gb162|CV016422_T1 tobacco 2435 43 96 59.7173145 100 blastp 1089 tobacco|gb162|CK720589_T1 tobacco 2436 43 94 100 100 blastp 1090 tomato|gb164|BG124140_T1 tomato 2437 43 88 100 100 blastp 1091 triphysaria|gb164| triphysaria 2438 43 83 100 100 blastp EY018490_T1 1092 triphysaria|gb164| triphysaria 2439 43 84 100 100 blastp EY007858_T1 1093 apple|gb157.3|CN494428_T1 apple 2440 44 80 79.1390728 93.0232558 blastp 1094 castorbean|gb160| castorbean 2441 44 82 99.0066225 97.7272727 blastp EG696741_T1 1095 citrus|gb157.2|CX074332_T1 citrus 2442 44 82 99.0066225 97.6973684 blastp 1096 citrus|gb157.2|CX674035_T1 citrus 2443 44 80 86.7549669 85.8085809 blastp 1097 cotton|gb164|DW234737_T1 cotton 2444 44 80 99.6688742 98.3333333 blastp 1098 lettuce|gb157.2| lettuce 2445 44 80 95.0331126 100 blastp DW066284_T1 1099 petunia|gb157.2| petunia 2446 44 84 65.8940397 100 blastp CV298254_T1 1100 potato|gb157.2|CV500020_T1 potato 2447 44 94 72.1854305 99.543379 blastp 1101 tobacco|gb162|EB426672_T1 tobacco 2448 44 89 99.0066225 97.689769 blastp 1102 brachypodium|gb161.xeno| brachypodium 2449 46 92 100 100 blastp BE415047_T1 1103 maize|gb164|CF244342_T1 maize 2450 46 90 90.7630522 100 blastp 1104 maize|gb164|AF057183_T1 maize 2451 46 89 100 100 blastp 1105 sorghum|gb161.xeno| sorghum 2452 46 88 100 100 blastp AF057183_T1 1106 sugarcane|gb157.2| sugarcane 2453 46 89 100 100 blastp CA132045_T1 1107 switchgrass|gb165| switchgrass 2454 46 90 100 100 blastp FE617713_T1 1108 wheat|gb164|BE430088_T1 wheat 2455 46 95 100 100 blastp 1109 apple|gb157.3|DT001281_T1 apple 2456 47 81 87.9518072 100 blastp 1110 brachypodium|gb161.xeno| brachypodium 2457 47 95 100 100 blastp BE402447_T1 1111 ginger|gb164|DY364894_T1 ginger 2458 47 88 64.2570281 98.7654321 blastp 1112 maize|gb164|AI855402_T1 maize 2459 47 89 100 100 blastp 1113 maize|gb164|AW017703_T1 maize 2460 47 85 100 100 blastp 1114 onion|gb162|ACU58207_T1 onion 2461 47 83 99.5983936 99.5967742 blastp 1115 onion|gb162|CF437464_T1 onion 2462 47 85 95.9839357 98.75 blastp 1116 onion|gb162|CF435351_T1 onion 2463 47 84 100 100 blastp 1117 rice|gb157.2|AU031632_T1 rice 2464 47 91 100 100 blastp 1118 rice|gb157.2|CA756239_T1 rice 2465 47 87 97.5903614 31.640625 blastp 1119 sorghum|gb161.xeno| sorghum 2466 47 89 100 100 blastp AI855402_T1 1120 sugarcane|gb157.2| sugarcane 2467 47 90 99.5983936 99.5967742 blastp CA132045_T2 1121 switchgrass|gb165| switchgrass 2468 47 90 100 100 blastp FE624217_T1 1122 wheat|gb164|BE404100_T1 wheat 2469 47 97 100 100 blastp 1123 fescue|gb161|DT700572_T1 fescue 2470 48 94 100 100 blastp 1124 ginger|gb164|DY361836_T1 ginger 2471 48 80 95.1612903 96.7346939 blastp 1125 rice|gb157.2|U37952_T1 rice 2472 48 90 100 100 blastp 1126 rice|gb157.2|U37952_T3 rice 2473 48 89 51.6129032 89.5104895 blastp 1127 rye|gb164|BE495605_T1 rye 2474 48 98 80.6451613 100 blastp 1128 sorghum|gb161.xeno| sorghum 2475 48 90 100 100 blastp AI724211_T1 1129 sugarcane|gb157.2| sugarcane 2476 48 82 92.3387097 100 blastp CA110414_T1 1130 sugarcane|gb157.2| sugarcane 2477 48 83 90.3225806 96.5517241 blastp CA065356_T1 1131 sugarcane|gb157.2| sugarcane 2478 48 83 71.3709677 95.6756757 blastp CA143208_T1 1132 sugarcane|gb157.2| sugarcane 2479 48 91 100 100 blastp CA101765_T1 1133 sugarcane|gb157.2| sugarcane 2480 48 90 83.4677419 95.8333333 blastp CA133231_T1 1134 switchgrass|gb165| switchgrass 2481 48 91 100 100 blastp FE605472_T1 1135 wheat|gb164|BE489764_T1 wheat 2482 48 95 100 100 blastp 1136 wheat|gb164|TAU86763_T1 wheat 2483 48 95 100 100 blastp 1137 wheat|gb164|BE415001_T1 wheat 2484 48 95 100 100 blastp 1138 b_juncea|gb164| b_juncea 2485 50 80 85.0931677 100 blastp EVGN00504508791211_T1 1139 b_juncea|gb164| b_juncea 2486 50 84 50.931677 91.954023 blastp EVGN01684214261870_T1 1140 banana|gb160|DN239388_T1 banana 2487 50 84 80.1242236 91.9708029 blastp 1141 barley|gb157.3|BF253694_T1 barley 2488 50 91 58.3850932 98.9690722 blastp 1142 barley|gb157.3|BE412959_T3 barley 2489 50 95 100 62.6923077 blastp 1143 bean|gb164|FD793482_T1 bean 2490 50 82 100 91.9075145 blastp 1144 canola|gb161|CX193398_T3 canola 2491 50 83 99.378882 66.9527897 blastp 1145 canola|gb161|EV123336_T1 canola 2492 50 81 52.7950311 91.2087912 blastp 1146 centaurea|gb161| centaurea 2493 50 81 98.136646 62.248996 blastp EL931277_T1 1147 cichorium|gb161| cichorium 2494 50 83 64.5962733 95.3271028 blastp DT213939_T1 1148 fescue|gb161|DT703843_T1 fescue 2495 50 99 100 94.1520468 blastp 1149 lotus|gb157.2|BI418499_T1 lotus 2496 50 86 98.136646 88.700565 blastp 1150 onion|gb162|BQ579939_T1 onion 2497 50 86 100 57.1942446 blastp 1151 peach|gb157.2|BU040795_T1 peach 2498 50 82 98.136646 56.2043796 blastp 1152 peach|gb157.2|DW351857_T1 peach 2499 50 83 98.136646 91.8128655 blastp 1153 rye|gb164|BF429463_T1 rye 2500 50 88 93.1677019 100 blastp 1154 soybean|gb166|BE352747_T4 soybean 2501 50 81 98.136646 70.7207207 blastp 1155 sugarcane|gb157.2| sugarcane 2502 50 81 98.136646 56.6787004 blastp CA194640_T1 1156 sugarcane|gb157.2| sugarcane 2503 50 84 96.2732919 87.0056497 blastp CA103332_T1 1157 sugarcane|gb157.2| sugarcane 2504 50 82 93.7888199 74.2574257 blastp CA167616_T1 1158 sugarcane|gb157.2| sugarcane 2505 50 90 100 68.5344828 blastp CA103740_T1 1159 sugarcane|gb157.2| sugarcane 2506 50 82 97.515528 77.4834437 tblastn CA184547_T1 1160 sunflower|gb162| sunflower 2507 50 87 98.136646 87.5 blastp CF089373_T1 1161 wheat|gb164|CA484201_T1 wheat 2508 50 89 96.8944099 65.9574468 blastp 1162 apricot|gb157.2| apricot 2509 51 86 100 65.9574468 blastp CV049856_T1 1163 arabidopsis|gb165| arabidopsis 2510 51 89 100 32.6315789 blastp AT2G37180_T1 1164 arabidopsis|gb165| arabidopsis 2511 51 92 100 32.1799308 blastp AT2G39010_T1 1165 b_juncea|gb164| b_juncea 2512 51 87 100 86.9158879 blastp EVGN00130608921231_T1 1166 b_juncea|gb164| b_juncea 2513 51 86 90.3225806 86.5979381 blastp EVGN00605203140273_T1 1167 b_juncea|gb164| b_juncea 2514 51 86 56.9892473 91.3793103 blastp EVGN21262514941904_T1 1168 b_juncea|gb164| b_juncea 2515 51 86 100 85.3211009 blastp EVGN00733314152324_T1 1169 b_juncea|gb164| b_juncea 2516 51 86 89.2473118 49.4047619 blastp EVGN00041211340240_T1 1170 b_juncea|gb164| b_juncea 2517 51 91 100 80.1724138 blastp DT317704_T1 1171 b_juncea|gb164| b_juncea 2518 51 85 89.2473118 79.8076923 blastp EVGN00208014701957_T1 1172 b_juncea|gb164| b_juncea 2519 51 90 100 36.328125 blastp EVGN00550314491066_T1 1173 b_juncea|gb164| b_juncea 2520 51 90 90.3225806 95.4545455 blastp EVGN01267508262672_T1 1174 b_juncea|gb164| b_juncea 2521 51 89 100 51.3812155 blastp EVGN01803715320789_T1 1175 b_juncea|gb164| b_juncea 2522 51 91 100 34.4019729 tblastn EVGN00397011681539_T1 1176 b_rapa|gb162|DN965016_T1 b_rapa 2523 51 88 100 69.4029851 blastp 1177 b_rapa|gb162|EX025548_T1 b_rapa 2524 51 87 100 32.5174825 blastp 1178 b_rapa|gb162|BG543764_T1 b_rapa 2525 51 92 100 32.2916667 blastp 1179 banana|gb160|DN238638_T1 banana 2526 51 89 100 27.8721279 tblastn 1180 banana|gb160|DN238638_T2 banana 2527 51 89 100 30.5921053 tblastn 1181 barley|gb157.3|BE421292_T1 barley 2528 51 87 100 32.7464789 blastp 1182 barley|gb157.3|BJ446923_T1 barley 2529 51 83 100 64.5833333 blastp 1183 beet|gb162|BQ488455_T1 beet 2530 51 81 100 26.686217 blastp 1184 beet|gb162|BVU60147_T1 beet 2531 51 84 100 32.2916667 blastp 1185 canola|gb161|CX189721_T1 canola 2532 51 87 100 32.5174825 blastp 1186 canola|gb161|CB686155_T1 canola 2533 51 92 100 32.2916667 blastp 1187 canola|gb161|DY007249_T1 canola 2534 51 87 100 32.5174825 blastp 1188 canola|gb161|ES986486_T1 canola 2535 51 86 96.7741935 87.3786408 blastp 1189 canola|gb161|EV203446_T1 canola 2536 51 82 100 38.0108992 tblastn 1190 cassava|gb164|DN740353_T1 cassava 2537 51 93 100 62.8378378 blastp 1191 cassava|gb164|DV449516_T1 cassava 2538 51 94 100 68.8888889 blastp 1192 cassava|gb164|BM259717_T1 cassava 2539 51 81 100 32.8621908 blastp 1193 castorbean|gb160| castorbean 2540 51 81 100 46.5 blastp EE257493_T2 1194 castorbean|gb160| castorbean 2541 51 81 100 34.4444444 blastp EE257493_T1 1195 cichorium|gb161| cichorium 2542 51 94 100 80.8695652 blastp EH706421_T1 1196 cichorium|gb161| cichorium 2543 51 90 100 32.5554259 tblastn EH708948_T1 1197 cichorium|gb161| cichorium 2544 51 90 100 24.668435 tblastn EH692078_T1 1198 citrus|gb157.2|CB291468_T1 citrus 2545 51 82 100 86.1111111 blastp 1199 citrus|gb157.2|BQ624699_T1 citrus 2546 51 90 100 27.56917 tblastn 1200 clover|gb162|BB903718_T1 clover 2547 51 91 100 32.6315789 blastp 1201 clover|gb162|BB930902_T1 clover 2548 51 88 100 32.4041812 blastp 1202 clover|gb162|BB911526_T1 clover 2549 51 91 92.4731183 80.3738318 blastp 1203 clover|gb162|BB913405_T1 clover 2550 51 90 96.7741935 81.0810811 blastp 1204 cotton|gb164|ES804497_T1 cotton 2551 51 86 100 58.490566 blastp 1205 cotton|gb164|EX172153_T1 cotton 2552 51 89 90.3225806 41.5156507 tblastn 1206 cowpea|gb166|FF384339_T1 cowpea 2553 51 89 90.3225806 80 blastp 1207 cowpea|gb166|FF396241_T1 cowpea 2554 51 82 97.8494624 88.3495146 blastp 1208 cowpea|gb166|ES884224_T1 cowpea 2555 51 88 100 32.4041812 blastp 1209 cowpea|gb166|FG857474_T1 cowpea 2556 51 89 100 68.8888889 blastp 1210 cryptomeria|gb166| cryptomeria 2557 51 81 100 78.8135593 blastp BY902595_T1 1211 cryptomeria|gb166| cryptomeria 2558 51 83 100 31.7406143 blastp BJ937695_T1 1212 cryptomeria|gb166| cryptomeria 2559 51 82 88.172043 86.3157895 blastp BW993227_T1 1213 dandelion|gb161| dandelion 2560 51 87 91.3978495 75.2212389 blastp DY802675_T1 1214 fescue|gb161|DT674412_T1 fescue 2561 51 82 84.9462366 85.8695652 blastp 1215 fescue|gb161|DT695652_T1 fescue 2562 51 84 100 76.8595041 blastp 1216 fescue|gb161|DT702501_T1 fescue 2563 51 83 95.6989247 96.7391304 blastp 1217 fescue|gb161|DT688112_T1 fescue 2564 51 83 100 85.3211009 blastp 1218 fescue|gb161|DT688728_T1 fescue 2565 51 92 100 81.5789474 blastp 1219 ginger|gb164|DY358169_T1 ginger 2566 51 87 100 32.9787234 blastp 1220 ginger|gb164|DY366672_T1 ginger 2567 51 82 93.5483871 87 blastp 1221 ginger|gb164|DY360033_T1 ginger 2568 51 91 100 27.7888446 tblastn 1222 grape|gb160|AF188843_T5 grape 2569 51 82 100 32.5174825 blastp 1223 ipomoea|gb157.2| ipomoea 2570 51 88 100 32.4041812 blastp BJ556470_T1 1224 lettuce|gb157.2| lettuce 2571 51 90 100 32.6315789 blastp DW158018_T1 1225 lettuce|gb157.2| lettuce 2572 51 90 100 34.7014925 blastp DW091407_T1 1226 lettuce|gb157.2| lettuce 2573 51 89 89.2473118 32.6771654 blastp DW060777_T1 1227 lotus|gb157.2|AV775277_T1 lotus 2574 51 83 100 88.5714286 blastp 1228 lotus|gb157.2|AI967387_T1 lotus 2575 51 84 100 32.4041812 blastp 1229 lotus|gb157.2|AV774377_T1 lotus 2576 51 81 100 88.5714286 blastp 1230 lotus|gb157.2|BP059122_T1 lotus 2577 51 80 73.1182796 85 blastp 1231 lotus|gb157.2|BI419853_T1 lotus 2578 51 81 94.6236559 88 blastp 1232 lotus|gb157.2|BP049219_T1 lotus 2579 51 81 100 76.2295082 blastp 1233 lotus|gb157.2|AV775053_T1 lotus 2580 51 82 84.9462366 86.8131868 blastp 1234 lotus|gb157.2|AV775249_T1 lotus 2581 51 85 95.6989247 80.9090909 blastp 1235 maize|gb164|AF326496_T1 maize 2582 51 88 100 32.4041812 blastp 1236 maize|gb164|AY107589_T1 maize 2583 51 84 100 32.8621908 blastp 1237 medicago|gb157.2| medicago 2584 51 87 100 32.4041812 blastp AI974409_T1 1238 medicago|gb157.2| medicago 2585 51 89 100 32.6315789 blastp AI974231_T1 1239 melon|gb165|EB715587_T1 melon 2586 51 86 100 23.4848485 tblastn 1240 oat|gb164|CN816056_T1 oat 2587 51 82 100 84.5454545 blastp 1241 oil_palm|gb166| oil_palm 2588 51 89 100 32.9787234 blastp CN601069_T1 1242 oil_palm|gb166| oil_palm 2589 51 84 100 32.9787234 blastp EL686181_T1 1243 peanut|gb161|CD037823_T1 peanut 2590 51 82 100 31.8493151 blastp 1244 peanut|gb161|CD038014_T1 peanut 2591 51 88 100 32.1799308 blastp 1245 periwinkle|gb164| periwinkle 2592 51 87 100 65.9574468 blastp AM232518_T2 1246 physcomitrella|gb157| physcomitrella 2593 51 89 100 33.3333333 blastp BI436955_T1 1247 physcomitrella|gb157| physcomitrella 2594 51 82 100 32.5174825 blastp BJ198543_T1 1248 physcomitrella|gb157| physcomitrella 2595 51 90 100 33.2142857 blastp AW476973_T3 1249 physcomitrella|gb157| physcomitrella 2596 51 82 100 32.5259516 blastp BJ962015_T1 1250 pine|gb157.2|AW870138_T1 pine 2597 51 82 100 34.7014925 blastp 1251 pine|gb157.2|AI813147_T1 pine 2598 51 87 100 33.0960854 blastp 1252 pine|gb157.2|AA739836_T1 pine 2599 51 87 100 33.0960854 blastp 1253 pine|gb157.2|AL750425_T1 pine 2600 51 88 100 33.0960854 blastp 1254 pine|gb157.2|BG038984_T1 pine 2601 51 80 100 32.8621908 blastp 1255 pine|gb157.2|AW225939_T1 pine 2602 51 80 100 34.1911765 blastp 1256 pine|gb157.2|BQ696500_T1 pine 2603 51 81 100 32.8621908 blastp 1257 pine|gb157.2|AL751198_T1 pine 2604 51 87 100 33.3333333 blastp 1258 pine|gb157.2|BQ695693_T1 pine 2605 51 82 100 32.8621908 blastp 1259 pine|gb157.2|BF517326_T1 pine 2606 51 80 100 60.3896104 blastp 1260 pine|gb157.2|AA739625_T1 pine 2607 51 81 100 34.7014925 blastp 1261 pine|gb157.2|BG317873_T1 pine 2608 51 82 100 32.8621908 blastp 1262 pine|gb157.2|AI813147_T2 pine 2609 51 87 100 32.9787234 blastp 1263 pine|gb157.2|CF388120_T1 pine 2610 51 87 100 33.3333333 blastp 1264 pine|gb157.2|BE662590_T1 pine 2611 51 81 100 35.7692308 blastp 1265 pine|gb157.2|BG318657_T1 pine 2612 51 82 100 32.8621908 blastp 1266 pine|gb157.2|AA557104_T1 pine 2613 51 88 100 33.3333333 blastp 1267 pine|gb157.2|AW870138_T2 pine 2614 51 82 100 32.8621908 blastp 1268 pine|gb157.2|AW289749_T1 pine 2615 51 82 100 32.8621908 blastp 1269 pine|gb157.2|H75016_T1 pine 2616 51 88 100 32.7464789 blastp 1270 pine|gb157.2|AA740005_T1 pine 2617 51 80 100 35.0943396 blastp 1271 pine|gb157.2|CA305579_T1 pine 2618 51 91 100 75.6097561 blastp 1272 pine|gb157.2|BE187350_T1 pine 2619 51 83 100 32.8621908 blastp 1273 pine|gb157.2|CF473539_T1 pine 2620 51 84 100 32.9787234 blastp 1274 pine|gb157.2|AW290370_T1 pine 2621 51 83 100 32.7464789 blastp 1275 pine|gb157.2|AW290691_T1 pine 2622 51 82 75.2688172 88.6075949 blastp 1276 pine|gb157.2|BG318695_T1 pine 2623 51 82 100 34.7014925 blastp 1277 pineapple|gb157.2| pineapple 2624 51 91 98.9247312 78.6324786 blastp DT339628_T1 1278 poplar|gb157.2|AI162483_T2 poplar 2625 51 86 100 24.3455497 tblastn 1279 poplar|gb157.2|BU817536_T4 poplar 2626 51 87 100 22.6094003 tblastn 1280 potato|gb157.2|BQ515617_T1 potato 2627 51 80 98.9247312 36.6533865 blastp 1281 potato|gb157.2|BE920139_T2 potato 2628 51 90 100 41.5178571 blastp 1282 radish|gb164|EV526963_T1 radish 2629 51 87 100 32.5174825 blastp 1283 radish|gb164|EV567230_T2 radish 2630 51 84 100 46.039604 blastp 1284 radish|gb164|EV551004_T1 radish 2631 51 92 100 32.2916667 blastp 1285 radish|gb164|EX756464_T1 radish 2632 51 86 100 48.1865285 blastp 1286 radish|gb164|EY910551_T1 radish 2633 51 91 100 31.9587629 blastp 1287 radish|gb164|EW730466_T1 radish 2634 51 84 98.9247312 87.6190476 blastp 1288 radish|gb164|AF051128_T1 radish 2635 51 81 87.0967742 48.6 tblastn 1289 radish|gb164|EX756028_T1 radish No 51 86 100 40.0286944 tblastn predicted Protein 1290 rice|gb157.2|BE229418_T1 rice 2636 51 90 100 32.9787234 blastp 1291 rice|gb157.2|BE229418_T3 rice 2637 51 90 100 38.5892116 blastp 1292 rice|gb157.2|AK107700_T1 rice 2638 51 91 100 68.8888889 blastp 1293 rice|gb157.2|BE229418_T4 rice 2639 51 90 100 54.0697674 blastp 1294 rice|gb157.2|NM001066078_T1 rice 2640 51 92 100 41.7040359 blastp 1295 rice|gb157.2|AW155505_T1 rice 2641 51 83 100 32.0689655 blastp 1296 rice|gb157.2|AW155505_T2 rice 2642 51 83 98.9247312 35.7976654 blastp 1297 rice|gb157.2|AK106746_T1 rice 2643 51 83 100 22.6461039 tblastn 1298 sorghum|gb161.xeno| sorghum 2644 51 86 100 33.3333333 blastp AY107589_T1 1299 sorghum|gb161.xeno| sorghum 2645 51 92 100 32.5174825 blastp AI947598_T1 1300 sorghum|gb161.xeno| sorghum 2646 51 80 100 31.4189189 blastp AI855413_T1 1301 sorghum|gb161.xeno| sorghum 2647 51 81 100 31.1418685 blastp AW565915_T1 1302 sorghum|gb161.xeno| sorghum 2648 51 90 100 79.6610169 blastp CF481617_T1 1303 soybean|gb166|BU549322_T1 soybean 2649 51 89 100 32.5174825 blastp 1304 soybean|gb166|BE352729_T1 soybean 2650 51 88 100 32.4041812 blastp 1305 soybean|gb166|BI974981_T1 soybean 2651 51 91 100 71.5384615 blastp 1306 soybean|gb166|BE658685_T1 soybean 2652 51 89 100 32.6315789 blastp 1307 soybean|gb166|BE352747_T3 soybean 2653 51 81 100 20.5904059 tblastn 1308 spikemoss|gb165| spikemoss 2654 51 88 100 32.0689655 blastp DN838269_T1 1309 spikemoss|gb165| spikemoss 2655 51 88 100 32.0689655 blastp FE434019_T1 1310 spruce|gb162|CO225164_T1 spruce 2656 51 89 100 32.8621908 blastp 1311 spruce|gb162|CO258147_T1 spruce 2657 51 81 100 33.8181818 blastp 1312 spruce|gb162|CO230791_T1 spruce 2658 51 88 100 46.039604 blastp 1313 spruce|gb162|DR546674_T1 spruce 2659 51 82 100 32.5 blastp 1314 spruce|gb162|DR560237_T1 spruce 2660 51 81 100 34.1911765 blastp 1315 spurge|gb161|DV116550_T1 spurge 2661 51 83 100 86.1111111 blastp 1316 sugarcane|gb157.2| sugarcane 2662 51 87 88.172043 81.1881188 blastp CA088464_T1 1317 sugarcane|gb157.2| sugarcane 2663 51 92 100 62.4161074 blastp CA086583_T1 1318 sugarcane|gb157.2| sugarcane 2664 51 86 62.3655914 100 blastp CA234165_T1 1319 sugarcane|gb157.2| sugarcane 2665 51 89 100 32.5174825 blastp CA107998_T1 1320 sugarcane|gb157.2| sugarcane 2666 51 95 100 34.1911765 tblastn CA204327_T1 1321 sugarcane|gb157.2| sugarcane 2667 51 92 86.0215054 18.6480186 tblastn CA067786_T1 1322 sunflower|gb162| sunflower 2668 51 81 100 31.9587629 blastp DY944685_T1 1323 sunflower|gb162| sunflower 2669 51 87 52.688172 100 blastp BQ968590_T1 1324 sunflower|gb162| sunflower 2670 51 89 98.9247312 41.3173653 tblastn CD848850_T1 1325 tobacco|gb162|EB445876_T1 tobacco 2671 51 90 100 32.4041812 blastp 1326 tobacco|gb162|EB445188_T1 tobacco 2672 51 88 100 32.4041812 blastp 1327 tobacco|gb162|CK720586_T1 tobacco 2673 51 81 100 34.7014925 blastp 1328 tomato|gb164|CO750818_T1 tomato 2674 51 92 88.172043 82.8282828 blastp 1329 tomato|gb164|AI772191_T1 tomato 2675 51 81 98.9247312 33.6996337 blastp 1330 tomato|gb164|AW219533_T2 tomato 2676 51 89 100 39.2405063 tblastn 1331 triphysaria|gb164| triphysaria 2677 51 88 95.6989247 80.9090909 blastp DR173305_T1 1332 triphysaria|gb164| triphysaria 2678 51 90 100 75.6097561 blastp EX984185_T1 1333 triphysaria|gb164| triphysaria 2679 51 91 100 67.8832117 blastp DR174019_T1 1334 wheat|gb164|CA609068_T1 wheat 2680 51 94 100 67.3913043 blastp 1335 wheat|gb164|BE430411_T1 wheat 2681 51 86 100 32.4041812 blastp 1336 wheat|gb164|CK208980_T1 wheat 2682 51 80 100 30 blastp 1337 wheat|gb164|CA620158_T1 wheat 2683 51 96 95.6989247 87.254902 blastp 1338 wheat|gb164|BE405395_T1 wheat 2684 51 88 100 32.7464789 blastp 1339 wheat|gb164|CA647310_T1 wheat 2685 51 80 77.4193548 93.5064935 blastp 1340 wheat|gb164|BE492099_T1 wheat 2686 51 87 100 32.7464789 blastp 1341 wheat|gb164|BE402029_T1 wheat 2687 51 87 100 32.7464789 blastp 1342 wheat|gb164|CA618130_T1 wheat 2688 51 86 88.172043 73.2142857 blastp 1343 wheat|gb164|CJ605707_T1 wheat 2689 51 82 100 69.4029851 blastp 1344 wheat|gb164|CA614209_T1 wheat 2690 51 98 59.1397849 98.2142857 blastp 1345 wheat|gb164|CA701714_T1 wheat 2691 51 82 100 77.5 blastp 1346 wheat|gb164|BE403921_T1 wheat 2692 51 93 96.7741935 82.5688073 blastp 1347 wheat|gb164|BE217049_T1 wheat 2693 51 83 100 31.9587629 blastp 1348 wheat|gb164|CA602649_T1 wheat 2694 51 84 100 30.0322928 tblastn 1349 wheat|gb164|CA486220_T1 wheat 2695 51 82 96.7741935 33.3759591 tblastn 1350 b_juncea|gb164| b_juncea 2696 52 85 64.3835616 99.2957746 blastp EVGN00044413933329_T1 1351 b_juncea|gb164| b_juncea 2697 52 90 53.4246575 99.1525424 blastp EVGN00137910990746_T1 1352 barley|gb157.3|BE413268_T1 barley 2698 52 81 96.803653 73.4482759 blastp 1353 barley|gb157.3|AJ433979_T1 barley 2699 52 81 96.803653 73.4482759 blastp 1354 barley|gb157.3|BE412979_T1 barley 2700 52 82 96.803653 74.1258741 blastp 1355 brachypodium|gb161.xeno| brachypodium 2701 52 83 96.803653 73.6111111 blastp AF139815_T1 1356 citrus|gb157.2|CX052950_T1 citrus 2702 52 84 50.2283105 100 blastp 1357 clover|gb162|BB913131_T1 clover 2703 52 93 52.5114155 99.137931 blastp 1358 clover|gb162|BB918704_T1 clover 2704 52 82 63.9269406 99.2957746 blastp 1359 cotton|gb164|CO103246_T1 cotton 2705 52 91 56.6210046 93.9393939 blastp 1360 cowpea|gb166|FG883860_T1 cowpea 2706 52 83 51.1415525 99.1150442 blastp 1361 fescue|gb161|DT702489_T1 fescue 2707 52 96 57.0776256 93.2835821 blastp 1362 fescue|gb161|DT702846_T1 fescue 2708 52 96 57.0776256 96.8992248 blastp 1363 ipomoea|gb157.2| ipomoea 2709 52 81 87.2146119 74.7035573 blastp BU691146_T1 1364 maize|gb164|AI939909_T1 maize 2710 52 80 96.803653 73.6111111 blastp 1365 maize|gb164|AF130975_T1 maize 2711 52 83 96.803653 74.3859649 blastp 1366 maize|gb164|AI947831_T1 maize 2712 52 83 96.803653 73.6111111 blastp 1367 oil_palm|gb166| oil_palm 2713 52 80 96.803653 75.177305 blastp EL692065_T1 1368 physcomitrella|gb157| physcomitrella 2714 52 80 93.6073059 73.4767025 blastp AW476973_T1 1369 physcomitrella|gb157| physcomitrella 2715 52 80 96.347032 73.0103806 blastp BI894596_T1 1370 physcomitrella|gb157| physcomitrella 2716 52 80 93.6073059 73.4767025 blastp AW476973_T2 1371 pine|gb157.2|CF387570_T1 pine 2717 52 88 51.1415525 99.1150442 blastp 1372 radish|gb164|EY904434_T2 radish 2718 52 88 54.3378995 99.1666667 blastp 1373 radish|gb164|FD960377_T1 radish 2719 52 84 63.0136986 99.2805755 blastp 1374 rice|gb157.2|AU093957_T1 rice 2720 52 81 96.803653 74.1258741 blastp 1375 rice|gb157.2|BE039992_T2 rice 2721 52 92 57.0776256 97.65625 blastp 1376 rice|gb157.2|BE530955_T1 rice 2722 52 80 96.803653 73.1034483 blastp 1377 rye|gb164|BE586469_T1 rye 2723 52 82 61.1872146 97.810219 blastp 1378 sorghum|gb161.xeno| sorghum 2724 52 83 96.803653 72.6027397 blastp AW922622_T1 1379 sorghum|gb161.xeno| sorghum 2725 52 80 85.3881279 74.8 blastp BE344582_T1 1380 sorghum|gb161.xeno| sorghum 2726 52 82 96.803653 73.3564014 blastp AI855280_T1 1381 spikemoss|gb165| spikemoss 2727 52 83 91.7808219 71.5302491 blastp DN838148_T1 1382 spikemoss|gb165| spikemoss 2728 52 83 91.7808219 71.5302491 blastp DN838057_T1 1383 sugarcane|gb157.2| sugarcane 2729 52 82 90.4109589 71.8978102 blastp CA139573_T1 1384 sugarcane|gb157.2| sugarcane 2730 52 90 57.0776256 99.2063492 blastp CA145403_T1 1385 sunflower|gb162| sunflower 2731 52 80 57.0776256 93.2835821 blastp CF083179_T1 1386 sunflower|gb162| sunflower 2732 52 86 56.6210046 89.2086331 blastp BU035823_T1 1387 switchgrass|gb165| switchgrass 2733 52 83 61.6438356 99.2647059 blastp DN140790_T1 1388 switchgrass|gb165| switchgrass 2734 52 81 66.2100457 96.6666667 blastp FE631354_T1 1389 tobacco|gb162|DV159802_T1 tobacco 2735 52 81 87.6712329 48.7722269 tblastn 1390 wheat|gb164|AF139815_T1 wheat 2736 52 82 96.803653 74.1258741 blastp 1391 wheat|gb164|BE406301_T1 wheat 2737 52 81 96.803653 73.4482759 blastp 1392 wheat|gb164|BE404904_T1 wheat 2738 52 81 96.803653 73.4482759 blastp 1393 wheat|gb164|BE400219_T1 wheat 2739 52 81 96.803653 73.4482759 blastp 1394 wheat|gb164|CA619093_T1 wheat 2740 52 81 54.7945205 90.1515152 blastp 1395 wheat|gb164|BE605056_T1 wheat 2741 52 88 56.6210046 93.2330827 blastp 1396 wheat|gb164|BQ245211_T1 wheat 2742 52 82 96.803653 74.1258741 blastp 1397 wheat|gb164|BE497487_T1 wheat 2743 52 81 96.803653 73.4482759 blastp 1398 wheat|gb164|BQ295206_T1 wheat 2744 52 89 64.8401826 100 blastp 1399 wheat|gb164|BE499954_T1 wheat 2745 52 80 98.630137 74.8275862 blastp 1400 wheat|gb164|BE405794_T1 wheat 2746 52 81 96.803653 73.4482759 blastp 5 tomato|gb164|BP881534_T1 tomato 31 32 82 100 100 blastp 21 barley|gb157.3|AL501410_T1 barley 47 46 87 98.3935743 98.79518072 blastp 2844 antirrhinum|gb166| antirrhinum 3052 25 88 100 100 blastp AJ559427_T1 2845 antirrhinum|gb166| antirrhinum 3053 25 85 100 100 blastp AJ791214_T1 2846 bruguiera|gb166| bruguiera 3054 25 82 61.29032258 100 blastp BP939664_T1 2847 centaurea|gb166| centaurea 3055 25 84 98.79032258 100 blastp EL931601_T1 2848 eucalyptus|gb166| eucalyptus 3056 25 85 98.79032258 98.79032258 blastp CD668425_T1 2849 kiwi|gb166|FG409998_T1 kiwi 3057 25 85 100 100 blastp 2850 kiwi|gb166|FG453166_T1 kiwi 3058 25 86 100 100 blastp 2851 kiwi|gb166|FG401585_T1 kiwi 3059 25 87 99.59677419 100 blastp 2852 kiwi|gb166|FG419790_T1 kiwi 3060 25 85 100 100 blastp 2853 liriodendron|gb166| liriodendron 3061 25 82 99.19354839 98.79518072 blastp FD495170_T1 2854 poppy|gb166|FG607362_T1 poppy 3062 25 82 82.66129032 99.51456311 blastp 2855 soybean|gb167|AA660186_T1 soybean 3063 25 83 100 100 blastp 2856 soybean|gb167|CA990807_T1 soybean 3064 25 82 95.56451613 100 blastp 2857 walnuts|gb166|CV196664_T1 walnuts 3065 25 83 100 100 blastp 2858 antirrhinum|gb166| antirrhinum 3066 26 90 100 100 blastp X70417_T1 2859 banana|gb167|FF560721_T1 banana 3067 26 86 77.6 99.48717949 blastp 2860 centaurea|gb166| centaurea 3068 26 86 100 100 blastp EL932548_T1 2861 antirrhinum|gb166| antirrhinum 3069 27 86 90.90909091 100 blastp AJ789802_T1 2862 bruguiera|gb166| bruguiera 3070 27 81 69.96047431 100 blastp BP938735_T1 2863 eucalyptus|gb166| eucalyptus 3071 27 82 90.90909091 100 blastp ES589574_T1 2864 kiwi|gb166|FG427735_T1 kiwi 3072 27 86 50.19762846 99.21875 blastp 2865 kiwi|gb166|FG406415_T1 kiwi 3073 27 81 54.15019763 100 blastp 2866 kiwi|gb166|FG406885_T1 kiwi 3074 27 84 99.20948617 99.6031746 blastp 2867 liriodendron|gb166| liriodendron 3075 27 81 99.20948617 99.6031746 blastp CK744430_T1 2868 poppy|gb166|FG608493_T1 poppy 3076 27 84 83.00395257 99.52606635 blastp 2869 soybean|gb167|EV269611_T1 soybean 3077 27 86 60.07905138 96.20253165 blastp 2870 amborella|gb166| amborella 3078 28 82 100 100 blastp CD482678_T1 2871 cenchrus|gb166| cenchrus 3079 28 81 100 100 blastp BM084541_T1 2872 leymus|gb166|EG376267_T1 leymus 3080 28 80 100 100 blastp 2873 leymus|gb166|EG386149_T1 leymus 3081 28 80 100 100 blastp 2874 walnuts|gb166|EL895384_T1 walnuts 3082 28 83 82 94.49541284 blastp 2875 amborella|gb166| amborella 3083 30 88 98.26388889 98.95470383 blastp CD481950_T1 2876 antirrhinum|gb166| antirrhinum 3084 30 86 93.40277778 100 blastp AJ796874_T1 2877 antirrhinum|gb166| antirrhinum 3085 30 84 73.26388889 99.52606635 blastp AJ798039_T1 2878 antirrhinum|gb166| antirrhinum 3086 30 88 85.06944444 100 blastp AJ792331_T1 2879 antirrhinum|gb166| antirrhinum 3087 30 86 98.26388889 99.3006993 blastp AJ558770_T1 2880 banana|gb167|FF558844_T1 banana 3088 30 88 98.26388889 98.95104895 blastp 2881 bean|gb167|CA898412_T1 bean 3089 30 81 98.26388889 99.30313589 blastp 2882 bruguiera|gb166| bruguiera 3090 30 86 52.77777778 95 blastp BP941115_T1 2883 bruguiera|gb166| bruguiera 3091 30 85 97.91666667 99.30313589 blastp BP939033_T1 2884 cenchrus|gb166| cenchrus 3092 30 85 98.26388889 98.95833333 blastp EB656428_T1 2885 centaurea|gb166| centaurea 3093 30 86 98.26388889 98.95470383 blastp EH767475_T1 2886 centaurea|gb166| centaurea 3094 30 86 98.26388889 98.95833333 blastp EL935569_T1 2887 cycas|gb166|CB089724_T1 cycas 3095 30 84 98.26388889 98.95470383 blastp 2888 cycas|gb166|CB088798_T1 cycas 3096 30 83 98.26388889 98.26989619 blastp 2889 eucalyptus|gb166| eucalyptus 3097 30 84 98.26388889 99.30555556 blastp CD668044_T1 2890 eucalyptus|gb166| eucalyptus 3098 30 87 98.26388889 98.95470383 blastp AW191311_T1 2891 kiwi|gb166|FG403284_T1 kiwi 3099 30 85 98.26388889 99.3006993 blastp 2892 kiwi|gb166|FG404130_T1 kiwi 3100 30 86 98.26388889 99.3006993 blastp 2893 kiwi|gb166|FG396354_T1 kiwi 3101 30 90 98.26388889 98.95104895 blastp 2894 kiwi|gb166|FG404890_T1 kiwi 3102 30 85 72.22222222 99.5215311 blastp 2895 kiwi|gb166|FG417962_T1 kiwi 3103 30 87 62.15277778 99.44444444 blastp 2896 kiwi|gb166|FG403188_T1 kiwi 3104 30 89 98.26388889 96.91780822 blastp 2897 kiwi|gb166|FG403647_T1 kiwi 3105 30 85 68.05555556 99.49238579 blastp 2898 kiwi|gb166|FG397405_T1 kiwi 3106 30 85 98.26388889 99.3006993 blastp 2899 leymus|gb166|EG384635_T1 leymus 3107 30 85 99.30555556 99.65517241 blastp 2900 leymus|gb166|CN466016_T1 leymus 3108 30 83 98.26388889 98.97260274 blastp 2901 leymus|gb166|CN466006_T1 leymus 3109 30 83 98.26388889 98.97260274 blastp 2902 leymus|gb166|EG376500_T1 leymus 3110 30 83 98.26388889 98.97260274 blastp 2903 liriodendron|gb166| liriodendron 3111 30 89 98.26388889 98.95470383 blastp CK749885_T1 2904 liriodendron|gb166| liriodendron 3112 30 88 98.26388889 98.95470383 blastp CV002697_T1 2905 lovegrass|gb167| lovegrass 3113 30 85 98.26388889 98.96193772 blastp DN480914_T1 2906 nuphar|gb166|CD472824_T1 nuphar 3114 30 86 98.26388889 98.94736842 blastp 2907 nuphar|gb166|CD472574_T1 nuphar 3115 30 86 98.26388889 98.94736842 blastp 2908 nuphar|gb166|CD473614_T1 nuphar 3116 30 84 51.73611111 98.02631579 blastp 2909 peanut|gb167|ES759056_T1 peanut 3117 30 83 64.23611111 100 blastp 2910 poppy|gb166|FE966578_T1 poppy 3118 30 83 98.61111111 98.62068966 blastp 2911 pseudoroegneria| pseudoroegneria 3119 30 82 98.26388889 98.97260274 blastp gb167|FF344096_T1 2912 pseudoroegneria| pseudoroegneria 3120 30 85 98.61111111 98.62542955 blastp gb167|FF346975_T1 2913 pseudoroegneria| pseudoroegneria 3121 30 83 98.26388889 98.97260274 blastp gb167|FF342094_T1 2914 soybean|gb167|CA898412_T1 soybean 3122 30 84 94.44444444 95.0877193 blastp 2915 switchgrass|gb167| switchgrass 3123 30 87 52.43055556 98.69281046 blastp FE621985_T1 2916 switchgrass|gb167| switchgrass 3124 30 86 98.26388889 98.95833333 blastp DN141371_T1 2917 tamarix|gb166|CF199714_T1 tamarix 3125 30 88 78.47222222 99.12280702 blastp 2918 tamarix|gb166|CF226851_T1 tamarix 3126 30 85 98.26388889 99.30313589 blastp 2919 walnuts|gb166|CB303847_T1 walnuts 3127 30 84 98.26388889 99.31034483 blastp 2920 walnuts|gb166|CV194951_T1 walnuts 3128 30 86 98.26388889 99.30313589 blastp 2921 walnuts|gb166|CV196162_T1 walnuts 3129 30 85 98.26388889 99.31506849 blastp 2922 zamia|gb166|FD765004_T1 zamia 3130 30 84 98.26388889 98.95470383 blastp 2923 antirrhinum|gb166| antirrhinum 3131 31 80 98.7854251 100 blastp AJ799752_T1 2924 kiwi|gb166|FG400670_T1 kiwi 3132 31 82 74.08906883 100 blastp 2925 kiwi|gb166|FG418275_T1 kiwi 3133 31 83 98.7854251 98.38709677 blastp 2926 centaurea|gb166| centaurea 3134 34 83 88.57142857 32.74021352 blastp EL931588_T1 2927 soybean|gb167|AW119586_T1 soybean 3135 34 83 94.28571429 36.26373626 blastp 2928 soybean|gb167|AW573764_T1 soybean 3136 34 83 94.28571429 36.66666667 blastp 2929 amborella|gb166| amborella 3137 35 81 65.76271186 100 blastp FD440187_T1 2930 centaurea|gb166| centaurea 3138 35 82 97.62711864 96.61016949 blastp EL931433_T1 2931 eucalyptus|gb166| eucalyptus 3139 35 85 73.89830508 100 blastp CD668486_T1 2932 petunia|gb166|DC243166_T1 petunia 3140 36 86 100 100 blastp 2933 amborella|gb166| amborella 3141 39 82 99.64157706 98.93992933 blastp CD482946_T1 2934 antirrhinum|gb166| antirrhinum 3142 39 88 99.28315412 98.57651246 blastp AJ559435_T1 2935 antirrhinum|gb166| antirrhinum 3143 39 89 71.32616487 100 blastp AJ793990_T1 2936 antirrhinum|gb166| antirrhinum 3144 39 83 61.29032258 87.24489796 blastp AJ559760_T1 2937 antirrhinum|gb166| antirrhinum 3145 39 88 88.53046595 100 blastp AJ558545_T1 2938 bean|gb167|FE682762_T1 bean 3146 39 86 100 100 blastp 2939 bean|gb167|CV531088_T1 bean 3147 39 86 99.64157706 98.94736842 blastp 2940 bean|gb167|CA907460_T1 bean 3148 39 86 99.64157706 98.94736842 blastp 2941 cenchrus|gb166| cenchrus 3149 39 83 97.49103943 95.86206897 blastp EB655519_T1 2942 centaurea|gb166| centaurea 3150 39 88 53.76344086 96.15384615 blastp EL934360_T1 2943 centaurea|gb166| centaurea 3151 39 80 88.53046595 100 blastp EH751120_T1 2944 centaurea|gb166| centaurea 3152 39 87 83.5125448 100 blastp EH752971_T1 2945 centaurea|gb166| centaurea 3153 39 80 91.7562724 99.61685824 blastp EH768434_T1 2946 centaurea|gb166| centaurea 3154 39 83 83.15412186 100 blastp EH767287_T1 2947 cichorium|gb166| cichorium 3155 39 82 89.96415771 100 blastp DT212008_T1 2948 cichorium|gb166| cichorium 3156 39 84 86.73835125 97.61904762 blastp EL354583_T1 2949 eucalyptus|gb166| eucalyptus 3157 39 87 83.87096774 100 blastp CD668553_T1 2950 eucalyptus|gb166| eucalyptus 3158 39 80 69.89247312 100 blastp CD668534_T1 2951 eucalyptus|gb166| eucalyptus 3159 39 88 100 99.3006993 blastp CD668523_T1 2952 eucalyptus|gb166| eucalyptus 3160 39 83 100 100 blastp CD669942_T1 2953 kiwi|gb166|FG405216_T1 kiwi 3161 39 84 100 99.29328622 blastp 2954 kiwi|gb166|FG495821_T1 kiwi 3162 39 86 50.17921147 100 blastp 2955 kiwi|gb166|FG417997_T1 kiwi 3163 39 86 64.51612903 100 blastp 2956 kiwi|gb166|FG403725_T1 kiwi 3164 39 83 73.11827957 100 blastp 2957 kiwi|gb166|FG397310_T1 kiwi 3165 39 88 100 100 blastp 2958 kiwi|gb166|FG408531_T1 kiwi 3166 39 83 100 99.30313589 blastp 2959 leymus|gb166|EG381236_T1 leymus 3167 39 81 55.55555556 97.46835443 blastp 2960 leymus|gb166|EG376087_T1 leymus 3168 39 80 96.77419355 96.55172414 blastp 2961 leymus|gb166|CD808804_T1 leymus 3169 39 81 100 100 blastp 2962 leymus|gb166|EG378918_T1 leymus 3170 39 86 51.25448029 100 blastp 2963 liriodendron|gb166| liriodendron 3171 39 81 99.64157706 98.96193772 blastp CK761396_T1 2964 nuphar|gb166|ES730700_T1 nuphar 3172 39 81 61.29032258 98.27586207 blastp 2965 poppy|gb166|FE965621_T1 poppy 3173 39 84 88.53046595 100 blastp 2966 pseudoroegneria| pseudoroegneria 3174 39 81 100 100 blastp gb167|FF340233_T1 2967 switchgrass|gb167| switchgrass 3175 39 80 100 100 blastp FE638368_T1 2968 switchgrass|gb167| switchgrass 3176 39 80 99.28315412 98.95833333 blastp FE657460_T1 2969 switchgrass|gb167| switchgrass 3177 39 82 78.85304659 86.59003831 blastp FE641178_T1 2970 switchgrass|gb167| switchgrass 3178 39 82 94.26523297 95.72953737 blastp FE619224_T1 2971 switchgrass|gb167| switchgrass 3179 39 81 100 100 blastp FE657460_T2 2972 tamarix|gb166|EH051524_T1 tamarix 3180 39 82 56.27240143 98.74213836 blastp 2973 walnuts|gb166|CB304207_T1 walnuts 3181 39 84 100 99.30313589 blastp 2974 walnuts|gb166|CB303561_T1 walnuts 3182 39 84 100 100 blastp 2975 walnuts|gb166|EL892579_T1 walnuts 3183 39 84 89.60573477 98.82352941 blastp 2976 zamia|gb166|DY032141_T1 zamia 3184 39 80 96.05734767 94.32624113 blastp 2977 zamia|gb166|FD764669_T1 zamia 3185 39 80 99.64157706 98.92473118 blastp 2978 zamia|gb166|DY034152_T1 zamia 3186 39 80 94.62365591 92.25352113 blastp 2979 antirrhinum|gb166| antirrhinum 3187 40 87 100 100 blastp AJ568195_T1 2980 antirrhinum|gb166| antirrhinum 3188 40 87 100 100 blastp AJ568110_T1 2981 banana|gb167|FL657842_T1 banana 3189 40 81 83.03886926 92.49011858 blastp 2982 bruguiera|gb166| bruguiera 3190 40 84 100 100 blastp EF126757_T1 2983 centaurea|gb166| centaurea 3191 40 84 92.5795053 100 blastp EH765776_T1 2984 eucalyptus|gb166| eucalyptus 3192 40 85 100 100 blastp AJ627837_T1 2985 kiwi|gb166|FG411924_T1 kiwi 3193 40 86 100 100 blastp 2986 kiwi|gb166|FG400706_T1 kiwi 3194 40 85 100 100 blastp 2987 kiwi|gb166|FG418898_T1 kiwi 3195 40 84 71.02473498 98.5 blastp 2988 kiwi|gb166|FG420187_T1 kiwi 3196 40 85 72.79151943 100 blastp 2989 kiwi|gb166|FG398010_T1 kiwi 3197 40 86 100 100 blastp 2990 petunia|gb166|AF452012_T1 petunia 3198 40 88 100 100 blastp 2991 tamarix|gb166|CV121772_T1 tamarix 3199 40 83 100 100 blastp 2992 walnuts|gb166|EL893208_T1 walnuts 3200 40 84 100 100 blastp 2993 bean|gb167|FD786218_T1 bean 3201 41 83 58.60927152 100 blastp 2994 citrus|gb166|CX074333_T2 citrus 3202 41 81 72.51655629 99.5412844 blastp 2995 citrus|gb166|CX074333_T1 citrus 3203 41 83 96.02649007 91.42857143 blastp 2996 kiwi|gb166|FG420468_T2 kiwi 3204 41 84 87.08609272 97.04797048 blastp 2997 kiwi|gb166|FG420468_T1 kiwi 3205 41 83 58.94039735 95.69892473 blastp 2998 tamarix|gb166|EG972096_T1 tamarix 3206 42 82 60.97560976 95.23809524 blastp 2999 pseudoroegneria| pseudoroegneria 3207 43 86 98.3935743 98.79518072 blastp gb167|FF353501_T1 3000 switchgrass|gb167| switchgrass 3208 43 86 98.79518072 99.19678715 blastp FE646459_T1 3001 switchgrass|gb167| switchgrass 3209 43 84 71.48594378 93.22916667 blastp FL887003_T1 3002 wheat|gb164|BE403397_T1 wheat 3210 43 86 98.3935743 98.79518072 blastp 3003 leymus|gb166|EG381168_T1 leymus 3211 44 96 52.01612903 100 blastp 3004 pseudoroegneria| pseudoroegneria 3212 44 97 100 100 blastp gb167|FF341567_T1 3005 switchgrass|gb167| switchgrass 3213 44 91 100 100 blastp FE618822_T1 3006 switchgrass|gb167| switchgrass 3214 44 92 78.22580645 100 blastp FE633786_T1 3007 eucalyptus|gb166| eucalyptus 3215 46 84 97.51552795 60.78431373 blastp CB967586_T1 3008 liriodendron|gb166| liriodendron 3216 46 80 74.53416149 100 blastp CK762443_T1 3009 switchgrass|gb167| switchgrass 3217 46 90 100 61.38996139 blastp FE615387_T1 3010 walnuts|gb166|EL893973_T1 walnuts 3218 46 82 97.51552795 55.47445255 blastp 3011 bean|gb167|FD782805_T1 bean 3219 47 91 88.17204301 79.61165049 blastp 3012 bean|gb167|EY457935_T1 bean 3220 47 82 100 38.58921162 blastp 3013 cichorium|gb166| cichorium 3221 47 80 100 67.39130435 blastp EH685648_T2 3014 cichorium|gb166| cichorium 3222 47 80 100 32.1799308 blastp EH685648_T1 3015 citrus|gb166|CK701147_T1 citrus 3223 47 90 100 76.85950413 blastp 3016 citrus|gb166|CX544905_T1 citrus 3224 47 81 79.56989247 88.0952381 blastp 3017 cycas|gb166|CB088978_T1 cycas 3225 47 82 87.09677419 30.68181818 blastp 3018 cycas|gb166|EX920749_T1 cycas 3226 47 88 100 76.2295082 blastp 3019 kiwi|gb166|FG429765_T1 kiwi 3227 47 84 98.92473118 77.96610169 blastp 3020 leymus|gb166|CN466394_T1 leymus 3228 47 87 100 79.48717949 blastp 3021 leymus|gb166|EG376019_T1 leymus 3229 47 88 100 32.74647887 blastp 3022 leymus|gb166|EG390723_T1 leymus 3230 47 81 100 31.95876289 blastp 3023 leymus|gb166|EG387193_T1 leymus 3231 47 81 100 32.1799308 blastp 3024 nuphar|gb166|CD473277_T1 nuphar 3232 47 91 100 75.6097561 blastp 3025 nuphar|gb166|FD384794_T1 nuphar 3233 47 86 96.77419355 81.08108108 blastp 3026 nuphar|gb166|CD475538_T1 nuphar 3234 47 89 100 69.92481203 blastp 3027 nuphar|gb166|CD472711_T1 nuphar 3235 47 91 100 48.94736842 blastp 3028 petunia|gb166|DC240378_T1 petunia 3236 47 92 58.06451613 98.18181818 blastp 3029 pseudoroegneria| pseudoroegneria 3237 47 87 100 32.74647887 blastp gb167|FF344283_T1 3030 sorghum|gb161.crp| sorghum 3238 47 92 100 32.51748252 blastp AI724931_T1 3031 switchgrass|gb167| switchgrass 3239 47 82 100 33.69565217 blastp FL923354_T1 3032 switchgrass|gb167| switchgrass 3240 47 92 98.92473118 74.79674797 blastp FE657461_T1 3033 switchgrass|gb167| switchgrass 3241 47 82 100 72.22222222 blastp FL765830_T1 3034 switchgrass|gb167| switchgrass 3242 47 83 97.84946237 87.5 blastp FL915169_T1 3035 tamarix|gb166|EH054604_T1 tamarix 3243 47 83 100 65.64705882 tblastn 3036 walnuts|gb166|CB303798_T1 walnuts 3244 47 91 100 80.17241379 blastp 3037 bruguiera|gb166| bruguiera 3245 48 82 52.05479452 100 blastp BP942548_T1 3038 bruguiera|gb166| bruguiera 3246 48 89 56.62100457 91.17647059 blastp BP938825_T1 3039 centaurea|gb166| centaurea 3247 48 83 87.21461187 50 tblastn EH739326_T1 3040 eucalyptus|gb166| eucalyptus 3248 48 87 56.16438356 91.79104478 blastp CD669176_T1 3041 leymus|gb166|EG382428_T1 leymus 3249 48 80 52.05479452 90.47619048 blastp 3042 liriodendron|gb166| liriodendron 3250 48 81 94.06392694 54.54545455 tblastn CK743477_T1 3043 marchantia|gb161| marchantia 3251 48 83 94.06392694 72.53521127 blastp BJ840587_T1 3044 marchantia|gb166| marchantia 3252 48 83 93.15068493 71.57894737 blastp C96070_T1 3045 nuphar|gb166|CK748374_T1 nuphar 3253 48 82 65.75342466 99.31034483 blastp 3046 pseudoroegneria| pseudoroegneria 3254 48 81 96.80365297 73.44827586 blastp gb167|FF340047_T1 3047 pseudoroegneria| pseudoroegneria 3255 48 81 96.80365297 73.44827586 blastp gb167|FF340899_T1 3048 pseudoroegneria| pseudoroegneria 3256 48 82 96.80365297 74.12587413 blastp gb167|FF352644_T1 3049 sorghum|gb161.crp| sorghum 3257 48 80 96.80365297 74.12587413 blastp SBGWP030188_T1 3050 switchgrass|gb167| switchgrass 3258 48 81 96.80365297 74.12587413 blastp FL718379_T1 3051 switchgrass|gb167| switchgrass 3259 48 82 96.80365297 73.10344828 blastp FE639195_T1 Table 3: Homologues and orthologues of the AQP proteins are provided. Homology was calculated as % of identity over the aligned sequences. Polynuc. = Polynucleotide; Polypep. = Polypeptide; Hom. = Homologues/Orthologues; % Ident. = percent identity; Cover. = coverage.

Example 2 mRNA Expression of In-Silico Expressed Polynucleotides

Messenger RNA levels were determined using reverse transcription assay followed by quantitative Real-Time PCR (qRT-PCR) analysis. RNA levels were compared between leaves of 20 days old seedlings of tomato plants grown under salinity water. A correlation analysis between mRNA levels in different experimental conditions/genetic backgrounds was performed in order to determine the role of the gene in the plant.

Materials and Experimental Methods

Quantitative Real Time RT-PCR (qRT-PCR)—

To verify the level of expression, specificity and trait-association, Reverse Transcription followed by quantitative Real-Time PCR (qRTPCR) was performed on total RNA extracted from leaves of 2 tomato varieties namely YO0361 (salt tolerant variety) and FA191 (salt sensitive variety). Messenger RNA (mRNA) levels were determined for AQP genes, expressed under normal and stressed conditions.

Twenty days-old tomato seedlings were grown in soil and soaked with 300 mM NaCl for 0, 1, 6, 24, 118 hours. Leaves were harvested and frozen in liquid nitrogen and then kept at −80° C. until RNA extraction. Total RNA was extracted from leaves using RNeasy plant mini kit (Qiagen, Hilden, Germany) and by using the protocol provided by the manufacturer. Reverse transcription was performed using 1 μg total RNA, using 200 U Super Script II Reverse Transcriptase enzyme (Invitrogen), 150 ng random deoxynucleotide hexamers (Invitrogen), 500 μM deoxynucleotide tri-phosphates (dNTPs) mix (Takara, Japan), 0.2 volume of x5 reverse transcriptase (RT) buffer (Invitrogen), 0.01 M dithiothreitol (DTT), 40 U RNAsin (Promega), diethylpyrocarbonate (DEPC) treated double distilled water (DDW) was added up to 24 μl.

Mix of RNA, random deoxynucleotide hexamers, dNTPs mix and DEPC treated DDW was incubated at 65° C. for 5 minutes, followed by 4° C. for 5 minutes. Mix of reverse transcriptase (RT) buffer, dithiothreitol (DTT) and RNAsin was added to the RT reactions followed by incubation at 25° C. for 10 minutes and at 42° C. for 2 minutes afterwards. Finally, Super Script II Reverse Transcriptase enzyme was added to the RT reactions that were further incubated for 50 minutes at 42° C., followed by 70° C. for 15 minutes.

cDNA was diluted 1:20 in Tris EDTA, pH=7.5 for MAB69 and housekeeping genes. For MAB58 and MAB59 cDNA was diluted 1:2 due to very weak expression and consequently for housekeeping genes cDNA was diluted 1:8 in order to insert the Ct values in calibration curve range. 5 μL of the diluted cDNA was used for qPCR.

For qPCR amplification, primers of the AQP genes were designed, as summarized in Table 4 below. The expression level of the housekeeping genes: Actin (SEQ ID NO: 2841), GAPDH (SEQ ID NO: 2842) and RPL19 (SEQ ID NO: 2747) was determined in order to normalize the expression level between the different tissues.

TABLE 4 Primers for qPCR amplification Forward Reverse primer primer SEQ ID Forward primer SEQ ID Reverse primer Gene NO: sequence (5′→3′) NO: sequence (5′→3′) MAB58 2829 CTTTTGGTAGGGCCGATGA 2830 CGAAGATGAAGGTGGA AG TAAGAGCT MAB59 2831 CAGTATGAACGTCTCCGGT 2832 CAACAGCACCTAGCAA GG CTGACC MAB69 2833 TGTCTTGGATTCCATTGAGC 2834 GTTTGAGCTGCTGTCCC ACT CA Actin 2835 CCACATGCCATTCTCCGTCT 2836 GCTTTTCTTTCACGTCC (SEQ CTGA ID NO: 2841) GAPD 2837 TTGTTGTGGGTGTCAACGAG 2838 ATGGCGTGGACAGTGG H (SEQ A TCA ID NO: 2842) RPL19 2839 CACTCTGGATATGGTAAGC 2840 TTCTTGGACTCCCTGTA (SEQ GTAAGG CTTACGA ID NO: 2747)

Experimental Results

Changes in mRNA Levels of AQP Genes in Leaves of Plants Under Salt Tolerance—

Steady state levels of tomato AQP genes in leaves of tolerant versus sensitive lines, under salinity conditions are summarized in Table 5 below. In all 3 cases, aquaporin gene expression was increased after plant was exposed to salt stress. Gene peak expression was higher in the salt tolerance tomato line (YO361) versus the sensitive line (FA191). The elevated gene expression demonstrates the involvement of the tested AQP genes in tomato plants tolerating high salinity.

TABLE 5 Expression levels of tomato AQP genes Well name (cDNA) MAB58 MAB59 MAB69* leaf FA191 0 h 2.86 5.97 875 leaf FA191 1 h 4.56 5.73 597 leaf FA191 6 h 5.34 8.2 945 leaf FA191 24 h 42.7 62.4 613 leaf FA191 118 h 55.4 22.2 1800 leaf Y0361 0 h 1.96 2.81 638 leaf Y0361 1 h 2.33 0.517 583 leaf Y0361 6 h 2.88 5.66 464 leaf Y0361 24 h 75.4 139 513 leaf Y0361 118 h 26.3 Not determined 2300 Table 5: Provided are the steady state levels of tomato AQP genes under salinity conditions [the incubation periods in the salt solution are provided in hours (h)]. Different dilutions of cDNA were used (1:20 for MAB69 and 1:2 for MAB58 and MAB59). Numbers are given after normalization for each sample.

Example 3 Gene Cloning and Generation of Binary Vectors for Plant Expression

To validate their role in improving ABST and yield, the AQP genes were over-expressed in plants, as follows.

Cloning Strategy

Selected genes from those presented in Example 1 were cloned into binary vectors for the generation of transgenic plants. For cloning, the full-length open reading frames (ORFs) were identified. EST clusters and in some cases mRNA sequences were analyzed to identify the entire open reading frame by comparing the results of several translation algorithms to known proteins from other plant species. In case where the entire coding sequence is not found, RACE kits from Ambion or Clontech (RACE=Rapid Access to cDNA Ends) were used to prepare RNA from the plant samples to thereby access the full cDNA transcript of the gene.

In order to clone the full-length cDNAs, Reverse Transcription followed by PCR (RT-PCR) was performed on total RNA extracted from leaves, roots or other plant tissues, growing under either normal or nutrient deficient conditions. Total RNA extraction, production of cDNA and PCR amplification was performed using standard protocols described elsewhere (Sambrook J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual., 2nd Ed. Cold Spring Harbor Laboratory Press, New York.), and are basic for those skilled in the art. PCR products were purified using PCR purification kit (Qiagen) and sequencing of the amplified PCR products was performed, using ABI 377 sequencer (Applied Biosystems).

Usually, 2 sets of primers were ordered for the amplification of each gene, via nested PCR (meaning first amplifying the gene using external primers and then using the produced PCR product as a template for a second PCR reaction, where the internal set of primers are used). Alternatively, one or two of the internal primers were used for gene amplification, both in the first and the second PCR reactions (meaning only 2-3 primers were designed for a gene). To facilitate further cloning of the cDNAs, a 8-12 bp extension is added to the 5′ primer end of each internal primer. The primer extension includes an endonuclease restriction site. The restriction sites are selected using two parameters: (a) The restriction site does not exist in the cDNA sequence; and (b) The restriction sites in the forward and reverse primers are designed so the digested cDNA is inserted in the sense formation into the binary vector utilized for transformation. In Table 6 below, primers used for cloning tomato and barley AQPs are provided.

TABLE 6 Primers used for cloning tomato and barley AQP genes Forward external Forward internal Reverse external Reverse internal MAB primer sequence primer sequence primer sequence primer sequence gene from 5′→3′ from 5′→3′ from 5′→3′ from 5′→3′ No. (SEQ ID NO:) (SEQ ID NO:) (SEQ ID NO:) (SEQ ID NO:)  55 GGAGTCGACGACCAT GGAGTCGACTT TGAGCTCACTTC TGAGCTCCCATCC CAAGTTTTAAGTGAC AAGTACATTCTT AAAACCATCCG GTTGTCAAAATGA TTC TAGTGAGAGCC TTGTC AC (2770) (2771) (2772) (2773)  56 GGAGTCGACGT CGAGCTCGTAA CGAGCTCAAGAC AAGAAACAATA AGCCAAGTTTTG AAACAAAGAGAA ATGCCAATTTC AAAGAC GAGGG (2774) (2775) (2776)  57 GTTAAAAATGC GCGATATCTAA GCGATATCAGCCG CGATCAACC ATAACAAAAGC TCCGAATAAACAA (2777) CGTCCG AG (2778) (2779)  58 AATGTCGACCGAATT TATGTCGACTTC TTTCTAGAGGTC TTTCTAGAGATGT GATCTCCTTCTTGATC ATTTCTTGGGTC TGGGATTATCGT GCAGGCAGCTAC (2780) ACTCG CTTG ATAC (2781) (2782) (2783)  59 AATGTCGACTTTAAG AATGTCGACTC AATCTAGATTAGA CGGTGTGTTTTGTG ACAATTATGCA CCCAAACATACAA (2784) GCCACG ACTTCAC (2785) (2786))  69 TAAGTCGACACAAAC AATGTCGACCTT TGAGCTCTGGAGA CTTATCCTGGTCTCAT GGATTCCATTGA AAGAAAACTTTAG C GCACTC ATACA (2787) (2788) (2789)  70 AACTGCAGAGCTGTA AACTGCAGTGT TCCCGGGCCAG TCCCGGGCTTCAA CATGGTCCTCCTCC ACATGGTCCTCC ACAAAACTTCA TTTCATCTTCTGA (2790) TCCG ATTTCATC TTTC (2791) (2792) (2793)  71 AAAGTCGACGGAAAA TTTGTCGACCTT AATCTAGACAA AATCTAGAGTACT TGCATTAAAACCTTA AGTTTTCTCCCA GTAGAGGTACT AGGTAGGGACAA AG CATATGG AGGTAGGGAC TATGATATG (2794) (2795) (2796) (2797)  72 AATGTCGACGTGGAG AATGTCGACCTC TATCTAGAGGATG GAGGAGTCTTTGATA CAACACTCTTAT CAACTACAAAGA C CAATTACCA AATTG (2798) (2799) (2800)  74 TTCTGCAGGTTT TCCCGGGGCATAG GGGAGTTATTG TTCACACAGAGCA ATCTAAGATG AATC (2801) (2802)  76 AATGTCGACCTGTAT AATGTCGACGT TTTCTAGACTAG AATCTAGATTAGA CCTCTTAAGTATGAA CGTCTTGTATGT TGGTATAGATC GCTGGAGAATGA TCG ATTTGTACTACT ATTTTATGGTGA ACTGAAGC (2803) G C (2806) (2804) (2805)  77 AACTGCAGCTTCTTTC AACTGCAGCTTT ACCCGGGAATT TCCCGGGTCCAAC ACCGAGTGGGAG CACCGAGTGGG CCAACTAGCTGT TAGCTGTTATGAT (2807) AGAG TATGATTC TCTG (2808) (2809) (2810)  79 AAGATATCAAAAAAA AAGATATCAAC AAGATATCGACCA TGTCGAAGGACGTG AATGTCGAAGG CCAACTCTAGTCT (2811) ACGTGATTGA CATACC (2812) (2813) 115 AGATTCGAATCTTTA AATCTAGAGAA AGAGCTCTTAAGG GCCTG GTCACAGAGAA GAAATTCATCACA (2814) AACAGTCGAG CAAGG (2815) (2816) 116 AAAGTCGACCTCATC TTTGTCGACCAT TATCTAGAATTG TTTCTAGAGACCG AGTGTTAAAGCCATA AAGCCCTCTTTG AATCGAAAGGG TGACACACCATTT AG AGTGTG AAACAC GTAC (2817) (2818) (2819) (2820) 117 TTTCTAGACTCA TGAGCTCCAGATA GCGACAACATT GAGAAGCATGCA TCATCTC TCATC (2821) (2822)

PCR products were purified (PCR Purification Kit, Qiagen, Germany) and digested with the restriction endonucleases (Roche, Switzerland) according to the sites design in the primers (Tables 8 and 9 below). Each of the digested PCR products was cloned first into high copy plasmid pBlue-script KS [Hypertext Transfer Protocol://World Wide Web (dot) stratagene (dot) com/manuals/212205 (dot) pdf] which was digested with the same restriction enzymes. In some cases (Table 8, below) the Nopaline Synthase (NOS) terminator originated from the binary vector pBI101.3 [nucleotide coordinates 4417-4693 in GenBank Accession No. U12640 (SEQ ID NO:2824)] was already cloned into the pBlue-script KS, between the restriction endonuclease sites SacI and EcoRI, so the gene is introduced upstream of the terminator. In other cases (Table 9, below) the At6669 promoter (SEQ ID NO: 2823) is already cloned into the pBlue-script KS, so the gene is introduced downstream of the promoter. The digested PCR products and the linearized plasmid vector were ligated using T4 DNA ligase enzyme (Roche, Switzerland). Sequencing of the inserted genes was conducted, using ABI 377 sequencer (Applied Biosystems). Sequences of few of the cloned AQP genes, as well as their encoded proteins are listed in Table 7, below.

TABLE 7 Cloned sequences Serial Polynucleotide Polypeptide No Gene Name SEQ ID NO: SEQ ID NO: 1 MAB115 2748 2765 2 MAB116 2749 47 3 MAB117 2750 48 4 MAB54 2751 27 5 MAB55 2752 28 6 MAB56 2753 29 7 MAB57 2754 30 8 MAB58 2755 2766 9 MAB59 2756 2767 10 MAB69 2757 33 11 MAB70 2758 34 12 MAB71 2759 2768 13 MAB72 2760 2769 14 MAB72 2843 2769 (Optimized for expression in Arabidopsis,Tomato and Maize) 15 MAB74 2761 38 16 MAB76 2762 40 17 MAB77 2763 41 18 MAB79 2764 43 Table 7.

The genes were digested again and ligated into pPI or pGI binary plasmids, harboring the At6669 promoter (between the HindIII and SalI restriction endonucleases site) (Table 8). In other cases the At6669 promoter together with the gene are digested out of the pBlue-script KS plasmid and ligated into pPI or pGI binary plasmids, using restriction endonucleases as given in Table 8.

TABLE 8 Restriction enzyme sites used to clone the MAB AQP genes into pKS + NOS terminator high copy plasmid, followed by cloning into the binary vector pGI + At6669 promoter Restriction Restriction enzymes used enzymes used Restriction Restriction Restriction MAB for cloning for cloning into enzymes used for enzymes used enzymes used gene No. into high copy high copy cloning into for cloning into for digesting (SEQ ID plasmid- plasmid- binary vector- binary vector- the binary NO:) FORWARD REVERSE FORWARD REVERSE vector 54 (SEQ XbaI SacI SalI EcoRI SalI/EcoRI ID NO: 2751) 55 (SEQ SalI SacI SalI EcoRI SalI/EcoRI ID NO: 2752) 56 (SEQ SalI SacI SalI EcoRI SalI/EcoRI ID NO: 2753) 58 (SEQ SalI XbaI SalI EcoRI SalI/EcoRI ID NO: 2755) 59 (SEQ SalI XbaI SalI EcoRI SalI/EcoRI ID NO: 2756) 69 (SEQ SalI SacI SalI EcoRI SalI/EcoRI ID NO: 2757) 71 (SEQ SalI XbaI SalI EcoRI SalI/EcoRI ID NO: 2759) 72 (SEQ SalI XbaI SalI EcoRI SalI/EcoRI ID NO: 2760) 76 (SEQ SalI XbaI SalI EcoRI SalI/EcoRI ID NO: 2762) 115 XbaI SacI SalI EcoRI SalI/EcoRI (SEQ ID NO: 2748) 116 SalI XbaI SalI EcoRI SalI/EcoRI (SEQ ID NO: 2749) 117 XbaI SacI SalI EcoRI SalI/EcoRI (SEQ ID NO: 2750) Table 8: MAB AQP genes cloned into pKS + NOS terminator high copy plasmid, followed by cloning into the binary vector pGI + At6669 promoter.

TABLE 9 Restriction enzyme sites used to clone the MAB AQP genes into pKS + At6669 promoter high copy plasmid, followed by cloning promoter + gene into pGI binary vector Restriction Restriction Restriction enzymes used enzymes used Restriction enzymes used for cloning for cloning into enzymes used for for cloning Restriction MAB gene into high copy high copy cloning into into binary enzymes used No. (SEQ plasmid- plasmid- binary vector- vector- for digesting the ID NO:) FORWARD REVERSE FORWARD REVERSE binary vector 57 (SEQ Blunt EcoRV SalI EcoRV SalI/Ecl136 II ID NO: 2754) 70 (SEQ PstI SmaI BamHI SmaI BamHI/ ID Ecl136II NO: 2758) 74 (SEQ PstI SmaI BamHI SmaI BamHI/ ID Ecl136II NO: 2761) 77 (SEQ PstI SmaI BamHI SmaI BamHI/ ID Ecl136II NO: 2763) 79 (SEQ EcoRI EcoRV SalI SmaI SalI/Ecl136 II ID NO: 2764) Table 9: MAB AQP genes cloned into pKS + At6669 promoter high copy plasmid, followed by cloning promoter + gene into pGI binary vector.

The pPI plasmid vector was constructed by inserting a synthetic poly-(A) signal sequence, originating from pGL3 basic plasmid vector (Promega, Acc. No. U47295; by 4658-4811) into the HindIII restriction site of the binary vector pBI101.3 (Clontech, Acc. No. U12640). pGI (FIG. 1) is similar to pPI, but the original gene in the back bone is GUS-Intron, rather than GUS. The cloned genes were sequenced.

Synthetic sequences (such as of MAB54, nucleotide SEQ ID NO: 2751, which encodes protein SEQ ID NO: 27; or MAB72 SEQ ID NO:2843, which encodes SEQ ID NO:2769) of some of the cloned polynucleotides were ordered from a commercial supplier (GeneArt, GmbH). To optimize the coding sequence, codon-usage Tables calculated from plant transcriptomes were used [example of such Tables can be found in the Codon Usage Database available online at Hypertext Transfer Protocol://World Wide Web (dot) kazusa (dot) or (dot) jp/codon/]. The optimized coding sequences were designed in a way that no changes were introduced in the encoded amino acid sequence while using codons preferred for expression in dicotyledonous plants mainly tomato and Arabidopsis; and monocotyledonous plants such as maize. Such optimized sequences promote better translation rate and therefore higher protein expression levels. To the optimized sequences flanking additional unique restriction enzymes sites were added to facilitate cloning genes in binary vectors.

Promoters used: CaMV 35S promoter (SEQ ID NO: 2825) and Arabidopsis At6669 promoter (SEQ ID NO: 2823; which is SEQ ID NO:61 of WO04081173 to Evogene Ltd.).

Example 4 Generation of Transgenic Plants Expressing the AQP Genes

Experimental Results

Arabidopsis Transformation—

Arabidopsis transformation of the following MAB genes and orthologues: MAB115, MAB54, MAB55, MAB56, MAB57, MAB58, MAB59 (ortholog of MAB58), MAB69, MAB70, MAB71, MAB72, MAB74, MAB76, MAB77, MAB79, MAB116 (ortholog of MAB115 and MAB55), and MAB117 (the sequence identifiers of the cloned polynucleotides and their expressed polypeptides are provided in Table 7 above) was performed according to Clough S J, Bent A F. (1998) “Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.” Plant J. 16(6): 735-43; and Desfeux C, Clough S J, Bent A F. (2000) “Female reproductive tissues are the primary targets of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method.” Plant Physiol. 123(3): 895-904; with minor modifications. Briefly, Arabidopsis thaliana Columbia (Col0) T₀ Plants were sown in 250 ml pots filled with wet peat-based growth mix. The pots were covered with aluminum foil and a plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubated in a growth chamber at 18-24° C. under 16/8 hours light/dark cycles. The T₀ plants were ready for transformation six days prior to anthesis. Single colonies of Agrobacterium carrying the binary vectors harboring the AQP genes are cultured in LB medium supplemented with kanamycin (50 mg/L) and gentamycin (50 mg/L). The cultures were incubated at 28° C. for 48 hours under vigorous shaking and centrifuged at 4000 rpm for 5 minutes. The pellets comprising Agrobacterium cells were resuspended in a transformation medium which contained half-strength (2.15 g/L) Murashige-Skoog (Duchefa); 0.044 μM benzylamino purine (Sigma); 112 μg/L B5 Gambourg vitamins (Sigma); 5% sucrose; and 0.2 ml/L Silwet L-77 (OSI Specialists, Conn.) in double-distilled water, at pH of 5.7.

Transformation of T₀ plants was performed by inverting each plant into an Agrobacterium suspension such that the flowering stem was submerged for 3-5 seconds. Each inoculated T₀ plant was immediately placed in a plastic tray, then covered with clear plastic dome to maintain humidity and kept in the dark at room temperature for 18 hours to facilitate infection and transformation. Transformed (transgenic) plants were then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic T₀ plants were grown in the greenhouse for 3-5 weeks until siliques maturation, and then seeds were harvested and kept at room temperature until sowing.

For generating T1 and T₂ transgenic plants harboring the genes, seeds collected from transgenic T₀ plants are surface-sterilized by soaking in 70% ethanol for 1 minute, followed by soaking in 5% sodium hypochlorite and 0.05% Triton X-100 for 5 minutes. The surface-sterilized seeds are thoroughly washed in sterile distilled water then placed on culture plates containing half-strength Murashige-Skoog (Duchefa); 2% sucrose; 0.8% plant agar; 50 mM kanamycin; and 200 mM carbenicylin (Duchefa). The culture plates are incubated at 4° C. for 48 hours then transferred to a growth room at 25° C. for an additional week of incubation. Vital T₁ Arabidopsis plants are transferred to fresh culture plates for another week of incubation. Following incubation the T₁ plants are removed from culture plates and planted in growth mix contained in 250 ml pots. The transgenic plants were allowed to grow in a greenhouse to maturity. Seeds harvested from T₁ plants are cultured and grown to maturity as T₂ plants under the same conditions as used for culturing and growing the T₁ plants. At least 10 independent transformation events are created from each construct for which T2 seeds are collected. The introduction of the gene is determined for each event by PCR performed on genomic DNA extracted from each event produced.

Transformation of Tomato (Var M82) Plants with Putative Cotton Genes—

Tomato (Solanum esculentum, var M82) transformation and cultivation of transgenic plants is effected according to: “Curtis I. S, Davey M. R, and Power J. B. 1995. “Leaf disk transformation”. Methods Mol. Biol. 44, 59-70 and Meissner R, Chague V, Zhu Q, Emmanuel E, Elkind Y, Levy A. A. 2000. “Technical advance: a high throughput system for transposon tagging and promoter trapping in tomato”. Plant J. 22, 265-74; with slight modifications.

Example 5 Evaluating Transgenic Arabidopsis Plant Growth Under Abiotic Stress as Well as Under Favorable Conditions in Tissue Culture Assay

Assay 1: Plant Growth Under Osmotic Stress [Poly (Ethylene Glycol) (PEG)] in Tissue Culture Conditions—

One of the consequences of drought is the induction of osmotic stress in the area surrounding the roots; therefore, in many scientific studies, PEG (e.g., 25% PEG8000) is used to simulate the osmotic stress conditions resembling the high osmolarity found during drought stress.

Surface sterilized seeds were sown in basal media [50% Murashige-Skoog medium (MS) supplemented with 0.8% plant agar as solidifying agent] in the presence of Kanamycin (for selecting only transgenic plants). After sowing, plates were transferred for 2-3 days for stratification at 4° C. and then grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7 to 10 days. At this time point, seedlings randomly chosen were carefully transferred to plates containing 25% PEG: 0.5 MS media or Normal growth conditions (0.5 MS media). Each plate contained 5 seedlings of the same transgenic event, and 3-4 different plates (replicates) for each event. For each polynucleotide of the invention at least four independent transformation events were analyzed from each construct. Plants expressing the polynucleotides of the invention were compared to the average measurement of the control plants (empty vector or GUS reporter gene under the same promoter) used in the same experiment.

Digital Imaging—

A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4×150 Watts light bulb) and located in a darkroom, was used for capturing images of plantlets sawn in agar plates.

The image capturing process was repeated every 2-5 days starting at day 1 till day 10-15 (see for example the images in FIGS. 2A-B)

An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39 (Java based image processing program which was developed at the U.S. National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/). Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Seedling Analysis—

Using the digital analysis seedling data was calculated, including leaf area, root coverage and root length.

The relative growth rate for the various seedling parameters was calculated according to the following formulas II, III and IV. Relative growth rate of leaf area=(Δ rosette area/Δt)*(1/rosette area t ₁)  Formula II

Δ rosette area is the interval between the current rosette area (measured at t₂) and the rosette area measured at the previous day (Area t₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzed image day (t₂) and the previous day (t₁).

Thus, the relative growth rate of leaf area is in units of 1/day. Relative growth rate of root coverage=(Δ root coverage area/Δt)*(1/root coverage area t ₁)  Formula III

Δ root coverage area is the interval between the current root coverage area (measured at t₂) and the root coverage area measured at the previous day (Area t₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzed image day (t₂) and the previous day (t₁).

Thus, the relative growth rate of root coverage area is in units of 1/day. Relative growth rate of root length=(Δ root length/Δt)*(1/root length t ₁)  Formula IV

Δ root length is the interval between the current root length (measured at t₂) and the root length measured at the previous day (Area t₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzed image day (t₂) and the previous day (t₁).

Thus, the relative growth rate of root length is in units of 1/day.

At the end of the experiment, plantlets were removed from the media and weighed for the determination of plant fresh weight. Plantlets were then dried for 24 hours at 60° C., and weighed again to measure plant dry weight for later statistical analysis. Growth rate was determined by comparing the leaf area coverage, root coverage and root length, between each couple of sequential photographs, and results were used to resolve the effect of the gene introduced on plant vigor, under osmotic stress, as well as under optimal conditions. Similarly, the effect of the gene introduced on biomass accumulation, under osmotic stress as well as under optimal conditions, was determined by comparing the plants' fresh and dry weight to that of control plants (containing an empty vector or the GUS reporter gene under the same promoter). From every construct created, 3-5 independent transformation events were examined in replicates.

Statistical Analyses—

To identify genes conferring significantly improved tolerance to abiotic stresses or enlarged root architecture, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. To evaluate the effect of a gene event over a control the data was analyzed by Student's t-test and the p value was calculated. Results were considered significant if p≦0.1. The JMP statistics software package was used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results—

The polynucleotide sequences of the invention were assayed for a number of desired traits.

Tables 10-14 depict analyses of the above mentioned growth parameters of seedlings overexpres sing the polynucleotides of the invention under the regulation of the At6669 promoter (SEQ ID NO:2823) when grown under osmotic stress (25% PEG) conditions.

TABLE 10 MAB70 - 25% PEG Event No. 7971.3 7972.1 7974.1 Control A P A P A P RGR of Roots 0.16 0.29 0.00 0.31 0.00 0.30 0.02 Coverage between day 5 and 10 RGR of Roots 0.07 0.12 0.05 0.13 0.03 Length between day 1 and 5 Table 10: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25% PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 11 MAB76 - 25% PEG Event No. 7635.4 7635.1 Control A P A P RGR of Roots Coverage between 0.16 0.22 0.02 0.21 0.09 day 5 and 10 Table 11: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25% PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 12 MAB79 - 25% PEG Event No. 7324.1 7961.1 Control A P A P Dry Weight [gr] 0.01 0.01 0.06 Fresh Wight [gr] 0.08 0.13 0.07 Leaf Area on day 5 0.15 0.19 0.05 RGR of Roots Coverage between 0.16 0.32 0.05 day 5 and 10 RGR of Roots Length between 0.07 0.11 0.02 day 1 and 5 Table 12: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25% PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 13 MAB56 - 25% PEG Event No. 6802.10 Control A P Roots Coverage on day 7 2.46 3.38 0.09 Roots Length on day 7 2.81 3.43 0.07 Table 13: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25% PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 14 MAB58 - 25% PEG Event No. 6783.30 Control A P Leaf Area on day 7 0.31 0.41 0.03 Leaf Area on day 14 0.80 1.09 0.02 Fresh Weight 0.18 0.31 0.00 Dry Weight [gr] 0.01 0.013 0.01 Table 14: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25% PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Tables 15-29 depict analyses of the above mentioned growth parameters of seedlings overexpressing the polynucleotides of the invention under the regulation of the At6669 promoter in Normal Growth conditions (0.5 MS medium).

TABLE 15 MAB70 - Normal Growth Conditions Event No. 7971.3 7972.1 7974.1 7974.3 Control A P A P A P A P Dry Weight [gr] 0.01 0.01 0.03 0.01 0.05 0.02 0.04 0.01 0.07 Fresh Wight [gr] 0.14 0.24 0.04 0.22 0.10 Leaf Area on day 10 0.46 0.59 0.00 Leaf Area on day 5 0.21 0.30 0.10 RGR of Roots Coverage 0.18 0.42 0.00 0.37 0.00 0.32 0.01 between day 5 and 10 RGR of Roots Length 0.06 0.15 0.01 0.16 0.00 0.15 0.00 between day 1 and 5 RGR of Roots Length 0.22 0.32 0.09 between day 5 and 10 Table 15: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 16 MAB71 - Normal Growth Conditions Event No. 7331.5 7332.2 7333.5 Control A P A P A P Dry Weight [gr] 0.01 0.01 0.05 Fresh Wight [gr] 0.14 0.20 0.08 RGR of Roots 0.18 0.28 0.04 0.26 0.07 Coverage between day 5 and 10 RGR of Roots 0.06 0.11 0.02 0.11 0.01 Length between day 1 and 5 Table 16: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 17 MAB74 - Normal Growth Conditions Event No. 7981.1 7982.4 7983.9 Control A P A P A P Dry Weight [gr] 0.01 0.01 0.09 0.02 0.05 0.01 0.06 Fresh Wight [gr] 0.14 0.21 0.01 Leaf Area on day 10 0.46 0.67 0.01 Leaf Area on day 5 0.21 0.29 0.04 0.29 0.01 0.27 0.00 RGR of Roots 0.18 0.31 0.07 Coverage between day 5 and 10 RGR of Roots 0.73 1.70 0.07 Coverage between day 1 and 5 RGR of Roots 0.06 0.12 0.09 0.14 0.03 Length between day 1 and 5 RGR of Roots 0.22 0.45 0.05 0.58 0.00 Length between day 5 and 10 Table 17: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 18 MAB76 - Normal Growth Conditions Event No. 7633.3 7635.4 7635.1 Control A P A P A P RGR Leaf Area 0.24 0.34 0.04 0.33 0.07 between day 5 and 10 RGR of Roots 0.18 0.38 0.08 Coverage between day 5 and 10 RGR of Roots 0.06 0.13 0.02 Length between day 1 and 5 Table 18: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 19 MAB77 - Normal Growth Conditions Event No. 7931.11 8211.2 8212.2 Control A P A P A P Dry Weight [gr] 0.01 0.01 0.08 0.01 0.10 Roots Coverage on 2.77 4.33 0.07 day 10 RGR Leaf Area 0.24 0.38 0.10 0.35 0.04 between day 5 and 10 RGR of Roots 0.18 0.27 0.09 0.29 0.09 Coverage between day 5 and 10 RGR of Roots 0.06 0.10 0.03 Length between day 1 and 5 Table 19: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 20 MAB79 - Normal Growth Conditions Event No. 7323.3 7324.1 7961.1 7962.2 Control A P A P A P A P Dry Weight [gr] 0.01 0.02 0.03 0.02 0.08 0.01 0.00 Fresh Wight [gr] 0.14 0.34 0.04 0.31 0.09 Leaf Area on day 10 0.46 0.92 0.01 0.90 0.01 0.81 0.00 Leaf Area on day 5 0.21 0.29 0.02 0.28 0.00 Roots Coverage on day 10 2.77 5.31 0.02 4.59 0.07 3.81 0.00 3.71 0.01 RGR Leaf Area between day 5 and 10 0.24 0.36 0.02 RGR Leaf Area between day 1 and 5 0.82 1.34 0.00 RGR of Roots Coverage between day 5 0.18 0.45 0.06 0.39 0.02 and 10 RGR of Roots Length between day 1 and 5 0.06 0.12 0.02 0.17 0.06 0.14 0.00 Table 20: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 21 MAB115 - Normal Growth Conditions Event No. 8561.2 . . . 8564.1 . . . 8564.2 . . . 8565.1 . . . Control A P A P A P A P Dry Weight [gr] 0.005 0.006 0.00 0.009 0.00 Leaf Area on day 10 0.24 0.27 0.00 Leaf Area on day 5 0.35 0.38 0.01 Roots Coverage on day 10 1.67 2.13 0.02 Roots Coverage on day 5 3.38 4.80 0.03 Roots Length on day 10 2.39 2.74 0.00 Roots Length on day 5 3.46 4.22 0.02 RGR Leaf Area between day 5 and 10 0.37 0.43 0.00 0.48 0.00 RGR Leaf Area between day 1 and 5 0.16 0.19 0.00 RGR of Roots Coverage between day 5 1.89 3.21 0.00 2.24 0.02 2.23 0.02 3.47 0.01 and 10 RGR of Roots Coverage between day 1 0.33 0.35 0.00 0.41 0.00 0.43 0.00 0.44 0.00 and 5 RGR of Roots Length between day 1 0.37 0.56 0.02 0.53 0.06 0.68 0.00 and 5 RGR of Roots Length between day 5 0.15 0.18 0.00 0.17 0.00 0.18 0.00 and 10 Table 21: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 22 MAB54 - Normal Growth Conditions Event No. 8181.2 . . . 8182.2 . . . 8184.3 . . . 8185.3 . . . Control A P A P A P A P Dry Weight [gr] 0.005 0.009 0.00 0.008 0.00 0.008 0.00 0.007 0.00 Roots Coverage on day 10 1.67 1.80 0.04 Roots Coverage on day 5 3.38 4.27 0.02 3.40 0.00 Roots Length on day 10 2.39 2.52 0.01 Roots Length on day 5 3.46 4.11 0.02 3.50 0.00 RGR Leaf Area between day 5 and 10 0.37 0.45 0.00 RGR Leaf Area between day 1 and 5 0.16 0.17 0.04 0.19 0.00 RGR of Roots Coverage between day 1.89 3.59 0.00 3.60 0.01 2.28 0.01 2.20 0.01 5 and 10 RGR of Roots Coverage between day 0.33 0.52 0.00 0.55 0.00 0.39 0.00 0.36 0.00 1 and 5 RGR of Roots Length between day 1 0.37 0.70 0.00 0.73 0.00 0.48 0.08 0.51 0.02 and 5 RGR of Roots Length between day 5 0.15 0.21 0.01 0.22 0.01 0.17 0.00 0.17 0.00 and 10 Table 22: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 23 MAB55 - Normal Growth Conditions Event No. 6802.1 6802.11 6802.8 6805.3 A P A P A P A P Roots Coverage on day 7 3.67 5.93 0.04 Roots Coverage on day 14 7.40 13.26 0.04 Roots Length on day 7 3.99 5.32 0.01 Roots Length on day 14 6.14 7.80 0.04 7.65 0.04 RGR of Roots Coverage between day 1 and 7 0.53 1.30 0.00 1.11 0.02 0.93 0.02 RGR of Roots Length between day 1 and 7 0.29 0.48 0.00 0.45 0.03 0.43 0.02 Fresh Weight 0.19 0.10 0.00 0.12 0.01 Dry Weight [gr] 0.01 0.01 0.00 0.01 0.00 Table 23: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 24 MAB56 - Normal Growth Conditions Event No. 6691.2 6691.3 6693.2 6693.5 A P A P A P A P Roots Coverage on day 7 3.67 5.81 0.00 5.67 0.01 Roots Length on day 7 3.99 6.21 0.00 5.39 0.00 Roots Length on day 14 6.14 8.32 0.01 8.01 0.02 RGR of Roots Length 0.09 0.05 0.01 0.05 0.06 0.06 0.08 between day 7 and 14 Fresh Weight 0.19 0.14 0.03 0.14 0.03 Dry Weight [gr] 0.01 0.01 0.02 0.01 0.02 0.00 0.00 Table 24: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 25 MAB57 - Normal Growth Conditions Event No. 6912.14 6912.2 6912.6 6914.1 A P A P A P A P Roots Coverage on day 7 3.67 6.40 0.00 6.71 0.00 Roots Coverage on day 14 7.40 17.33 0.00 15.27 0.05 12.30 0.02 Roots Length on day 7 3.99 5.54 0.00 6.12 0.00 6.34 0.00 Roots Length on day 14 6.14 8.76 0.00 8.48 0.01 7.97 0.02 7.85 0.04 RGR of Roots Length 0.29 0.39 0.06 between day 1 and 7 RGR of Roots Length 0.09 0.04 0.09 between day 7 and 14 Fresh Weight 0.19 0.24 0.09 0.11 0.01 0.11 0.01 Dry Weight [gr] 0.01 0.01 0.01 0.01 0.01 Table 25: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 26 MAB58 - Normal Growth Conditions Event No. 6783.1 6783.2 6783.3 A P A P A P Roots Coverage on 3.67 6.18 0.00 day 7 Roots Coverage 7.40 12.65 0.02 12.00 0.09 on day 14 Roots Length on day 7 3.99 5.20 0.02 Roots Length on 6.14 7.38 0.09 7.51 0.07 7.59 0.08 day 14 RGR of Roots 0.29 0.35 0.07 Length between day 1 and 7 Fresh Weight 0.19 0.13 0.03 Dry Weight [gr] 0.01 0.01 0.00 Table 26: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 27 MAB59 - Normal Growth Condition Event No. 6791.4 6793.4 6794.4 A P A P A P Roots Coverage on 3.67 5.61 0.04 5.23 0.09 day 7 Roots Coverage on 7.40 9.65 0.09 10.28 0.09 day 14 Roots Length on day 7 3.99 5.47 0.08 4.95 0.09 Roots Length on 6.14 7.70 0.07 day 14 RGR of Roots 0.53 0.80 0.09 Coverage between day 1 and 7 RGR of Roots Length 0.09 0.05 0.10 between day 7 and 14 Fresh Weight 0.19 0.09 0.00 Dry Weight [gr] 0.01 0.004 0.00 0.005 0.02 Table 27: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 28 MAB69 - Normal Growth Conditions Event No. 6651.1 6651.12 6651.13 6651.8 A P A P A P A P Roots Length on day 7 3.99 4.97 0.10 Roots Length on day 14 6.14 8.01 0.02 RGR of Roots Length 0.29 0.35 0.09 between day 1 and 7 Fresh Weight 0.19 0.12 0.02 0.12 0.01 Dry Weight [gr] 0.01 0.007 0.09 0.005 0.00 0.006 0.00 Table 28: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 29 MAB72 - Normal Growth Conditions Event No. 8552.1 . . . 8552.4 . . . 8553.2 . . . Control A P A P A P Dry Weight [gr] 0.005 0.008 0.00 0.008 0.00 0.007 0.00 Leaf Area on day 10 0.24 0.24 0.01 Roots Coverage on day 10 1.67 1.84 0.01 1.93 0.02 1.89 0.03 Roots Coverage on day 5 3.38 3.60 0.04 3.90 0.06 4.50 0.00 Roots Length on day 10 2.39 2.48 0.01 Roots Length on day 5 3.46 3.70 0.04 3.65 0.04 3.84 0.00 RGR Leaf Area between 0.37 0.47 0.00 0.39 0.00 day 5 and 10 RGR Leaf Area between 0.16 0.20 0.09 day 1 and 5 RGR of Roots Coverage 1.89 1.93 0.03 2.29 0.06 3.04 0.01 between day 5 and 10 RGR of Roots Coverage 0.33 0.35 0.00 0.52 0.00 between day 1 and 5 RGR of Roots Length 0.37 0.55 0.01 between day 1 and 5 RGR of Roots Length 0.15 0.16 0.00 0.18 0.00 0.22 0.01 between day 5 and 10 Table 29: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

Example 6 Evaluating Transgenic Arabidopsis Plant Growth Under Abiotic Stress as Well as Favorable Conditions in Greenhouse Assay

ABS Tolerance: Yield and Plant Growth Rate at High Salinity Concentration Under Greenhouse Conditions—

This assay followed the rosette area growth of plants grown in the greenhouse as well as seed yield at high salinity irrigation. Seeds were sown in agar media supplemented only with a selection agent (Kanamycin) and Hoagland solution under nursery conditions. The T₂ transgenic seedlings were then transplanted to 1.7 trays filled with peat and perlite. The trays were irrigated with tap water (provided from the pots' bottom). Half of the plants were irrigated with a salt solution (40-80 mM NaCl and 5 mM CaCl₂) so as to induce salinity stress (stress conditions). The other half of the plants was irrigated with tap water (normal conditions). All plants were grown in the greenhouse until mature seeds, then harvested (the above ground tissue) and weighted (immediately or following drying in oven at 50° C. for 24 hours). High salinity conditions were achieved by irrigating with a solution containing 40-80 mM NaCl (“ABS” growth conditions) and compared to regular growth conditions.

Each construct was validated at its T2 generation. Transgenic plants transformed with a construct including the uidA reporter gene (GUS) under the AT6669 promoter or with an empty vector including the AT6669 promoter were used as control.

The plants were analyzed for their overall size, growth rate, flowering, seed yield, weight of 1,000 seeds, dry matter and harvest index (HI—seed yield/dry matter). Transgenic plants performance was compared to control plants grown in parallel under the same conditions. Mock-transgenic plants expressing the uidA reporter gene (GUS-Intron) or with no gene at all, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. For each gene of the invention three to five independent transformation events were analyzed from each construct.

Digital Imaging—

A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4×150 Watts light bulb) was used for capturing images of plant samples.

The image capturing process was repeated every 2 days starting from day 1 after transplanting till day 16. Same camera, placed in a custom made iron mount, was used for capturing images of larger plants sawn in white tubs in an environmental controlled greenhouse. The tubs were square shape include 1.7 liter trays. During the capture process, the tubs were placed beneath the iron mount, while avoiding direct sun light and casting of shadows.

An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program—1.39 (Java based image processing program which was developed at the U.S National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/). Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute).

Leaf Analysis—

Using the digital analysis leaves data was calculated, including leaf number, area, perimeter, length and width.

Vegetative Growth Rate:

the relative growth rate (RGR) of leaf number and rosette area were calculated formulas V and VI, respectively. Relative growth rate of leaf number=(Δ leaf number/Δt)*(1/leaf number t ₁)  Formula V

Δ leaf number is the interval between the current leaf number (measured at t₂) and the leaf number measured at the previous day (Area t₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzed image day (t₂) and the previous day (t₁).

Thus, the relative growth rate of leaf number is in units of 1/day. Relative growth rate of rosette area=(Δ rosette area/Δt)*(1/rosette area t ₁)  Formula VI

Δ rosette area is the interval between the current rosette area (measured at t₂) and the rosette area measured at the previous day (Area t₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzed image day (t₂) and the previous day (t₁).

Thus, the relative growth rate of rosette area is in units of 1/day.

Seeds Average Weight—

At the end of the experiment all seeds were collected. The seeds were scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample was calculated.

Dry Weight and Seed Yield—

On about day 80 from sowing, the plants were harvested and left to dry at 30° C. in a drying chamber. The biomass and seed weight of each plot were measured and divided by the number of plants in each plot. Dry weight=total weight of the vegetative portion above ground (excluding roots) after drying at 30° C. in a drying chamber; Seed yield per plant=total seed weight per plant (gr). 1000 seed weight (the weight of 1000 seeds) (gr.).

Harvest Index (HI)—

The harvest index was calculated using Formula VII. Harvest Index=Average seed yield per plant/Average dry weight  Formula VII

Statistical Analyses—

To identify genes conferring significantly improved tolerance to abiotic stresses, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. Data was analyzed using Student's t-test and results were considered significant if the p value was less than 0.1. The JMP statistics software package is used (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results

Tables 30-44 depict analyses of plant parameters as describe above overexpressing the polynucleotides of the invention under the regulation of the At6669 promoter under salinity irrigation conditions [NaCl 40-80 mM; NaCl Electrical conductivity (E.C.) of 7-10].

TABLE 30 MAB115 - Salt irrigation (40-80 mM NaCl) Event No. 8564.1 8565.1 Control A P A P Rosette Diameter on day 3* 1.70 1.80 0.09 1.75 0.04 Rosette Diameter on day 5 2.36 Rosette Diameter on day 8 3.77 4.00 0.09 Rosette Area on day 3 0.90 Rosette Area on day 5 1.56 1.70 0.09 Rosette Area on day 8 4.06 4.38 0.06 Plot Coverage on day 5 12.30 13.61 0.06 Plot Coverage on day 8 31.90 35.07 0.02 Leaf Number on day 3 5.08 5.94 0.00 Leaf Number on day 5 6.86 7.38 0.05 RGR of Leaf Number between 0.18 day 3 and 5 RGR of Leaf Number between 0.09 day 5 and 8 RGR of Rosette Area between 0.53 0.62 0.01 day 5 and 8 Biomass DW [gr] 3.24 Harvest Index 0.11 0.15 0.07 0.16 0.03 Table 30: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 31 MAB54 - Salt irrigation (40-80 mM NaCl) Event No. 8181.2 8182.2 8183.2 8185.4 Control A P A P A P A P Rosette Diameter on day 3* 1.70 1.95 0.04 Rosette Diameter on day 5 2.36 2.70 0.02 2.64 0.00 Rosette Diameter on day 8 3.77 4.00 0.08 Rosette Area on day 3 0.90 1.19 0.04 Plot Coverage on day 3 7.04 9.52 0.04 7.49 0.08 Leaf Number on day 3 5.08 6.19 0.06 Leaf Number on day 8 8.66 9.31 0.00 9.38 0.00 RGR of Leaf Number 0.18 between day 3 and 5 RGR of Rosette Area 0.45 0.52 0.01 between day 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.00 0.02 0.00 Yield [gr]/Plant 0.04 0.06 0.05 Harvest Index 0.11 0.17 0.01 Table 31: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 32 MAB55 - Salt irrigation (40-80 mM NaCl) Event No. 6802.10 6805.3 6805.4 Control A P A P A P Rosette Diameter on day 5 2.36 2.81 0.03 Rosette Diameter on day 8 3.77 4.29 0.05 Rosette Area on day 3 0.90 1.35 0.01 Rosette Area on day 5 1.56 1.70 0.03 Rosette Area on day 8 4.06 5.91 0.05 Plot Coverage on day 3 7.04 10.76 0.01 Plot Coverage on day 5 12.30 13.60 0.02 Plot Coverage on day 8 31.90 47.28 0.06 Leaf Number on day 3 5.08 6.25 0.00 Leaf Number on day 5 6.86 7.94 0.03 Leaf Number on day 8 8.66 9.50 0.01 RGR of Rosette Area 0.45 0.51 0.05 between day 1 and 3 Yield [gr]/Plant 0.04 0.06 0.03 Harvest Index 0.11 0.15 0.05 Table 32: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 33 MAB56 - Salt irrigation (40-80 mM NaCl) Event No. 6691.2 6691.3 6693.2 6695.6 Control A P A P A P A P Rosette Diameter on day 3 1.70 1.89 0.05 1.97 0.07 Rosette Diameter on day 5 2.36 2.55 0.09 2.58 0.01 Rosette Area on day 3 0.90 1.06 0.00 1.15 0.02 1.23 0.08 Rosette Area on day 5 1.56 1.94 0.02 1.94 0.03 Rosette Area on day 8 4.06 4.63 0.02 Plot Coverage on day 3 7.04 8.45 0.00 9.21 0.01 9.82 0.07 Plot Coverage on day 5 12.30 15.49 0.01 15.53 0.02 Plot Coverage on day 8 31.90 37.03 0.01 34.82 0.05 Leaf Number on day 3 5.08 5.50 0.00 5.81 0.00 6.00 0.02 Leaf Number on day 5 6.86 7.38 0.01 7.50 0.02 1000 Seeds weight [gr] 0.02 0.02 0.08 Harvest Index 0.11 0.18 0.01 Table 33: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 34 MAB57 - Salt irrigation (40-80 mM NaCl) Event No. 6912.1 6912.13 6914.5 Control A P A P A P Leaf Number on day 3 5.08 5.69 0.00 Leaf Number on day 5 6.86 8.13 0.06 Leaf Number on day 8 8.66 9.56 0.06 RGR of Leaf Number 0.09 0.14 0.01 between day 5 and 8 RGR of Rosette Area 0.53 0.62 0.02 0.58 0.10 between day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.00 Yield [gr]/Plant 0.04 0.06 0.01 0.06 0.03 0.06 0.01 Harvest Index 0.11 0.19 0.06 0.23 0.00 Table 34: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 35 MAB58 - Salt irrigation (40-80 mM NaCl) Event No. 6783.3 7522.10 7522.3 7523.6 Control A P A P A P A P Rosette Diameter on day 3 1.70 2.11 0.00 Rosette Diameter on day 5 2.36 2.57 0.00 Rosette Diameter on day 8 3.77 4.06 0.07 4.54 0.00 Rosette Area on day 3 0.90 1.36 0.00 Rosette Area on day 5 1.56 2.29 0.00 Rosette Area on day 8 4.06 5.98 0.00 Plot Coverage on day 3 7.04 10.90 0.00 Plot Coverage on day 5 12.30 18.32 0.00 Plot Coverage on day 8 31.90 47.88 0.00 Leaf Number on day 3 5.08 5.63 0.06 6.06 0.00 Leaf Number on day 8 8.66 9.25 0.04 9.81 0.04 RGR of Leaf Number 0.09 0.12 0.03 between day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.08 Yield [gr]/Plant 0.04 0.06 0.09 Table 35: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 36 MAB59 - Salt irrigation (40-80 mM NaCl) Event No. 6791.6 6794.4 6794.5 Control A P A P A P Rosette Diameter on day 3 1.70 2.38 0.05 Rosette Diameter on day 5 2.36 2.73 0.06 Rosette Area on day 3 0.90 1.27 0.06 1.65 0.03 Plot Coverage on day 3 7.04 10.15 0.06 13.19 0.03 Leaf Number on day 3 5.08 6.44 0.05 6.00 0.02 6.75 0.01 Leaf Number on day 5 6.86 8.38 0.00 7.69 0.05 8.00 0.07 Leaf Number on day 8 8.66 9.38 0.02 Yield [gr]/Plant 0.04 0.06 0.06 Harvest Index 0.11 0.16 0.04 Table 36: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 37 MAB69 - Salt irrigation (40-80 mM NaCl) Event No. 6651.11 6651.12 6651.2 8342.1 Control A P A P A P A P Rosette Diameter on day 3 1.70 1.92 0.01 Rosette Area on day 3 0.90 1.09 0.00 Rosette Area on day 8 4.06 5.05 0.04 Plot Coverage on day 3 7.04 8.74 0.00 Plot Coverage on day 8 31.90 40.41 0.04 Leaf Number on day 3 5.08 5.69 0.00 Leaf Number on day 8 8.66 8.94 0.08 RGR of Rosette Area 0.45 0.49 0.02 0.51 0.04 between day 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.00 0.02 0.01 Yield [gr]/Plant 0.04 0.05 0.09 0.06 0.02 0.07 0.01 Harvest Index 0.11 0.17 0.09 0.22 0.01 Table 37: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 38 MAB70 - Salt irrigation (40-80 mM NaCl) Event No. 7971.3 7972.1 7972.3 Control A P A P A P Rosette Diameter on day 3 1.70 1.94 0.00 Rosette Diameter on day 5 2.36 2.62 0.04 Rosette Diameter on day 8 3.77 4.40 0.00 Rosette Area on day 3 0.90 1.20 0.07 1.12 0.01 Rosette Area on day 5 1.56 2.06 0.05 1.87 0.00 Rosette Area on day 8 4.06 5.86 0.06 Plot Coverage on day 3 7.04 9.61 0.06 9.00 0.01 Plot Coverage on day 5 12.30 16.46 0.04 14.93 0.00 Plot Coverage on day 8 31.90 46.87 0.06 Leaf Number on day 3 5.08 5.94 0.09 Leaf Number on day 8 8.66 9.31 0.00 9.75 0.09 RGR of Rosette Area 0.53 0.62 0.01 between day 5 and 8 Yield [gr]/Plant 0.04 0.08 0.00 0.07 0.01 Harvest Index 0.11 0.25 0.00 Table 38: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 39 MAB71 - Salt irrigation (40-80 mM NaCl) Event No. 7331.4 7331.5 7333.5 7334.5 Control A P A P A P A P Rosette Diameter on day 5 2.36 3.03 0.00 2.52 0.02 Rosette Diameter on day 8 3.77 5.01 0.05 4.33 0.01 Plot Coverage on day 5 12.30 19.90 0.09 14.25 0.08 Leaf Number on day 3 5.08 6.44 0.05 5.47 0.06 Leaf Number on day 5 6.86 8.13 0.00 7.56 0.00 Leaf Number on day 8 8.66 10.38 0.05 RGR of Leaf Number 0.09 0.12 0.01 between day 5 and 8 RGR of Rosette Area 0.53 0.61 0.03 0.61 0.04 between day 5 and 8 Yield [gr]/Plant 0.04 0.05 0.10 Harvest Index 0.11 0.21 0.06 Table 39: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 40 MAB72 - Salt irrigation (40-80 mM NaCl) Event No. 8552.1 8553.2 8553.3 8555.3 Control A P A P A P A P Rosette Diameter on day 3 1.70 1.90 0.05 1.83 0.00 Rosette Diameter on day 5 2.36 2.67 0.00 2.55 0.01 Rosette Diameter on day 8 3.77 4.06 0.08 4.15 0.10 Rosette Area on day 3 0.90 1.20 0.00 1.02 0.00 1.08 0.02 Rosette Area on day 5 1.56 1.88 0.07 2.00 0.00 1.87 0.00 Rosette Area on day 8 4.06 4.68 0.00 5.05 0.00 4.82 0.00 Plot Coverage on day 3 7.04 9.64 0.00 8.13 0.00 8.67 0.02 Plot Coverage on day 5 12.30 15.05 0.05 16.04 0.00 14.98 0.00 Plot Coverage on day 8 31.90 37.48 0.00 40.39 0.00 38.57 0.00 Leaf Number on day 3 5.08 5.81 0.00 5.88 0.00 5.56 0.00 5.50 0.00 Leaf Number on day 8 8.66 9.38 0.02 RGR of Leaf Number 0.12 0.17 0.01 between day 1 and 3 Table 40: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 41 MAB74 - Salt irrigation (40-80 mM NaCl) Event No. 7982.1 7983.6 7983.9 Control A P A P A P Rosette Diameter on day 3 1.70 2.06 0.10 1.94 0.00 Rosette Diameter on day 5 2.36 2.62 0.06 Rosette Diameter on day 8 3.77 4.05 0.02 Rosette Area on day 3 0.90 1.14 0.02 Rosette Area on day 5 1.56 1.83 0.05 1.85 0.10 Rosette Area on day 8 4.06 4.46 0.03 Plot Coverage on day 3 7.04 9.13 0.02 Plot Coverage on day 5 12.30 14.62 0.03 14.79 0.08 Plot Coverage on day 8 31.90 35.66 0.01 Leaf Number on day 3 5.08 5.75 0.04 5.31 0.07 Leaf Number on day 5 6.86 7.25 0.04 RGR of Rosette Area 0.37 0.42 0.07 between day 3 and 5 1000 Seeds weight [gr] 0.02 0.02 0.02 0.02 0.05 Yield [gr]/Plant 0.04 0.06 0.05 Harvest Index 0.11 0.20 0.06 Table 41: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 42 MAB76 - Salt irrigation (40-80 mM NaCl) Event No. 7633.1 7633.2 Control A P A P Leaf Number on day 3 5.08 5.44 0.02 Leaf Number on day 8 8.66 9.13 0.07 Table 42: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 43 MAB77 - Salt irrigation (40-80 mM NaCl) Event No. 7931.11 8212.2 Control A P A P Leaf Number on day 8 8.66 9.75 0.09 RGR of Rosette Area between 0.45 0.52 0.10 day 1 and 3 Harvest Index 0.11 0.15 0.07 Table 43: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 44 MAB79 - Salt irrigation (40-80 mM NaCl) Event No. 7323.10, 7961.1, 7962.2, 7962.2, Control A P A P A P A P Rosette Diameter on day 3 1.70 1.84 0.10 1.93 0.05 Rosette Diameter on day 5 2.36 2.53 0.01 Rosette Diameter on day 8 3.77 4.32 0.03 4.01 0.04 Rosette Area on day 3 0.90 1.08 0.09 Rosette Area on day 8 4.06 5.29 0.03 4.78 0.05 Plot Coverage on day 3 7.04 8.63 0.08 Plot Coverage on day 8 31.90 42.32 0.03 38.27 0.05 Leaf Number on day 3 5.08 5.63 0.00 5.75 0.04 Leaf Number on day 5 6.86 7.44 0.01 RGR of Leaf Number 0.09 0.11 0.01 between day 5 and 8 RGR of Rosette Area 0.53 0.59 0.01 between day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.00 Harvest Index 0.11 0.19 0.00 Table 44: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

Tables 45-59 depict analyses of plant parameters (as describe above) overexpressing the polynucleotides of the invention under the regulation of the 6669 promoter under Normal Growth conditions [Normal irrigation included NaCl Electrical conductivity (E.C.) of 1-2].

TABLE 45 MAB115 - Normal Growth Conditions Event No. 8564.1 8565.1 8565.2 Control A P A P A P Rosette Diameter on day 3 1.67 1.80 0.09 1.75 0.04 1.95 0.04 Rosette Diameter on day 8 3.60 4.00 0.09 Rosette Area on day 5 1.54 1.70 0.09 Rosette Area on day 8 3.98 4.38 0.06 Plot Coverage on day 5 12.30 13.61 0.06 Plot Coverage on day 8 31.82 35.07 0.02 Leaf Number on day 3 5.25 5.94 0.00 Leaf Number on day 5 6.52 7.38 0.05 Leaf Number on day 8 8.92 9.31 0.00 RGR of Leaf Number 0.12 0.12 0.04 between day 3 and 5 RGR of Rosette Area 0.53 0.62 0.01 between day 5 and 8 Table 45: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 46 MAB54 - Normal Growth Conditions Event No. 8181.2 8182.2 8184.3 8185.4 Control A P A P A P A P Rosette Diameter on day 5 2.24 2.70 0.02 2.64 0.00 2.81 0.03 Rosette Diameter on day 8 3.60 4.00 0.08 4.29 0.05 Rosette Area on day 3 0.89 1.19 0.04 1.35 0.01 Rosette Area on day 8 3.98 5.91 0.05 Plot Coverage on day 3 7.10 9.52 0.04 7.49 0.08 10.76 0.01 Plot Coverage on day 8 31.82 47.28 0.06 Leaf Number on day 3 5.25 6.19 0.06 6.25 0.00 Leaf Number on day 5 6.52 7.94 0.03 Leaf Number on day 8 8.92 9.38 0.00 9.50 0.01 RGR of Leaf Number 0.12 0.13 0.08 between day 3 and 5 RGR of Rosette Area 0.46 0.52 0.01 0.51 0.05 between day 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.00 0.02 0.00 Table 46: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 47 MAB55 - Normal Growth Conditions Event No. 6802.5 6805.4 Control A P A P Rosette Area on day 5 1.54 1.70 0.03 Rosette Area on day 8 3.98 4.63 0.02 Plot Coverage on day 5 12.30 13.60 0.02 Plot Coverage on day 8 31.82 37.03 0.01 Table 47: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 48 MAB56 - Normal Growth Conditions Event No. 6691.2 6691.3 6693.2 6695.7 Control A P A P A P A P Rosette Diameter on day 3 1.67 1.89 0.05 1.97 0.07 Rosette Diameter on day 5 2.24 2.55 0.09 2.58 0.01 Rosette Area on day 3 0.89 1.06 0.00 1.15 0.02 1.23 0.08 Rosette Area on day 5 1.54 1.94 0.02 1.94 0.03 Plot Coverage on day 3 7.10 8.45 0.00 9.21 0.01 9.82 0.07 Plot Coverage on day 5 12.30 15.49 0.01 15.53 0.02 Plot Coverage on day 8 31.82 34.82 0.05 Leaf Number on day 3 5.25 5.50 0.00 5.81 0.00 6.00 0.02 Leaf Number on day 5 6.52 7.38 0.01 7.50 0.02 RGR of Leaf Number 0.12 0.13 0.06 between day 3 and 5 RGR of Leaf Number 0.12 0.14 0.01 between day 5 and 8 RGR of Rosette Area 0.53 0.62 0.02 between day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.08 Table 48: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 49 MAB57 - Normal Growth Conditions Event No. 6912.1 6912.6 6912.9 Control A P A P A P Rosette Area on day 8 3.98 4.48 0.02 Plot Coverage on day 8 31.82 35.81 0.01 Leaf Number on day 3 5.25 5.69 0.00 Leaf Number on day 5 6.52 8.13 0.06 8.13 0.06 Leaf Number on day 8 8.92 9.56 0.06 RGR of Rosette Area 0.53 0.58 0.10 between day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.00 Table 49: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 50 MAB58 - Normal Growth Conditions Event No. 6783.2 7522.10 7522.3 7523.6 Control A P A P A P A P Rosette Diameter on day 3 1.67 2.11 0.00 Rosette Diameter on day 5 2.24 2.57 0.00 Rosette Diameter on day 8 3.60 4.06 0.07 4.54 0.00 Rosette Area on day 3 0.89 1.36 0.00 Rosette Area on day 5 1.54 2.29 0.00 Rosette Area on day 8 3.98 5.98 0.00 Plot Coverage on day 3 7.10 10.90 0.00 Plot Coverage on day 5 12.30 18.32 0.00 Plot Coverage on day 8 31.82 47.88 0.00 Leaf Number on day 3 5.25 6.06 0.00 6.44 0.05 Leaf Number on day 5 6.52 8.38 0.00 Leaf Number on day 8 8.92 9.25 0.04 9.81 0.04 RGR of Leaf Number 0.12 0.12 0.03 between day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.08 Table 50: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 51 MAB59 - Normal Growth Conditions Event No. 6793.4 6794.4 6794.5 Control A P A P A P Rosette Diameter on day 3 1.67 2.38 0.05 Rosette Diameter on day 5 2.24 2.73 0.06 Rosette Area on day 3 0.89 1.27 0.06 1.65 0.03 Plot Coverage on day 3 7.10 10.15 0.06 13.19 0.03 Leaf Number on day 3 5.25 6.00 0.02 6.75 0.01 Leaf Number on day 5 6.52 7.69 0.05 8.00 0.07 Leaf Number on day 8 8.92 9.38 0.02 RGR of Rosette Area 0.46 0.49 0.02 between day 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.00 Table 51: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 52 MAB69 - Normal Growth Conditions Event No. 6651.11 6651.12 8341.1 8342.1 Control A P A P A P A P Rosette Diameter on day 3 1.67 1.92 0.01 Rosette Area on day 3 0.89 1.09 0.00 Rosette Area on day 8 3.98 5.05 0.04 Plot Coverage on day 3 7.10 8.74 0.00 Plot Coverage on day 8 31.82 40.41 0.04 Leaf Number on day 3 5.25 5.69 0.00 5.94 0.09 Leaf Number on day 8 8.92 8.94 0.08 9.31 0.00 RGR of Rosette Area 0.46 0.51 0.04 between day 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.01 Table 52: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 53 MAB70 - Normal Growth Conditions Event No. 7971.3 7972.1 7974.3 Control A P A P A P Rosette Diameter on day 3 1.67 1.94 0.00 2.27 0.00 Rosette Diameter on day 5 2.24 2.62 0.04 3.03 0.00 Rosette Diameter on day 8 3.60 4.40 0.00 5.01 0.05 Rosette Area on day 3 0.89 1.20 0.07 1.12 0.01 1.52 0.04 Rosette Area on day 5 1.54 2.06 0.05 1.87 0.00 2.49 0.10 Rosette Area on day 8 3.98 5.86 0.06 7.03 0.05 Plot Coverage on day 3 7.10 9.61 0.06 9.00 0.01 12.12 0.04 Plot Coverage on day 5 12.30 16.46 0.04 14.93 0.00 19.90 0.09 Plot Coverage on day 8 31.82 46.87 0.06 56.23 0.05 Leaf Number on day 3 5.25 6.44 0.05 Leaf Number on day 5 6.52 8.13 0.00 Leaf Number on day 8 8.92 9.75 0.09 10.38 0.05 RGR of Rosette Area 0.53 0.62 0.01 0.61 0.03 between day 5 and 8 Table 53: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 54 MAB71 - Normal Growth Conditions Event No. 7331.4 7332.2 7333.5 7334.5 Control A P A P A P A P Rosette Diameter on day 5 2.24 2.52 0.02 Rosette Diameter on day 8 3.60 4.33 0.01 Rosette Area on day 5 1.54 1.88 0.07 Rosette Area on day 8 3.98 4.68 0.00 Plot Coverage on day 5 12.30 14.25 0.08 15.05 0.05 Plot Coverage on day 8 31.82 37.48 0.00 Leaf Number on day 3 5.25 5.47 0.06 5.81 0.00 Leaf Number on day 5 6.52 7.56 0.00 RGR of Leaf Number 0.16 0.17 0.01 between day 1 and 3 RGR of Leaf Number 0.12 0.12 0.10 between day 3 and 5 RGR of Leaf Number 0.12 0.12 0.01 between day 5 and 8 RGR of Rosette Area 0.53 0.61 0.04 between day 5 and 8 Table 54: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 55 MAB72 - Normal Growth Conditions Event No. 8552.4 8553.2 8553.3 Control A P A P A P Rosette Diameter on day 3 1.67 1.90 0.05 1.83 0.00 Rosette Diameter on day 5 2.24 2.67 0.00 2.55 0.01 Rosette Diameter on day 8 3.60 4.06 0.08 4.15 0.10 Rosette Area on day 3 0.89 1.20 0.00 1.02 0.00 1.08 0.02 Rosette Area on day 5 1.54 2.00 0.00 1.87 0.00 Rosette Area on day 8 3.98 5.05 0.00 4.82 0.00 Plot Coverage on day 3 7.10 9.64 0.00 8.13 0.00 8.67 0.02 Plot Coverage on day 5 12.30 16.04 0.00 14.98 0.00 Plot Coverage on day 8 31.82 40.39 0.00 38.57 0.00 Leaf Number on day 3 5.25 5.88 0.00 5.56 0.00 5.50 0.00 Leaf Number on day 8 8.92 9.38 0.02 Table 55: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 56 MAB74 - Normal Growth Conditions Event No. 7981.1 7982.4 7983.6 7983.9 Control A P A P A P A P Rosette Diameter on day 3 1.67 2.06 0.10 1.94 0.00 Rosette Diameter on day 5 2.24 2.62 0.06 Rosette Diameter on day 8 3.60 4.05 0.02 Rosette Area on day 3 0.89 1.14 0.02 Rosette Area on day 5 1.54 1.83 0.05 1.85 0.10 Rosette Area on day 8 3.98 4.46 0.03 Plot Coverage on day 3 7.10 9.13 0.02 Plot Coverage on day 5 12.30 14.62 0.03 14.79 0.08 Plot Coverage on day 8 31.82 35.66 0.01 Leaf Number on day 3 5.25 5.75 0.04 5.31 0.07 Leaf Number on day 5 6.52 6.26 0.02 7.25 0.04 Leaf Number on day 8 8.92 9.13 0.07 RGR of Leaf Number 0.12 0.13 0.08 0.12 0.03 between day 3 and 5 RGR of Rosette Area 0.46 0.33 0.00 between day 1 and 3 RGR of Rosette Area 0.36 0.42 0.07 between day 3 and 5 Biomass DW [gr] 3.07 1000 Seeds weight [gr] 0.02 0.02 0.02 0.02 0.05 Table 56: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 57 MAB76 - Normal Growth Conditions Event No. 7633.1 7635.16 Control A P A P Rosette Diameter on day 3 1.67 1.93 0.04 Leaf Number on day 3 5.25 5.44 0.02 Leaf Number on day 5 6.52 7.25 0.04 Table 57: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 58 MAB77 - Normal Growth Conditions Event No. 8212.1 8212.2 Control A P A P Leaf Number on day 3 5.25 5.63 0.00 Leaf Number on day 8 8.92 9.75 0.09 Table 58: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 59 MAB79 - Normal Growth Conditions Event No. 7324.1 7961.1 7962.2 Control A P A P A P Rosette Diameter on day 3 1.67 1.84 0.10 1.93 0.05 Rosette Diameter on day 5 2.24 2.53 0.01 Rosette Diameter on day 8 3.60 4.32 0.03 4.01 0.04 Rosette Area on day 3 0.89 1.08 0.09 Rosette Area on day 8 3.98 5.29 0.03 4.78 0.05 Plot Coverage on day 3 7.10 8.63 0.08 Plot Coverage on day 8 31.82 42.32 0.03 38.27 0.05 Leaf Number on day 3 5.25 5.75 0.04 Leaf Number on day 5 6.52 7.44 0.01 RGR of Rosette Area 0.53 0.59 0.01 between day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.00 Table 59: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

Example 7 Improved Fertilizer Use Efficiency in Arabidopsis Tissue Culture Assay

Plants transgenic to the following MAB genes were assayed for fertilizer use efficiency in a tissue culture assay: MAB115, MAB54, MAB55, MAB56, MAB57, MAB58, MAB59, MAB69, MAB70, MAB71, MAB72, MAB74, MAB76, MAB77, MAB79, MAB116, and MAB117 (the sequence identifiers of the cloned polynucleotides and their expressed polypeptides are provided in Table 7 above).

Assay 1: Plant Growth at Nitrogen Deficiency Under Tissue Culture Conditions

The present inventors have found the nitrogen use efficiency (NUE) assay to be relevant for the evaluation of the ABST candidate genes, since NUE deficiency encourages root elongation, increase of root coverage and allows detecting the potential of the plant to generate a better root system under drought conditions. In addition, there are indications in the literature that biological mechanisms of NUE and drought tolerance are linked (Wesley et al., 2002 Journal of Experiment Botany Vol 53, No. 366, pp. 13-25).

Surface sterilized seeds were sown in basal media [50% Murashige-Skoog medium (MS) supplemented with 0.8% plant agar as solidifying agent] in the presence of Kanamycin (for selecting only transgenic plants). After sowing, plates were transferred for 2-3 days for stratification at 4° C. and then grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7 to 10 days. At this time point, seedlings randomly chosen were carefully transferred to plates with nitrogen-limiting conditions: 0.5 MS media in which the combined nitrogen concentration (NH₄NO₃ and KNO₃) is 0.75 mM (nitrogen deficient conditions) or 3 mM [Norman (optimal) nitrogen concentration]. Each plate contains 5 seedlings of same event, and 3-4 different plates (replicates) for each event. For each polynucleotide of the invention at least four independent transformation events were analyzed from each construct. Plants expressing the polynucleotides of the invention were compared to the average measurement of the control plants (empty vector or GUS reporter under the same promoter) used in the same experiment.

Digital Imaging and Statistical Analysis—

Parameters were measured and analyzed as previously described in Example 5, Assay 1 above.

Tables 60-69 depict analyses of seedling parameters (as describe above) overexpressing the polynucleotides of the invention under the regulation of At6669 promoter under Nitrogene Deficiency conditions.

TABLE 60 MAB70 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 7971.3 7972.1 7974.1 7974.3 Control A P A P A P A P Dry Weight [gr] 0.01 0.01 0.00 Fresh Wight [gr] 0.10 0.18 0.04 Leaf Area on day 10 0.36 0.47 0.04 0.55 0.00 Leaf Area on day 5 0.14 0.24 0.04 Roots Coverage on day 10 5.58 7.93 0.01 7.45 0.06 Roots Coverage on day 5 1.75 2.41 0.00 2.58 0.06 Roots Length on day 10 5.19 6.16 0.00 Roots Length on day 5 2.86 3.16 0.05 RGR of Roots Coverage 0.45 0.67 0.05 between day 5 and 10 RGR of Roots Coverage 0.81 2.35 0.02 2.01 0.06 2.10 0.05 2.23 0.02 between day 1 and 5 RGR of Roots Length 0.16 0.24 0.04 between day 1 and 5 RGR of Roots Length 0.20 0.50 0.00 0.48 0.00 0.56 0.00 0.58 0.00 between day 5 and 10 Table 60: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 61 MAB71 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 7331.5 7332.2 7333.5 7334.4 Control A P A P A P A P Dry Weight [gr] 0.01 0.01 0.00 0.01 0.01 0.01 0.01 0.01 0.01 Fresh Wight [gr] 0.10 0.22 0.00 0.18 0.02 0.13 0.09 Leaf Area on day 10 0.36 0.55 0.00 0.52 0.00 Leaf Area on day 5 0.14 0.28 0.00 0.21 0.00 Roots Coverage on day 10 5.58 8.19 0.05 9.47 0.03 Roots Coverage on day 5 1.75 2.33 0.10 2.99 0.02 Roots Length on day 10 5.19 6.69 0.03 Roots Length on day 5 2.86 3.84 0.01 RGR of Roots Length 0.16 0.20 0.07 between day 1 and 5 RGR of Roots Length 0.20 0.66 0.05 0.26 0.08 between day 5 and 10 Table 61: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 62 MAB74 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 7982.4 7983.9 Control A P A P Roots Coverage on day 10 5.58 9.76 0.06 Roots Length on day 10 5.19 6.70 0.00 RGR Leaf Area between day 5 0.30 0.44 0.09 and 10 RGR of Roots Coverage 0.45 0.63 0.08 between day 5 and 10 Table 62: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 63 MAB76 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 7635.4 Control A P RGR of Roots Coverage between day 5 and 10 0.45 0.70 0.07 RGR of Roots Length between day 1 and 5 0.16 0.26 0.07 Table 63: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 64 MAB77 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 7931.11 8211.8 8212.2 Control A P A P A P Dry Weight [gr] 0.01 0.01 0.00 Fresh Wight [gr] 0.10 0.13 0.03 0.14 0.01 Roots Coverage on day 5 1.75 2.26 0.03 RGR of Roots Coverage 0.45 0.71 0.01 0.75 0.05 between day 5 and 10 RGR of Roots Length 0.16 0.24 0.03 between day 1 and 5 RGR of Roots Length 0.20 0.31 0.05 0.99 0.04 0.86 0.08 between day 5 and 10 Table 64: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 65 MAB79 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 7323.3 7324.1 7961.1 Control A P A P A P Dry Weight [gr] 0.01 0.01 0.00 0.01 0.03 0.01 0.01 Fresh Wight [gr] 0.10 0.16 0.00 0.11 0.10 RGR of Roots Coverage 0.45 0.71 0.09 between day 5 and 10 RGR of Roots Coverage 0.81 3.11 0.00 between day 1 and 5 RGR of Roots Length 0.20 0.67 0.00 0.49 0.09 between day 5 and 10 Table 65: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 66 MAB115 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 8561.2 8564.2 8565.1 Control A P A P A P Dry Weight [gr] 0.005 0.006 0.00 0.010 0.00 0.009 0.00 Leaf Area on day 10 0.24 0.27 0.00 Leaf Area on day 5 0.35 0.38 0.01 Roots Coverage on day 10 1.67 2.13 0.02 Roots Coverage on day 5 3.38 4.80 0.03 Roots Length on day 10 2.39 2.74 0.00 Roots Length on day 5 3.46 4.22 0.02 RGR Leaf Area between 0.37 0.43 0.00 0.48 0.00 day 5 and 10 RGR Leaf Area between 0.16 0.19 0.00 day 1 and 5 RGR of Roots Coverage 1.89 3.21 0.00 2.23 0.02 3.47 0.01 between day 5 and 10 RGR of Roots Coverage 0.33 0.35 0.00 0.43 0.00 0.44 0.00 between day 1 and 5 RGR of Roots Length 0.37 0.56 0.02 0.53 0.06 0.68 0.00 between day 1 and 5 RGR of Roots Length 0.15 0.17 0.00 0.18 0.00 between day 5 and 10 Table 66: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 67 MAB54 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 8181.2 8182.2 8185.3 Control A P A P A P Dry Weight [gr] 0.005 0.009 0.00 0.008 0.00 0.007 0.00 Roots Coverage on day 10 1.67 1.80 0.04 Roots Coverage on day 5 3.38 4.27 0.02 3.40 0.00 Roots Length on day 10 2.39 2.52 0.01 Roots Length on day 5 3.46 4.11 0.02 3.50 0.00 RGR Leaf Area between 0.37 0.45 0.00 day 5 and 10 RGR Leaf Area between 0.16 0.17 0.04 0.19 0.00 day 1 and 5 RGR of Roots Coverage 1.89 3.59 0.00 3.60 0.01 2.20 0.01 between day 5 and 10 RGR of Roots Coverage 0.33 0.52 0.00 0.55 0.00 0.36 0.00 between day 1 and 5 RGR of Roots Length 0.37 0.70 0.00 0.73 0.00 0.51 0.02 between day 1 and 5 RGR of Roots Length 0.15 0.21 0.01 0.22 0.01 0.17 0.00 between day 5 and 10 Table 67: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 68 MAB57 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 6912.14 6912.20 6912.60 Control A P A P A P Roots Coverage on day 7 5.55 6.71 0.10 Roots Coverage on day 14 15.02 17.33 0.05 Roots Length on day 7 4.93 5.54 0.10 6.12 0.02 6.34 0.02 Roots Length on day 14 7.83 8.76 0.02 Fresh Weight 0.19 0.24 0.09 Table 68: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

TABLE 69 MAB72 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 8552.1 8552.4 8553.2 Control A P A P A P Dry Weight [gr] 0.005 0.01 0.00 0.01 0.00 0.01 0.00 Leaf Area on day 10 0.24 0.24 0.01 Roots Coverage on day 10 1.67 1.84 0.01 1.93 0.02 1.89 0.03 Roots Coverage on day 5 3.38 3.60 0.04 3.90 0.06 4.50 0.00 Roots Length on day 10 2.39 2.48 0.01 Roots Length on day 5 3.46 3.70 0.04 3.65 0.04 3.84 0.00 RGR Leaf Area between 0.37 0.47 0.00 0.39 0.00 day 5 and 10 RGR Leaf Area between 0.16 0.20 0.09 day 1 and 5 RGR of Roots Coverage 1.89 1.93 0.03 2.29 0.06 3.04 0.01 between day 5 and 10 RGR of Roots Coverage 0.33 0.35 0.00 0.52 0.00 between day 1 and 5 RGR of Roots Length 0.37 0.55 0.01 between day 1 and 5 RGR of Roots Length 0.15 0.16 0.00 0.18 0.00 0.22 0.01 between day 5 and 10 Table 69: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

Example 8 Transgenic Tomato and Arabidopsis Plants Show Improved Tolerance to Salt and Water-Deficiency Stresses Under Field Conditions

To test the impact of AQP TIP2 genes on plant's stress tolerance, the present inventors have previously cloned and overexpressed a polynucleotide which comprises the nucleic acid sequence set forth by SEQ ID NO:2827 (also known as ABST36 set forth by SEQ ID NO:13 in WO2004/104162; or S1TIP2; 2) and which encodes the TIP2 polypeptide set forth by SEQ ID NO:2828 (which comprises the consensus sequence TLXFXFAGVGS; SEQ ID NO:2826). The nucleic acid constructs which comprises the ABST36 polynucleotide under the regulation of the constitutive Arabidopsis At6669 promoter (SEQ ID NO: 2823) (further referred to as the At6669::ABST36 construct) was further transformed into tomato (Solanum lycopersicum) as a model crop plant (Tom-ABST36), as well as into Arabidopsis thaliana. Four independent, T₂ transgenic tomato genotypes, overexpressing ABST36 in heterozygous form, were evaluated for their tolerance to salt and water deficiency in two different salt-stress field trials and one water-deficiency-stress field trial consisting of two water-deficiency regimes. Transgenic genotypes in each field trial were compared to their null-segregant counterparts as controls.

Materials and Experimental Methods

Tomato Salt-Stress Field Trial—

All field trials were performed in a light soil, in an open field (net-house) near Rehovot, Israel. The F1 hybrids of four independent events of the cross between ABST36-transgenic MicroTom plants and M82 tomato plants were grown for the first 3 weeks in a nursery under normal irrigation conditions. The seedlings were then transplanted into rows and grown in a commercial greenhouse. The salt-stress trial was divided into four blocks. In each block, two different irrigation systems were established: a normal water regime for tomato cultivation and a continuous irrigation with saline water (addition of 180 to 200 mM NaCl). Each block consisted of a total of 60 plants divided as follows: six plants per event and six seedling null segregants were planted in the control row and a similar number of plants were planted in the salt-stressed row. At the stage of about 80% red fruits in planta, fruit yield, plant fresh weight, and harvest index were calculated. Harvest index was calculated as yield/plant biomass.

Tomato Water-Deficiency-Stress Field Trial—

All field trials were performed in a light soil, in an open field (net-house) near Rehovot, Israel. The F1 hybrids of the four independent events were initially grown as described above. Three-week-old seedlings were transplanted to a net-greenhouse. The experiment was structured in four blocks containing three rows irrigated with different water levels and intervals (WLI-0, WLI-1, WLI-2). In each block, six transgenic plants per event analyzed and six non transgenic plants were transplanted in each row. Seedlings were transplanted after 4 weeks into wet soil. The amount of water used to uniformly irrigate before transplanting reached maximum water capacity [20% weight per weight (w/w)] at 60 cm depth, but without the creation of water overload. Each plant was transplanted near a dripper, with a 30-cm distance between plants, giving a total density of 2,600 plants per 1,000 m², according to a commercial growth protocol. Soil water capacity was measured using the standard procedures by sampling soil from the following three depths: 0 to 20 cm, 20 to 40 cm, and 40 to 60 cm. The water content in these soil layers was measured routinely every week. The soil contained 5% hygroscopic water while the maximum water capacity of the soil was 20%. All fertilizers were applied in the soil prior to plant transplantation. The amount of both phosphorus and potassium was calculated to be sufficient for all seasons. Nitrogen was applied as recommended, equally to all treatments, through the irrigation system. Each row contained three dripping irrigation lines creating a coverage of nine drippers per 1 m². The water control was performed separately for each treatment. The soil was dried completely before the beginning of the experiment. The different water regimes were begun only 4 weeks after transplanting when plants initiated the flowering stage. The amount of water supplied every week during the assay was calculated at the beginning of every week following the recommendations of standard growth protocols. WLI-0 treatment (control) received the recommended total weekly irrigation volume divided into three irrigations. WLI-1 was irrigated three times a week, but the amount of water supplied was half that supplied to WLI-0. At the end of every week, WLI-1 plants received the amount of water required to reach maximum soil water capacity. WLI-2 plants were irrigated only once a week, at the beginning of the week. The water-stress experiment lasted throughout the flowering period (23 days), corresponding to four cycles of the above-described stresses. Afterwards, all treatments received the recommended amount of water. The calculated water amount was equal to the difference between the water contents in dry soil and in soil with maximum water capacity. At the end of each stress cycle, the water amounts were compared between treatments according to actual water content in the soil (S3). During the stress period, treatments WLI-1 and WLI-2 received a total of 75% less water than the controls (WLI-0).

Experimental Results

Transgenic Plants Exhibit Increased Tolerance to Salt Stress—

To induce salt-stress, transgenic and control tomato plants were continuously irrigated in field trials with 180 to 200 mM NaCl. As shown in FIGS. 3 a-c, 3 g-j and Table 70 below, Tom-ABST36 plants appeared to be more vigorous in all of the experiments than the control plants, which were smaller and showed severe symptoms of leaf and shoot necrosis (see for example, FIG. 3 j). This was also associated with higher fruit yield in Tom-ABST36 plants relative to controls (FIG. 3 a).

TABLE 70 Salt-stress field trial Control 180 mM NaCl Plant FW Fruit yield Harvest Plant FW Fruit yield Harvest (tn/acre) (tn/acre) index (tn/acre) (tn/acre) %* index SlTIP2; 2 ND 24.0 ND 2.8 ^(a) 8.0 ^(a) 110% 2.8 WT ND 24.0 ND 1.4 ^(b) 3.8 ^(b)  0% 2.7 Table 70: Total yield (ton fruit/acre), plant fresh weight (FW), and harvest index were calculated for TOM ABST36 vs. control plants growing in the field under salt-stress conditions (180 mM NaCl). Results are the average of four independent events. ^(a, b) Values in a column followed by different superscript letters are significantly different.

Transgenic Plants Exhibit Increased Tolerance to Water-Deficiency Stress—

Transgenic plants subjected to water-deficiency stress exhibited a significantly higher (26%, p≦0.05) plant biomass compared to control plants (FIG. 3 e). Moreover the Tom-ABST36 plants showed a significant (up to 21%, p≦0.05) increment of fruit yield under water-deficient regimes (water level intervals WLI-1), while under normal irrigation, the yield improvement was even higher (27%, p≦0.05; FIG. 3 d). The harvest index of the Tom-ABST36 plants was also higher when plants grew under regular and WLI-1 conditions while it remained similar to control when the water-deficient regime consisted of once-a-week irrigation (WLI-2) (FIG. 3 f).

The results from the three field trials provided strong evidence that the tomato Tom-ABST36 plants show improved tolerance to salt and water-deficiency stress relative to the control plants, which is translated into significant increments in plant biomass and more importantly, fruit yield.

Arabidopsis Salt-Stress Green House Trial—

A complementary experiment with transgenic Arabidopsis plants expressing the ABST36 construct showed increased tolerance to a salt stress of 150 mM NaCl compared to control plants, as reflected in 42% higher fresh biomass and 60% higher dry biomass (Table 71 below).

In-Vitro Salt-Stress Assay—

Seeds of transgenic Arabidopsis plants harboring the At6669::ABST36 construct or 35S::GUS construct (which was used as control) were sown in ½ MS media containing 40 mg/l kanamycin for selection. Selected seedlings were sub-cultured to ½ MS media with 0 or 150 mM NaCl. Plants were grown for a period of 3 weeks. Results are the average of four independent events that were analyzed in four repeats. For the determination of shoot dry weight, shoot plants were collected and dried for 24 hours at 60° C. and then weighed.

TABLE 71 Arabidopsis salt-stress assay 0 mM NaCl 150 mM NaCl Plant FW Plant DW Plant FW Plant DW Lines (mg) (mg) (mg) (mg) SlTIP2; 2 408.28^(a) 23.52^(a) 68.55^(a) 4.4^(a) WT 394.36^(a) 22.63^(a) 48.12^(b) 2.7^(b) Table 71. Arabidopsis seedlings were grown in 0 and 150 mM NaCl under tissue-culture conditions. Shown are the fresh weight (FW) and total dry weight (DW) (both measured in milligrams) of ABST36 (SEQ ID NO: 2827) transgenic or wild type controls under normal conditions (0 mM NaCl) or salinity stress (150 mM NaCl). ^(a,b)Values in a column followed by different superscript letters are significantly different at P < 0.05

Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.

All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting. 

What is claimed is:
 1. A method of increasing abiotic stress tolerance, fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant as compared to a non-transformed plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 32, 2766, 31 and 2767, thereby increasing the abiotic stress tolerance, fertilizer use efficiency (FUE), biomass, vigor and/or yield of the plant as compared to the non-transformed plant, wherein said abiotic stress comprises salinity and fertilizer deficiency.
 2. The method of claim 1, wherein said exogenous polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2755, 6, 5 and
 2756. 3. An isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide which comprises an amino acid sequence selected from the group consisting of SEQ ID NO:32, 2766, 31, and
 2767. 4. The isolated polynucleotide of claim 3, wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NO:2755, 6, 5 and
 2756. 5. A nucleic acid construct, comprising the isolated polynucleotide of claim 3 and a promoter for directing transcription of said nucleic acid sequence.
 6. A nucleic acid construct, comprising the isolated polynucleotide of claim 4 and a promoter for directing transcription of said nucleic acid sequence.
 7. A plant cell comprising the nucleic acid construct of claim
 5. 8. A plant cell comprising the nucleic acid construct of claim
 6. 9. An isolated polypeptide, comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 32, 2766, 31, and
 2767. 10. A plant cell exogenously expressing the isolated polypeptide of claim
 9. 11. The method of claim 1, further comprising growing the plant expressing said exogenous polynucleotide under the abiotic stress.
 12. The plant cell of claim 7, wherein said promoter is heterologous to the plant.
 13. The nucleic acid construct of claim 5, wherein said promoter is a constitutive promoter.
 14. The plant cell of claim 7, wherein said plant cell forms a part of a plant. 